A microfluidic device includes a lower casing and an upper casing covering the lower casing. The lower casing includes a lower base wall having a top surface and a plurality of spaced-apart columns that protrude upwards from the top surface. The upper casing includes an upper base wall. A first gap between the upper base wall and a column top surface of each of the columns is large enough to permit passage of large biological particles of a liquid sample, and a second gap between any two adjacent ones of the columns is not large enough to permit passage of the large biological particles and is large enough to permit passage of small biological particles of the liquid sample.

A method includes mounting an integrated electro-microfluidic probe card to a device area on a bio-sensor device wafer, wherein the electro-microfluidic probe card has a first major surface and a second major surface opposite the first major surface. The method further includes electrically connecting at least one electronic probe tip extending from the first major surface to a corresponding conductive area of the device area. The method further includes stamping a test fluid onto the device area. The method further includes measuring via the at least one electronic probe tip a first electrical property of one or more bio-FETs of the device area based on the test fluid.

A point-of-care diagnostic system that includes a cartridge and a reader. The cartridge can contain a patient sample, such as a blood sample. The cartridge is inserted into the reader and the patient sample is analyzed. The reader contains various analysis systems, such as an electrophoresis detection system that uses electrophoresis testing to identify and quantify various components of the blood sample. The reader can process data from the various patient sample analysis to provide interpretative results indicative of a disorder, condition, disease and/or infection of the patient.

Provided herein are systems, methods, and articles of manufacture for collecting and merging two different size droplets using a substrate comprising a plurality of trapping sites. In certain embodiments, provided herein are systems composed of a plurality of larger droplets and smaller droplets and a substrate comprising a plurality of trapping sites where each trapping site is configured to trap only one of the larger droplets and only one of the smaller droplets when the larger droplet is already present at the trapping site. In particular embodiments, the larger and/or smaller droplets are sorted prior to being contacted with the substrate to ensure they contain the desired component (e.g., cell or barcoded bead). In other embodiments, each trapping site is composed of one or multiple fluidically linked capture wells. In some embodiments, collected larger and smaller droplets are merged (e.g., via a demulsifier or electricity).

A test strip for sampling a bodily fluid may include multiple layers of a substrate material, an adhesive between at least some of the multiple layers, and a microfluidic channel formed between at least some of the multiple layers. The test strip may further include multiple electrodes on one of the multiple layers, positioned and partially exposed within the microfluidic channel, an additional material positioned at or near an entrance to the microfluidic channel, to selectively limit the flow of at least one of bubbles or debris into the microfluidic channel, and at least one exit port in at least one of the multiple layers to allow for release of pressure from the test strip. In some embodiments, the test strip is a saliva analysis test strip. In some embodiments, the test strip includes multiple exit ports to prevent blockage of sample flow.

An apparatus is provided for sensing a molecule in a sample. The apparatus utilizes an electric field to draw molecules from a first chamber through an aperture, defined by a chemical layer, into a second chamber. The apparatus can detect a DNA molecule with, for example, 4, 5, or 6 unique base pairs. As molecules pass through the aperture, a sensor detects or measures a change in an electric parameter used to generate the electric field, thereafter translating the change in the electric parameter into information about the molecule. A divider element separates the first and second chambers and supports a chemical layer defining the aperture. The apparatus enables detection or measurement of molecules over prolonged time at a higher electric field strength than other nanopores, due to a combination of the shape of the divider, structural elements thereon, and thickness of the chemical layer at the aperture.

A device, liquid handling cartridge and related method for detecting the presence or absence of a chemical or biological target within a sample. The method includes the steps of: providing an electrochemical cell with a first electrode module and a second electrode; providing an electronic component between the first electrode module and the second electrode; introducing the sample into the electrochemical cell; measuring the potential difference between the first electrode module and second electrode; and confirming the presence of the chemical or biological target if the measured potential difference exceeds a predetermined threshold value.

A cartridge for use with an instrument to perform measurement of a fluid, including a digital microfluidics substrate comprising a plurality of electrowetting electrodes operative to perform droplet operations on a liquid droplet in a droplet operations gap; a top plate separated from the digital microfluidics substrate to form a droplet operations gap and comprising openings for injecting liquids into the droplet operations gap; a fiber assembly comprising a fiber optic probe projecting into the droplet operations gap and having a sensing end situated in proximity with one or more of the electrowetting electrodes.

In one example in accordance with the present disclosure, a fluid ejection die is described. The fluid ejection die includes a fluid feed slot to deliver fluid from a reservoir to an array of ejection chambers fluid connected to the fluid feed slot. Each ejection chamber includes at least one fluid actuator and an opening through which fluid is to be ejected. The fluid ejection die also includes a number of antechambers. An antechamber includes sidewalls that curve inward.

A method for detecting at least one analyte by electrochemical detection, a working electrode of an analyte sensor and an analyte sensor for detecting at least one analyte in a sample by electrochemical detection. The method comprises contacting a fluid sample suspected to comprise the at least one analyte with the surface of an electrode comprising a binding agent capable of binding to the analyte; contacting the fluid sample with a detection agent comprising a further binding agent capable of binding to the analyte and a label, the label comprising a metal nanoparticle with a standard redox potential E° between 0 V and 1.2 V forming a detection complex on the surface of the electrode comprising the binding agent, the detection agent and the analyte precipitating at least a part of the label onto the electrode surface; and detecting the analyte by electrochemical detection.