A substrate for use in manufacture of a production master plate for production of a detection disc for carrying samples in an apparatus for detection of microscopic objects in a fluid, the substrate having a channel and separate focus structure, wherein the focus structure is a groove.

A microfluidic device and method comprises a rotatable substrate configured to be rotated at various angular velocities in order to accomplish a series of media exchanges with a sample chamber for containing a sample. A plurality of media reservoirs and waste reservoirs are connected to the sample chamber via channels forming capillary valves in which the capillary valves have varying burst frequencies of rotation of the substrate. The substrate can then be rotated through a series of angular velocities to automate media exchanges between the sample chamber and the respective media reservoirs and waste reservoirs. A pair of media reservoirs are located radially inward of the sample chamber and a pair of waste reservoirs are located radially outward of the sample chamber in an X configuration such that centrifugal forces cause fluid media to open the capillary valves and flow among the sample chamber, media reservoirs and waste reservoirs.

An example assembly includes a target holder that retains a target in a detection region. Areflective surface reflects at least part of a focused spot of light to provide resultant light. An irradiation system irradiates at least part of the detection region with the focused spot of light. A motion system causes motion of the focused spot of light relative to the reflective surface. A detection system detects the resultant light. An example device, e.g., a lab-on-chip, includes a substrate, a sample inlet, and a reflective grating. The grating is retains a fluidic sample in a detection region fluidically connected to the sample inlet. The detection region is operatively arranged with respect to the reflective grating so that at least a portion of light passing through the detection region towards the reflective grating also passes through the detection region after reflecting off the reflective grating.

The invention relates to a microfluidic system for processing biological samples comprising a holding chamber adapted for holding a fluid and to be rotated on a platform, said holding chamber comprising an outlet through which fluid flow is controlled by an acceleration-primed valve system, wherein the acceleration-primed valve system comprises a capillary valve and an outlet channel. The invention provides a novel valving system, which retains fluids at low angular velocities, removes the need for hydrophilic surfaces, minimizes disc real-estate and optimizes certain microfluidic processes done in the holding chamber.

The present invention discloses an apparatus for conducting an assay, wherein the apparatus comprises a turntable (1) for receiving an assay disc (100) and to control an assay on said assay disc by rotational movement of said turntable, wherein said turntable comprises: one or more heater modules (4) to apply heat to one or more specific parts of said assay disc during rotation; a heater controller (54) to select a required heater and to control the temperature thereof, and an IR transceiver (30, 55) to allow instructions and/or heating parameters to be transferred to the heater controller wirelessly. More specifically, the present invention discloses, an apparatus for conducting an assay, wherein the heating location and rate of heating/cooling at a particular location on the assay disc may be controlled.

A sample test method, microfluidic device, and test device efficiently and accurately compensates for interference of an interfering substance present in a sample using optical measurement without addition of a separate reagent for detecting the interfering substance. The sample test method includes: measuring an optical characteristic value of a target substance present in a sample; measuring an optical characteristic value of an interfering substance present in the sample; and determining a concentration of the target substance for which interference of the interfering substance is compensated for based on the optical characteristic value of the interfering substance.

The present disclosure relates to a microfluidic-based analyzer, including a drive module and a microfluidic disc. On the microfluidic disk, a capillary is connected between a mixing chamber and a waste chamber. More particularly, the capillary is connected to the mixing chamber through a first access on the first radius of the microfluidic disc, and the capillary is connected to the waste chamber through a second access on the second radius of the microfluidic disk. Specifically, a turn of the capillary is disposed between the first access and the second access, in which a folding is configured on a third radius of the microfluidic disc. Overall, the aforementioned microfluidic-based analyzer is able to be operated in different rotational speeds and is capable of evacuating the mixing chamber and enhancing the washing efficiency.

A substrate for use in manufacture of a production master plate for production of a detection disc for carrying samples in an apparatus for detection of microscopic objects in a fluid, the substrate having a channel and separate focus structure, wherein the focus structure is a groove.

An analyzing device includes a rotation axis, and a separating cavity having first, second and third sidewalls. The first sidewall connects the second and the third sidewalls, and the second sidewall extends from a position connecting the first sidewall and the second sidewall toward the rotation axis. The third sidewall has a first end and a second end, with the first end located closer to the rotation axis than the second end. The analyzing device further includes a measurement channel configured to allow a sample liquid to be filled therein by a capillary force and having a capacity of a first predetermined amount.

Devices and methods for handling liquids are provided. The devices and methods make use of centrifugal forces to drive liquid flow and facilitate one or more of the mixing, metering and sequencing of liquids, for example on a microfluidic device.