A sample test method, microfluidic device, and test device efficiently and accurately compensates for interference of an interfering substance present in a sample using optical measurement without addition of a separate reagent for detecting the interfering substance. The sample test method includes: measuring an optical characteristic value of a target substance present in a sample; measuring an optical characteristic value of an interfering substance present in the sample; and determining a concentration of the target substance for which interference of the interfering substance is compensated for based on the optical characteristic value of the interfering substance.

An example assembly includes a target holder that retains a target in a detection region. A reflective surface reflects at least part of a focused spot of light to provide resultant light. An irradiation system irradiates at least part of the detection region with the focused spot of light. A motion system causes motion of the focused spot of light relative to the reflective surface. A detection system detects the resultant light. An example device, e.g., a lab-on-chip, includes a substrate, a sample inlet, and a reflective grating. The grating is retains a fluidic sample in a detection region fluidically connected to the sample inlet. The detection region is operatively arranged with respect to the reflective grating so that at least a portion of light passing through the detection region towards the reflective grating also passes through the detection region after reflecting off the reflective grating.

Provided herein are systems and methods for separation of heterogeneous analyte. In some embodiments, the systems and methods herein utilize an analyte holding portion that receives a volume of analyte. The analyte holding portion may include an analyte receiving portion and a microfluidic separation channel, A rotatable carrier holding the analyte holding portion may be rotated by a rotational actuator so as to apply a centrifugal force to the volume of analyte such that the analyte is separated into at least two components.

According to the invention, generally, a method for making a microfluidic aliquoting (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. Design improvements including an injection mold design for an insert and a base and a multiplex hole punch allow for rapid fabrication of the MA chip.

According to the invention, generally, a microfluidic aliquot (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. An insert and a base are configured to create an MA chip.

Described embodiments provide devices, systems and methods for sequencing liquid flow in response to a driving force by entrapping and releasing gas between volumes of liquid in a controlled manner. In one particular form, a centrifugal lab on a disk device is provided to drive liquid flow and sequencing by virtue of the centrifugal force and in one particular form a radially inward bend conduit is used in connection with controllably trapping and releasing gas between liquid volumes.

The present invention provides an analyte detection system for detecting target analytes in a sample. In particular, the invention provides a detection system in a rotor or disc format that utilizes a centrifugal force to move the sample through the detection system. Methods of using the rotor detection system to detect analytes in samples, particularly biological samples, and kits comprising the rotor detection system are also disclosed.

A detection plate includes a track region having a bottom surface having a groove provided therein, and a well apart from the track region. The well has a bottom surface. The bottom surfaces of the track region and the well are disposed substantially on a plane. This detection plate detects a specimen rapidly and highly accurately with a simple structure.

According to the invention, generally, a microfluidic aliquoting (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. Design improvements including an injection mold design for an insert and a base and a multiplex hole punch allow for rapid fabrication of the MA chip.

An automatic analyzer cartridge, spinnable around a rotational axis, has aliquoting and metering chambers, a connecting duct there between, and a vent connected to the metering chamber and nearer to the rotational axis than the metering chamber. The metering chamber has side walls that taper away from a central region. Capillary action next to the side walls is greater than in the central region. A circular arc about the rotational axis passes through a duct entrance in the aliquoting chamber and a duct exit in the metering chamber. The cartridge has a downstream fluidic element which is part of a fluidic structure for processing a biological sample into the processed biological sample. A valve connects the metering chamber to the fluidic element, which is fluidically connected to the fluidic structure. The fluidic structure receives the biological sample and has a measurement structure for enabling measurement of the processed biological sample.