A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.

Nucleic acid reagents and corresponding methods of using the same for monitoring and evaluating nucleic acid extraction and amplification reactions.

A catcher, a capture device, and a method for capturing at least one target biological particle are provided. The catcher includes a base and a plurality of capture arms extending from the base and spaced apart from each other. Each of the capture arms has a free end portion configured to capture a target biological particle and a supporting segment connected between the free end portion and the base. The supporting segment of each of the capture arms is arranged in a projection space defined by orthogonally projecting the free end portion along a height direction onto the base. When the target biological particle is captured by two of the capture arms that are bent and arranged adjacent to each other, a part of the target biological particle is trapped by the supporting segments of the two of the capture arms and is held.

The present invention relates to a fetal cell capture module, a microfluidic chip for fetal cell capture, and methods for using the same. The fetal cell capture module comprises a cell capture carrier and recognition molecule(s) for specific capture the cell(s). The recognition molecule is attached to the surface of the carrier via an organic conjugate L comprising disulfide bonds. The surface of the chip is modified with recognition molecules that specifically capture fetal cells via organic conjugates comprising disulfide bonds. The recognition molecule, after capturing the cell, achieves the release of the cell by chemically cleaving the disulfide bonds in the organic coupling conjugate. The present invention enables the capture of fetal cell(s) from whole blood without pre-treatment with a high capture rate, low cell loss, simple and accurate cell release operation, and the efficient and noninvasive release of fetal cells and whole genome analysis.

A microscope system includes a microscope stage having a top surface configured to have a sample carrier arranged thereon, the sample carrier being configured to receive at least one sample. The microscope system also includes an imaging system configured to image the at least one sample. The microscope system also includes an injector configured to inject a predetermined amount of a liquid into the sample carrier by injecting multiple successive and temporally spaced jets of the liquid into the sample carrier, each jet including a predetermined portion of the amount of liquid.

A nucleic acid amplification in-situ real-time detection system and method using a micro-fluidic chip through optical fiber sensing. The system includes a white light source, a detection optical path, a microfluidic chip and a spectrum acquisition, processing and display module, which are connected in sequence. The detection optical path is configured to transmit white light from the white light source to the micro-fluidic chip and transmit an optical signal made by the microfluidic chip to the spectrum acquisition, processing and display module. The micro-fluidic chip is configured to carry out biochemical reaction; the spectrum acquisition, processing and display module is configured to acquire the optical signal, analyze the signal and generate a visual biochemical reaction real-time dynamic-change signal curve. This microfluidic chip real-time detection device detects nucleic acid amplification information by using a white light interfered hyperspectral method, so fluorescence-labeled analyte and non-fluorescence-labeled analyte are detected.

A microfluidic system includes a hydrophobic fluidic platform and a heater. The platform includes a plurality of electrode cells operably connected to a voltage source and a controller. The heater is configured to fuse first and second vesicles. The first and second vesicles encapsulate first and second DNA precursors, respectively. The fusing combines the first and second DNA precursors. In another embodiment, a microfluidic system includes a fluidic platform including a plurality of electrode cells, a vesicle mover, and a reaction facilitator. The vesicle mover is configured to move first and second vesicles to a selected cell of the plurality of electrode cells. The reaction facilitator is operably connected to the selected cell. A method includes providing a fluidic platform comprising a plurality of cells; moving first and second vesicles encapsulating first and second reagents, respectively, to a first cell; and fusing the first and second vesicles.

A flow cell includes a flow cell body. The flow cell body includes a frame and a fluid chamber defined in the flow cell body. The fluid chamber includes a reaction region allowing a fluid flow. A liquid inlet, a liquid outlet, and two exhaust holes connected to the fluid chamber are in the frame. Fluid into the liquid inlet flows through the reaction region in the fluid chamber and flows out through the liquid outlet. The exhaust holes discharge gas generated in the fluid chamber during the fluid flow. A flow cell with integral sealing rings and a biochemical substance reaction device are also disclosed.

A device for analysis of cells comprises: an active sensor area (104) presenting a surface for cell growth; a microelectrode array (102) comprising a plurality of pixels (110) in the active sensor area (104), wherein each pixel (110) comprises at least one electrode (120) at the surface, wherein each pixel (110) is configured to control the configuration of the pixel circuitry and set a measurement modality of the pixel; recording circuitry having a plurality of recording channels (130), wherein each pixel (110) is connected to a recording channel (130), wherein each recording channel (130) comprises a reconfigurable component (131), which is selectively controlled between being set to a first mode, in which the reconfigurable component (131) is configured to amplify a received pixel signal, and being set to a second mode, in which the reconfigurable component (131) is configured to selectively pass a frequency band of the received pixel signal.

A detection chip, a using method for the same, and a reaction system. The detection chip includes a first substrate, a micro-cavity defining layer, and a heating electrode. The micro-cavity defining layer is on the first substrate and defines a plurality of micro-reaction chambers. The heating electrode is on the first substrate and is closer to the first substrate than the micro-cavity defining layer, and is configured to heat a plurality of micro-reaction chambers. The orthographic projection of the plurality of micro-reaction chambers on the first substrate is within the orthographic projection of the heating electrode on the first substrate.