A digital microfluidics (DMF) device including an FET-biosensor (FETB) and method of field-effect sensing is closed. In some embodiments, the DMF device may include one or more FETBs integrated into the top substrate, the bottom substrate, or both the top and bottom substrates of the DMF device. In some embodiments, the DMF device may include one or more “drop-in” style FETBs in the top substrate, the bottom substrate, or both the top and bottom substrates of the DMF device. In some embodiments, the DMF device, FETB, and method of field-effect sensing provide active-matrix control integrated into an active-matrix DMF device. Further, a microfluidics system for and method of using the DMF device including at least one FETB is provided.

The invention is directed to apparatus and methods for delivering multiple reagents to, and monitoring, a plurality of analytical reactions carried out on a large-scale array of electronic sensors under minimal noise conditions. In one aspect, the invention provides method of improving signal-to-noise ratios of output signals from the electronic sensors sensing analytes or reaction byproducts by subtracting an average of output signals measured from neighboring sensors where analyte or reaction byproducts are absent. In other aspects, the invention provides an array of electronic sensors integrated with a microwell array for confining analytes and/or particles for analytical reactions and a method for identifying microwells containing analytes and/or particles by passing a sensor-active reagent over the array and correlating sensor response times to the presence or absence of analytes or particles. Such detection of analyte- or particle-containing microwells may be used as a step in additional noise reduction methods.

An expandable array and methods of maintaining a biological sample within an expandable array are provided. The expandable array includes a plurality of receptacles configured to receive a biological sample and a plurality of beams comprising a programmable material. Each beam of the plurality of beams is located between and connects at least two receptacles. The programmable material can be a shape-memory polymer or a magnetoactive material that transitions the plurality of beams from an extended state to a contracted state upon application of a stimulus.

Examples of the disclosure relate to an apparatus for analysing fluid samples. The apparatus is sized and shaped so that it can fit into an input port of an electronic device. The input port could be an existing port of the electronic device such as an input port for a memory card or a charger. The electronic device can be configured with a heat transfer means so that, when the apparatus is inserted into the electronic device, heat from the electronic device can be used to control the temperature of a fluid sample within the apparatus. This can enable the reaction conditions within the apparatus to be controlled.

Provided herein are methods for inkjet printing objects, including objects which may be used as elements of microfluidic devices. The microfluidic devices incorporating the elements are also provided. Such microfluidic devices include those configured to quantify the expression and activity of exosomal matrix metalloprotease, MMP14. These microfluidic devices may be used in methods of monitoring breast cancer in patients having breast cancer.

The present invention relates to a cartridge for sandwich ELISA pre-loaded with antigen customized detection reagent to more efficiently perform the sandwich ELISA and a sandwich ELISA device using the cartridge. A cartridge for sandwich ELISA according to the present invention is arranged in rows and columns, and includes a body in which wells accommodating reagents are formed, a pin-assy including pins that have a capture antibody coated on one ends thereof, and a provider that moves the pin-assy to provide the well with the pin to allow the reagents and the capture antibody to be into contact and react with each other.

A system configured to determine the load in a liquid sample of predetermined antigens is provided. The system comprises a measurement chamber configured for receipt therein of the liquid sample, a sensor circuit, and an analysis unit. The sensor circuit comprises a plurality of working electrodes, each comprising antibodies on its surface associated with one of the predetermined antigens, at least one reference electrode, and at least one counter electrode. Proximal ends of the electrodes are disposed on a reading zone of the sensor circuit, the reading zone being disposed within the measurement chamber. The analysis unit is configured to facilitate the determination of the load of each of the antigens by measuring electrical properties of the electrodes.

An analysis apparatus including a stage, an analysis device placed on the stage and including receiving sections which accommodate a sample and a reagent for biochemical reaction, and are communicated with one another through a flow path having an inlet and an outlet, a liquid introduction section which is connected to the inlet and supplies into the flow path the sample, the reagent, and an sealing liquid for sealing each of the receiving sections, and a waste liquid storage section which is connected to the outlet and stores as waste liquid an excess of the sample and the reagent and a part of the sealing liquid supplied to the flow path, an optical system which includes an objective lens, emits excitation light to the receiving sections and allows observation of fluorescence generated in the receiving sections by the excitation light, and a control unit that controls such that the sealing liquid and the excess of the sample and the reagent form an interface in the waste liquid storage section, and that the interface is formed at a distance not less than a fluorescence-obtainable distance from a bottom of the receiving sections.

The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

A portable detector is disclosed for detecting certain analytes of interest, such as genetic material (e.g., nucleic acids). The detector includes a reading component for the detection of the analytes, and control circuitry for controlling operation of the reading component. Processing circuitry may be included to perform both primary analysis of acquired data, and where desired, secondary analysis. Where desired, some or all of the computationally intensive tasks may be off-loaded to enhance the portability and speed of the device. The device may incorporate various types of interface, technologies for reading and analysis, positioning system interfaces, and so forth. A number of exemplary use cases and methods are also disclosed.