An apparatus includes a fluidic coupler including an opening. A first port is in fluid communication with the opening and is to interface with an inlet of a flow cell of a sensor device. A second port is to interface with an outlet of the flow cell of the sensor device. A third port is in fluidic communication with the second port. The apparatus further includes a mechanical assembly moveable between a first position and a second position. The fluidic coupler is secured to the flow cell of the sensor device in the first position. The fluidic coupler is disengaged from the flow cell of the sensor device in the second position.

A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

The present disclosure provides apparatuses, systems, and methods involving a spotter apparatus for depositing a substance from a carrier fluid onto a deposition surface in an ordered array, the spotter apparatus comprising a loading surface including a first well and a second well; and a different outlet surface, including a first opening and a second opening, where a first microconduit fluidly couples the first well with the first opening and a second microconduit fluidly couples the second well with the second opening. An overlay is sealed to the outlet surface and penetrated by a deposition channel that is situated to communicate carrier fluid among the first opening, the second opening, and the deposition surface when the overlay is pressed against the deposition surface.

There is provided a well plate including a plate and a well which is opened in an upper surface of the plate, wherein the well includes a flat bottom surface part and a circumferential wall part rising upward from the circumferential edge of the bottom surface part; the circumferential wall part has a stepped part in the circumferential direction at an arbitrary height position; an upper circumferential wall part, which is located above the stepped part in the circumferential wall part, is larger in a cross sectional area than a lower circumferential wall part located below; and the stepped part indicates the lower limit of the liquid level height of a liquid sample contained in the well.

Methods and containers are provided for identifying a species, illustratively a bacterial species. Illustrative methods comprise amplifying various genes in the nucleic acid from the bacterial species in a single reaction mixture using pairs of outer first-stage primers designed to hybridize to generally conserved regions of the respective genes to generate a plurality of first-stage amplicons, dividing the reaction mixture into a plurality of second-stage reactions, each using a unique pair of second-stage primers, each pair of second-stage primers specific for a target bacterial species or subset of bacterial species, detecting which of the second-stage reactions amplified, and identifying the bacterial species based on second-stage amplification. Methods for determining antibiotic resistance are also provided, such methods also using first-stage primers for amplifying genes known to affect antibiotic resistance a plurality of the second-stage reactions wherein each pair of second-stage primers specific for a specific gene for conferring antibiotic resistance.

The present disclosure describes an apparatus and method of improving temperature uniformity and suppressing well plate warping. In an embodiment, the apparatus includes a barrier configured to be positioned above at least one well configured to contain a liquid sample, where a vessel includes the at least one well, where the vessel is transparent and is configured to be placed within a measurement chamber, where a light measurement apparatus includes the measurement chamber, where the light measurement apparatus is configured to measure light scattered from the liquid sample, where the barrier is configured to seal the at least one well from the measurement chamber, and a weighted lid configured to press a bottom surface of the vessel against a well plate retainer of the measurement chamber, thereby spreading heat among the at least one well and preventing the vessel from warping.

This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.

Devices that includes a first portion, the first portion including at least one fluid channel; a fluid actuator; an analysis sensor disposed within the fluid channel; a conductivity sensor disposed within the fluid channel; and an introducer; a second portion, the second portion comprising: at least one well, the well containing at least one material, wherein one of the first or second portion is moveable with respect to the other, wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel.

A device for performing in situ genetic analyses, conformed so as to be transportable manually by a user, which comprises a casing defining an internal compartment and a plurality of analysis units arranged in the internal compartment, where each analysis unit is configured to perform a respective and independent genetic analysis of at least one sample; each analysis unit comprises a sample holder compartment accessible by the user and adapted to accommodate at least one sample; a command and control unit; at least one sensor selected among an optical, acceleration, temperature, pressure, motion, chemical sensor or a combination thereof, configured to detect a first physical quantity relative to the genetic analysis of the at least one sample and to transduce the first physical quantity into a first signal which is indicative of the state of progress of the genetic analysis, the command and control unit is in signal communication with the at least one sensor for receiving said first signal; the analysis unit further comprises a plurality of instruments, configured to perform the genetic analysis of the sample, comprising an amplification and optical detection device configured to detect at least a second physical quantity relative to the genetic analysis and to transduce the second physical quantity into at least a second signal which is indicative of the outcome of the genetic analysis, the command and control unit is in signal communication with the amplification and optical detection device for receiving said second signal; the device further comprises a processing unit, in signal communication with the command and control unit of each analysis unit for receiving the respective first signals and the respective second signals.

Techniques are described for dynamically correcting image distortion during imaging of a patterned sample having repeating spots. Different sets of image distortion correction coefficients may be calculated for different regions of a sample during a first imaging cycle of a multicycle imaging run and subsequently applied in real time to image data generated during subsequent cycles. In one implementation, image distortion correction coefficients may be calculated for an image of a patterned sample having repeated spots by: estimating an affine transform of the image; sharpening the image; and iteratively searching for an optimal set of distortion correction coefficients for the sharpened image, where iteratively searching for the optimal set of distortion correction coefficients for the sharpened image includes calculating a mean chastity for spot locations in the image, and where the estimated affine transform is applied during each iteration of the search.