Provided herein are systems and methods for a point of care apparatus. The point of care apparatus includes a fluid container for receiving a biosample, an intermediate cap, and a cartridge having at least one microfluidic channel.

A receptacle is designed for a chamber for an active substance. The receptacle includes two main elements. A first element is a transverse wall with a first main side facing an interior of the chamber, and a peripheral wall formed with the first main side of the transverse wall. A second element includes a bottom wall and a sheath extending from the bottom wall. The peripheral wall and the sheath surround one another and are in contact with each other so that the chamber is formed between the bottom wall, the transverse wall, the peripheral wall and/or the sheath. At least one ventilation groove is provided between the peripheral wall and the sheath form a ventilation channel connecting the chamber with the outside atmosphere.

Provided is a reagent cooler that prevents occurrence of condensation and further uniforms a temperature in the cooler with low power consumption and a simple structure. The reagent cooler includes a refrigerant pipe that is disposed inside an outer wall of the reagent cooler 103 and circulates a refrigerant inside the outer wall; a blowing pipe 109 that is disposed inside the outer wall and guides outside air existing outside the reagent cooler to inside of the reagent cooler; and a blowing unit 114 that is disposed at the blowing pipe and diffuses the outside air into the inside of the reagent cooler through the blowing pipe. With the outside air cooled by the outer wall and taken into the inside of the reagent cooler, the reagent cooler is positively pressurized and the internal temperature is made uniform.

According to an example aspect of the present invention, there is provided a box-shaped package, comprising: a base, and a cover configured to be placed on the base to close the package, wherein in a closed configuration of the package, the interface between the base and the cover is gas-tight; the base and/or the cover comprise one or more gas-permeable zones which are capable of passing gases, such as air, into and out from the package, particularly in the closed configuration.

A sample testing apparatus includes a sample tray defining a planar surface and a plurality of wells recessed relative to the planar surface, and a lid member configured to be sealed about the planar surface of the sample tray. The lid member includes an adhesive layer configured to be sealed to the planar surface of the sample tray, a breathable film layer disposed about the adhesive layer, and a backing layer disposed about the breathable film layer. Methods of using the sample testing apparatus for testing a sample and kits to facilitate such testing are also provided.

The present invention may include an upper cap having an opening/closing member for opening and closing an inlet through which a blood is injected; a main body housing having an open upper end outer surface thereof assembled with the upper cap, and having an inner space in which the blood is filled with; an adjustment housing that is coupled to the main body housing to seal the open lower end of the main body housing, and is movably assembled with respect to the main body housing so that a buffy coat layer is positioned on a neck portion; and a side cap fixed to the neck portion by forming a needle hole through which an end of an injection needle for extraction enters and exits in area corresponding to a sealing member to seal an extraction hole formed through the neck portion.

A method for exosome separation and extraction by stacked centrifugal filtration. It is used in molecular biology and clinical examination and comprises an exosome separation and extraction kit consisting of the stacked centrifugal filtration device, an incubation buffer and a protease K. The sample to be tested is incubated at room temperature using the incubation buffer and an appropriate amount of protease K, followed by centrifugation in a centrifuge matching the stacked centrifugal filtration device. After mixing thoroughly, the retained liquid in the ultrafiltration tube is collected to obtain the exosomes. The method needs no large experimental equipments except for a centrifuge, which has a low cost and which is convenient and fast, with short operation time and the possibility of carrying out parallel operations with a large number of samples. The high purity exosomes obtained by the method can meet the demand of large-scale clinical applications.

A container for storing a biological sample is disclosed. The container includes a housing having a closed end, an open end, and a sidewall extending therebetween defining a container interior. The container has a removable closure for enclosing the open end of the housing and a sample holder for housing a biological sample detachably connected to the closure and insertable within the container interior. A port is disposed within the closure adjacent the sample holder to allow fluid to pass therethrough into the container interior. An injection device for engaging the port may also be provided. A first fluid may be initially provided within the container interior and a second fluid may be subsequently injected by the injection device through the port into the container interior.

The present invention relates to systems that utilize a combination of immunoassay and magnetic immunoassay techniques to detect an analyte within an extended range of specified concentrations. In particular, a device is provided for detecting an analyte in a biological sample. The device includes a first electrochemical sensor positioned on a substrate. The first electrochemical sensor includes an immobilized layer of antibody configured to bind to the analyte. The device further includes a second electrochemical sensor positioned adjacent to the first electrochemical sensor on the substrate, and a magnetic material that generates a magnetic field aligned with respect to the second electrochemical sensor. The magnetic field captures magnetic beads that have an immobilized layer of antibody configured to bind to the analyte, and concentrates the magnetic beads on or near a surface of the second electrochemical sensor.

A sample analysis cup assembly, system and method for purging including a cell body, including a top end; a bottom end; a cell body wall extending axially from the top end to the bottom end; a transverse wall adjacent the top end, including a plurality of apertures extending therethrough; and a raised portion on the transverse wall including a central aperture extending therethrough; a rotatable cap, including a top surface; a bottom surface; and a series of apertures extending from the top surface through the bottom surface, the rotatable cap being structured to engage with the top end of the cell body; and a ring member structured to couple with the bottom end of the cell body are provided.