A biosensor structure is provided. The biosensor structure includes a substrate, an insulating layer, a semiconductor layer and a gold disc. The insulating layer is disposed on the substrate. The semiconductor layer is disposed on the insulating layer, and a well is disposed in the semiconductor layer. The gold disc is disposed at bottom of the well.

A blocking material is injected into a microfluidic chip that includes microscale-porosity microchannels etched in a substrate, filling at least a portion of the microchannels. Silicon dioxide spheres are injected into the microfluidic chip. The blocking material prevents the silicon dioxide spheres from entering the portion of the microchannels filled with the blocking material. The silicon dioxide spheres form a region of nanoscale porosity in a portion of the microchannels not filled with the blocking material. A solvent is injected into the microfluidic chip, the solvent operable to dissolve the blocking material and thereby providing a region of microscale porosity adjacent to the region of nanoscale porosity.

A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.

A substrate has a plurality of microdots positioned thereon. Each microdot contains one or more primers for gene amplification for a particular target gene. The microdots are placed on the substrate and the substrate is positioned in a housing. The housing has a sample fluid to be tested introduced therein covering the microdot array. While the sample fluid is overlying the substrate, the amplification of the target gene is carried out if it is present within the sample. If the target gene that matches the primers is not present, then amplification will not take place. The fluid also contains fluorophores which will be fixed into the gene as it increases in size as it clearly detects if gene amplification has occurred by detecting the amount of light detected for a particular microdot. In a preferred embodiment, the sample fluid is placed on top of a sealing layer that is less dense then water, such as wax or mineral oil. During a heating of the sample fluid and sealing layer, the sample fluid will sink to the bottom of the sealing layer so that it is fully encased and protected.

Provided is a gene amplification apparatus. The gene amplification apparatus includes a supply roller, a roll-type film chip which has a plurality of polymerase chain reaction (PCR) chambers, with which PCR samples are filled, and is wound around the supply roller, a heating roller configured to rotate after being pressed against the film chip and then induce a PCR, a plurality of heating blocks which are disposed on a circumferential surface of the heating roller at preset intervals and brought into contact with the film chip, and a discarding roller configured to discard the film chip which passes the heating roller and on which the PCR is performed.

An example of an array includes a support including a plurality of discrete wells, a gel material positioned in each of the discrete wells, a sequencing primer grafted to the gel material, and a non-sequencing entity grafted to the gel material. Each of the sequencing primer and the non-sequencing entity is in its as-grafted form.

Methods for performing multiple omics analysis in parallel are provided, the methods can include: dividing the mixture of cells or cell components into at least a first portion and a second portion; performing a first analysis on the first portion to acquire a first set of analytical data; performing a second analysis on the second portion to acquire a second set of analytical data. Methods for forming mixtures of analytes into first and second portions are also provided. The methods can include aligning the first and second plates to engage the first exposed surface with the second exposed surface, wherein the engaging is sufficient to convey at least some of the first analytes into the second solution to form a second mixture of the first analytes.

Methods for forming arrays of droplets, and associated arrays of droplets, are generally provided.

An array microfluidic chip includes a chip mainbody, a transparent hydrophilic membrane, and a covering sheet. The chip mainbody includes a sample loading well and a plurality of reaction wells. The reaction wells are respectively connected to the sample loading well and arranged in an array form. The transparent hydrophilic membrane is disposed on the chip mainbody and covers the reaction wells. The transparent hydrophilic membrane includes a plurality of air pores and a first opening. The air pores are respectively connected to one of the reaction wells. The covering sheet covers the air pores and includes an adhesive element and a vent hole. The covering sheet, the adhesive element and the transparent hydrophilic membrane are stacked to form a vent space.

The disclosure describes an integrated fluid sample test strip comprising: an inlet for receiving solutions comprising a fluid sample and a substrate solution, the inlet comprising a retention valve for temporarily retaining each solution to thereby reduce air flow through the valve; a reaction chamber to receive the solutions via the retention valve, the chamber functionalized with bioreceptor(s); a capillary pump to receive from the reaction chamber the solution(s), the pump comprising vent hole(s); a test chamber to receive the substrate solution from the reaction chamber, the test chamber comprising test electrodes for a biosensing test of the substrate solution; a hydrophobic vent hole coupled to the test chamber to allow a flow of solution from the reaction chamber into the test chamber when the vent hole is unsealed and to allow a flow of solution from the reaction chamber to the capillary pump when the vent hole is sealed.