Instrument free “plasticware” is provided that enables multi-step molecular reactions in a diagnostics context. A hollow plunger that is movable within a surrounding tube defines a reaction chamber inside the plunger. By moving the plunger to different positions in the tube, a sample can be collected, then the sample can be washed, and finally the reaction chamber can be sealed to perform diagnostic reactions on the sample. The juxtaposition of large sample collection/washing volume with small reaction volume allows one to conduct a wide range of diagnostic assays including a LAMP (Loop mediated isothermal amplification) based saliva test in a small, portable self-contained device. Applications include Molecular diagnostics for health applications (including COVID19 test), Environmental Monitoring, Disease surveillance, and Veterinary health.

A microfluidic analysis device and manufacturing method are provided. The microfluidic analysis device includes a capillary substrate, a cover substrate adjacent to a cover side of the capillary substrate and/or a bottom substrate adjacent to a bottom side of the capillary substrate, a capillary structure with at least one capillary, forming a hollow channel, in the interior of the capillary substrate and/or at the interface of the capillary substrate with the cover substrate and/or at the interface of the capillary substrate with the bottom substrate and also a fluid-conducting arrangement for conducting a fluid through the capillary structure. The fluid-conducting arrangement may be designed for compartmenting the fluid by way of controlled pressure pulses. A linear sensor element, which extends toward a capillary of the capillary structure and/or away from it and/or along the capillary, and a fluid contact end of which and at least an adjacent part of its feed lie in an identical plane to the capillary, may be integrated in the microfluidic analysis device, the element finishing with its fluid contact end flush against a side wall of the capillary or extending into the hollow channel thereof.

A biological fluid collection device that receives a sample and provides flow-through blood stabilization technology and a precise sample dispensing function for point-of-care and near patient testing applications is disclosed. A biological fluid collection device of the present disclosure is able to effectuate distributed mixing of a sample stabilizer within a blood sample and dispense the stabilized sample in a controlled manner. In this manner, a biological fluid collection device of the present disclosure enables blood micro-sample management, e.g., passive mixing with a sample stabilizer and controlled dispensing, for point-of-care and near patient testing applications.

The present application is generally directed to systems, methods, and devices for diagnostics for sensing and/or identifying pathogens, genomic materials, proteins, and/or other small molecules or biomarkers. In some implementations, a small footprint low cost device provides rapid and robust sensing and identification. Such a device may utilize microfluidics, biochemistry, and electronics to detect one or more targets at once in the field and closer to or at the point of care. In some implementations, the systems and methods herein implement a reader device, an assay cartridge, and a mobile or external device configured to receive abiological sample, test the biological sample, and provide test results to a patient or user associated with the patient. The test results may be packaged with additional information, including symptoms suffered by the patient, a diagnosis, and follow-up instructions. In some embodiments, the test results may also be provided with or aggregated with other test results to generate aggregate information.

Unitary measuring pipettes include a tubular body of biaxially oriented thermoplastic material, together with size reduction, elimination, and/or reorientation of longitudinally spaced, raised circumferential witness features, to mitigate or avoid interference between such witness features and graduated volumetric markings on an outside surface of the tubular body. Methods and apparatus for vacuum forming of unitary measuring pipettes are also provided. Gas permeable apertures or pores having a maximum width of no greater than 150 microns, in ranges of 10-100 microns, 10-50 microns, or subranges thereof, may be defined in face plates or inserts received by mold blanks, or defined in molding surface of cooperating mold bodies, and may be used to produce a tubular pipette body having reduced height witness features. Cooperating mold bodies may each be produced from multiple mold body sections with gas passages defined therein and/or therebetween.

The present invention relates generally to methods and materials pertaining to assays, for example immunoassays, for biomarkers in body fluids e.g. blood. The invention also relates to diagnostic or screening methods for infections, and methods of differentiating between infectious and non-infectious conditions in mammals, particularly equines, for monitoring response to anti-infective/antibiotic therapy. The invention further relates to a test fluid collection system adapted to permit dilution and analysis of the collected test fluid. The invention further relates to monitoring exertional rhabdomyolysis in equines, and assay devices for all these things.

A dispenser and methods for transferring liquids are disclosed. The dispenser may include a capillary tube with tip having an aperture, a piezoelectric actuator coupled to the capillary tube at a location. Actuation of the piezoelectric actuator causes a pressure wave to propagate along the capillary tube toward the tip such that radial motion at the location is transmitted as distally extending axial motion of the tip, thereby causing a droplet of a predetermined volume to be ejected from the aperture. In some embodiments, the capillary tube has a modulus of elasticity in a range which dampens acoustical noise from the actuation and provides single drop stability over a range of drop sizes.

Provided is a technique for moving all of a droplet from a microchannel in which the droplet have been introduced to another layer.

The droplet transport device of the present disclosure includes a substrate having a through-hole or a recess, a first electrode provided on the substrate along the surface of the substrate and arranged at a position adjacent to the through-hole or the recess, a plurality of second electrodes provided on the substrate along a surface of the substrate and to which a voltage for moving the droplet introduced on the substrate is applied, and a dielectric layer covering the surface of the substrate, the first electrode, and the second electrodes, and a water-repellent film provided on the inner wall surface of the through-hole or the recess, and on the dielectric layer.

Disclosed herein are carbon nanotube arrays as well as transfer systems comprising said carbon nanotube arrays and an administration platform. The disclosed carbon nanotube arrays can also be provided in kits further comprising a culture platform. Also disclosed herein is the use of said carbon nanotube arrays and transfer systems in administering agents to a cell.

Systems and methods are disclosed that utilize metal nanostructures that are synthesized in situ along the internal surfaces of a microfluidic device. The nanostructures are formed by initial deposition of metallic seeds followed by flowing growth and reducing agent solutions into the capillaries/microfluidic channels to grow the nanostars. The nanostructures may optionally be functionalized with a capture ligand. The capture ligand may be used to selectively bind to certain cells (e.g., circulating tumor cells). The cells may be removed by a beam of light (e.g., laser beam) that induces localized heating at the surface location(s) containing the nanostructures. The plasmonic nature of the nanostructures can be used to heat the nanostructure(s) locally for the selective removal of one or certain cells. The nanostructures may be used to acquire Raman spectra of molecules or other small objects that are bound thereto for identification and quantification.