Sensors having dimensions on the order of nanometers can be arranged in an array. The sensors can detect substances found in an environment. The array of sensors can be disposed on a substrate along with circuitry to control the operation of the array of sensors.

There is provided a microparticle sorting method including a procedure of collecting a microparticle in a fluid that flows through a main channel in a branch channel that is in communication with the main channel by generating a negative pressure in the branch channel. In the procedure, a flow of a fluid is formed that flows toward a side of the main channel from a side of the branch channel at a communication opening between the main channel and the branch channel.

The present disclosure provides methods, systems, and compositions for parallel processing of nucleic acid samples. Methods and systems of the present disclosure comprise the use of sample-specific barcode sequences, which facilitate the multiplexing of samples, detection of discrete cell populations within a pooled population, and detection of partitions comprising more than one cell.

In general the present invention concerns 1) single cell trapping of a viable cell in separate well from a plurality of wells in an array of wells, 2) single cell analysis for the selected cell and 3) single cell lifting of the yet viable cell from the well by an optical tweezer. Furthermore resent invention concerns a cell trap and lift device for B lymphocytes, the device comprising an array of wells in in polymer matrix comprising an off-stoichiometry thiol-ene polymer of the group consisting of off-stoichiometry thiol-ene (OSTE) and off-stoichiometry thiol-ene-epoxy (OSTE+) or a combination thereof that have been grafted with methacrylated polyethylene glycol (methoxy polyethylene glycol methacrylate or (M-PEG-M)) of a number average molecular weight of Mn 2000. It furthermore concerns using the B lymphocyte trap and lift device for trapping single B lymphocyte cells in wells of the device of present invention and lifting said cell from the cell trapping well by optical tweezers, preferably single beam tweezers.

A detection chip and a detection system are provided. The detection chip includes a base substrate, a flow channel defining layer, and at least one driving electrode group. The at least driving electrode group is on the base substrate, and the flow channel defining layer is on a side of the at least one driving electrode group away from the base substrate, the flow channel defining laver includes a flow channel structure, and the flow channel structure is configured to accommodate liquid; and each of the at least one driving electrode group includes a plurality of driving electrodes, and the plurality of driving electrodes are configured to contact the liquid and drive the liquid to move within the flow channel structure.

Methods are used for obtaining, cataloguing, and/or storing data derived from a biological source using a flow cell body, electrodes, and an imaging assembly. The data may include DNA and/or RNA obtained from a biological source, such as from the cells of an organism. The methods may be used to obtain, catalog, and/or store data such as DNA or RNA sequence from a pathogen such as a virus and/or a bacteria, human health data over time, and immune system information from an individual. The data obtained using the disclosed methods may be used for a variety of different purposes, including the manufacture of vaccine compositions, and for restoring the immune system of an individual who has undergone an immune system depleting event. The methods may be used for storage of biological cells, which may be used for the screening of compounds, such as small molecules with potential for therapeutic indications.

A switchable hydrophilic surface is created by attaching electrochemically switchable hydrophilicity polymers to the surface of a microelectrode array. Ferrocene polymers are one example of electrochemically switchable hydrophilicity polymers. Activation of electrodes in the microelectrode array changes the oxidation state of metal ions which switches the polymers between hydrophobic and hydrophilic conformations. Selective activation of electrodes can create patterns of wettability on the microelectrode array that may be varied in real time. The switchable hydrophilic surface may be used to control solid-phase synthesis of polymers. Growing polymers may be selectively extended at locations on the microelectrode array that are hydrophilic. The pattern of hydrophobic and hydrophilic regions can be changed during sequential rounds of synthesis to create a variety of different polymers at different locations on the surface of the microelectrode array.

An automated system communicated to a remote server for diagnostically field testing a sample taken from a patient using an automated portable handheld instrument to determine the presence of Covid-19 and/or antibodies thereto includes microfluidic circuits defined in a rotatable disk for performing a bioassay using a microarray to generate an electrical signal indicative of a bioassay measurement; the microarray operationally positioned in the microfluidic circuit; one or more lasers; one or more positionable valves in the microfluidic circuit; and a backbone unit for rotating the disk according to a protocol to perform the bioassay, for controlling the lasers to selectively open the positionable valves in the microfluidic disk, for operating the microarray to generate a digital image as a bioassay measurement; for communicating the bioassay measurement to the remote server, and for associating the performed bioassay and its corresponding bioassay measurement to the patient.

A thermal block assembly is provided. The assembly can comprise a substrate comprising a first surface configured with a plurality of reaction sites each reaction site configured to contain a biological sample and a sample block comprising a plurality of pedestals configured to thermally modulate the plurality of biological samples wherein each pedestal is thermally coupled to one of the reaction sites. The assembly can further comprise cooling blocks, slots and insulating rings associated with reaction sites each capable of minimizing heat flow between reaction sites. A method for thermally isolating reaction sites is also provided. The method can comprise providing a substrate including a plurality of reaction sites, each reaction site configured to contain a biological sample, providing a sample block comprising pedestals, each pedestal having a dimension substantially equal to a dimension of the reaction site and thermally coupled to the reaction site, thermally isolating the reaction sites with a thermal isolating feature, modulating the temperature of the pedestals through a sequence of temperature and hold times and cooling the reaction sites with cooling blocks.

An analysis unit formed by an analysis body housing an analysis chamber and having a sample inlet and a supply channel configured to fluidically connect the sample inlet to the analysis chamber. Dried assay reagents are arranged in the analysis chamber and are contained in an alveolar mass. For instance, the alveolar mass is a lyophilized mass formed by excipients and by assay-specific reagents.