Disclosed is a nucleic acid detection device which includes an installation unit on which a cartridge is installed, the cartridge including a chamber configured to store nucleic acid, a reagent for amplifying the nucleic acid and a dispersion medium for dispersing the nucleic acid and the reagent; a sample processing unit configured to produce, within the chamber, an emulsion in which droplets containing the nucleic acid and the reagent are dispersed in the dispersion medium; a temperature regulating unit configured to regulate a temperature of the cartridge to amplify the nucleic acid contained in the droplet; and a detection unit configured to detect a signal based on the nucleic acid amplified by temperature regulation by the temperature regulating unit.

Described herein are various embodiments directed to rotor devices, systems, and kits. Embodiments of rotors disclosed herein may be used to characterize one or more analytes of a fluid. A method may include aligning an apparatus to an imaging device. The apparatus may include a set of wells defined by a first layer coupled to a second layer. The first layer may be substantially transparent to infrared radiation. The second layer may define a channel. The second layer may be substantially absorbent to the infrared radiation. The apparatus may further include a third layer coupled to the second layer and define an opening configured to receive a fluid. The third layer may be substantially transparent to the infrared radiation. A set of images of the apparatus may be generated using the imaging device. Bonding information may be generated based on the set of images.

Provided is a centrifugal valve control apparatus including: a body part including a body having a chamber and a channel connected to the chamber, and a valve configured to open and close the channel; a heating part coupled to the body and including a heating member disposed at a position corresponding to the valve; and a rotation driving part configured to rotate the body part and the heating part together, wherein the valve is formed to open and close the channel by the heating member while the body part and the heating part rotate together. Accordingly, the valve of the centrifugal valve control apparatus may be accurately controlled.

A substrate for sample analysis including: a substrate including a rotation axis; a first chamber, which includes a first space which retains the liquid; a second chamber, which includes a second space which retains the liquid discharged from the first chamber; and a first flow passage, which includes a path connecting the first chamber and the second chamber in which the first flow passage has a first opening and a second opening, the first opening and the second opening are connected to the first chamber and the second chamber, respectively, and the first opening is positioned on a side closer to the rotation axis than the second opening, in which the first space includes a first region, which includes a portion extending from the first opening and in which the first space of the first chamber has a capacity larger than a capacity of the first flow passage.

An analysis method irradiates, with laser light, an analysis substrate made of a resin material and having a reaction region on which detection target substances and nanoparticles of a metal compound for labeling the detection target substances are captured. The analysis method extracts, as a substrate signal level, a signal level generated when receiving reflected light from the analysis substrate. The analysis method receives reflected light from the reaction region to generate a light reception level signal. The analysis method extracts a nanoparticle detection signal from the light reception level signal of the reflected light from the reaction region, the nanoparticle detection signal having a higher level than the signal level of the reflected light from the analysis substrate. The analysis method detects the nanoparticles in accordance with the extracted nanoparticle detection signal.

A fluidic module for switching liquid from a liquid retaining area into which liquid can be introduced into downstream fluidic structures includes at least two fluid paths fluidically connecting the liquid retaining area to the downstream fluidic structures. One of the two fluid paths includes a siphon channel. The downstream fluidic structures are not vented or only vented via a vent delay resistor, such that when the liquid is introduced into the liquid retaining area, an enclosed gas volume results in the downstream fluidic structures. By adjusting the ratio of a centrifugal pressure effected by a rotation of the fluidic module and a pneumatic pressure prevailing in the gas volume, the liquid can be retained in the liquid retaining area or can be transferred into the downstream fluidic structures via the siphon channel wherein venting takes place via the other one of the fluid paths.

Sample preparation systems and methods are described having pump control, valve configurations, and control logic that facilitate automatic, inline preparation dilutions of a sample according to at least two dilution operating modes. A system embodiment includes, but is not limited to a first pump configured to drive a carrier fluid; a second pump configured to drive a diluent; and a plurality of selection valves fluidically coupled with the first pump and the second pump, the plurality of selection valves being configured to direct fluid flows from the first pump and the second pump according to at least two modes of operation to provide a single-stage sample dilution according to a first operating mode and to provide a dual-stage sample dilution according to a second operating mode.

According to the invention, generally, a microfluidic aliquot (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. An insert and a base are configured to create an MA chip.

Sample preparation systems and methods are described having pump control, valve configurations, and control logic that facilitate automatic, inline preparation dilutions of a sample according to at least two dilution operating modes. A system embodiment includes, but is not limited to a first pump configured to drive a carrier fluid; a second pump configured to drive a diluent; and a plurality of selection valves fluidically coupled with the first pump and the second pump, the plurality of selection valves being configured to direct fluid flows from the first pump and the second pump according to at least two modes of operation to provide a single-stage sample dilution according to a first operating mode and to provide a dual-stage sample dilution according to a second operating mode.

A device for performing immunoassay tests includes a readable device for performing immunoassay tests having a platform with at least two sample receiving units. Each sample receiving unit is fluidly connectable to at least two test units, each of which includes a reagent incubation chamber fluidly connected to a separation unit connected to a detection chamber. The platform includes a volume indication unit for detecting the presence of a predetermined volume of the liquid biological sample, and a reading device for performing immunoassay tests. A recognition unit recognizes the presence of the readable device and/or extracts information from a legible identification unit of the readable device. A first optical sensor detects the presence of a binding pair of molecules in the separation unit and/or the presence of free molecules in the detection chamber. A second optical sensor detects the presence of a liquid biological sample in the overflow chamber.