A method of analyzing a sample comprising one or more species of microorganisms can include generating first droplets such that each of one or more microorganisms of a first portion of the sample is encapsulated within one of the first droplets and, for each of one or more aliquots of a second portion of the sample, second droplets such that each of one or more microorganisms of the aliquot is encapsulated within one of the second droplets. First and second sets of data can be captured, the first set indicative of the identity and quantity of encapsulated microorganism(s) of the first portion of the sample and the second set indicative of a phenotypic response of encapsulated microorganism(s) of the aliquot(s) to one or more test reagents. A target species' phenotypic response to the test reagent(s) is determinable at least by referencing the second data set to the first data set.

The present disclosure relates to a computer aided design (CAD) manufactured lattice structure. The structure may have a plurality of tessellated cells formed from a plurality of interconnected struts, with the interconnected struts formed from a curable resin. The interconnecting struts form voids within each cell, with the voids communicating with one another. The struts may be formed such that the voids have a non-uniform dimension to create a varying porosity within the lattice structure.

An apparatus includes a flow cell interface adapted to be coupled to a flow cell having a plurality of channels and a pump manifold assembly carrying pump valves and pumps and including pump-channel fluidic lines, pump fluidic lines, and a shared fluidic line. The pump valves and the pumps are operable to individually control fluid flow through each channel of the plurality of channels of the flow cell via the corresponding pump-channel fluidic lines. Each pump valve being coupled to a corresponding pump-channel fluidic line, a corresponding pump fluidic line, and the shared fluidic line and being movable between a first position fluidically coupling a corresponding channel, a corresponding pump-channel fluidic line, and a corresponding pump fluidic line and a second position fluidically coupling a corresponding pump fluidic line, the shared fluidic line, and a waste reservoir. Each pump coupled to a corresponding pump fluidic line.

A method of fabricating a nanoscale topography system for inducing unfolding of a DNA molecule for sequencing includes providing a substrate and creating trench walls on the substrate which define a trench therebetween. The method further includes depositing a layer of a block copolymer (BCP) in the trench and forming cylindrical domains by self-assembly of the BCP between the trench walls, removing a first portion of the cylindrical domains to create a vacant region in the trench, and depositing a subsequent layer of the BCP in the vacant region and forming spherical domains by self-assembly of the BCP between the trench walls adjacent a second portion of the cylindrical domains. The spherical domains form staggered post structures for unfolding the DNA molecule and the cylindrical domains form parallel channel structures for entry of the DNA molecule for sequencing.

A blood sample collection and/or storage device includes a medium, such as a membrane or microstructured environment for storing a body fluid sample such as a blood sample. The medium has hydrophobic patterns formed thereon or therein to define precisely dimensioned channels for fluid flow or fluid retention. Break lines in the medium defined predetermined areas (or volumes) of the medium. After sample collection, the medium may be broken apart along the break lines to obtain a precisely measured amount of the fluid sample.

Exemplary liquid lenses generally include two liquids disposed within a microfluidic cavity disposed between a first window and a second window. Applying varying electric fields to these liquid lenses can vary the wettability of one of the liquids with respect to this microfluidic cavity, thereby varying the shape and/or the curvature of the meniscuses formed between the two liquids and, thus, changing the optical focal length or the optical power of the liquid lenses. These liquids can expand and/or contract as result of varying temperatures. The exemplary liquid lenses include one or more thermal compensation chambers to allow these liquids to expand and/or contract without impacting the integrity of the microfluidic cavity, for example, without bowing or deflecting the first window and/or the second window.

The present disclosure provides devices, kits, and methods for enriching/separating and/or subtyping target cells from a biological sample, such as circulating tumor cells or other types of rare cells or differentiating cells. Devices, kits, and methods of the present disclosure utilize a created chemogradient to modulate movement of target and/or non-target cells in a sample based on attraction and/or repulsion to certain chemical compounds to separate and subtype cells in a sample.

Embodiments of the disclosure include methods and apparatuses for separating beads from a droplet main body on a microfluidics actuator by applying a magnetic field to a droplet disposed at a first location, the droplet including one or more magnetically responsive beads; and moving the magnetic field to separate the one or more magnetically responsive beads from a main body of the droplet. Embodiments also include methods and apparatuses for introducing one or more beads into a droplet main body by applying a magnetic field to one or more magnetically responsive beads and moving the magnetic field to introduce the one or more magnetically responsive beads into a droplet disposed on a first location, wherein the droplet includes a fluid.

A cell sorting device is disclosed, wherein the device includes a magnetic separation debulking stage followed a microfabricated fluid valve. The magnetic separation stage may use a sorting magnet and sort particle using magnetic beads attached to the particles. The device may include at least one fluid channel, wherein the sorting magnet and the particle manipulation device are in fluid communication with one another through at least one fluid channel. A method of sorting cells from a first cell suspension is also disclosed, The method may include a) magnetic labeling of first target cells and removal of the non-target cells by applying magnetic fields to obtain a second cell suspension; b) fluorescence-activated labeling of second target cells present in the second cell suspension and separating the fluorescence-activated second target cells from the not labeled cells to obtain a third cell suspension.

An apparatus includes one or more valves adapted to be coupled to corresponding reagent reservoirs and a flow cell interface adapted to be coupled to a flow cell. The apparatus includes a sample cartridge interface having one or more ports and adapted to be coupled to a sample cartridge carrying a sample of interest. The sample cartridge interface positioned downstream of the flow cell interface. The apparatus includes a pump adapted to load a channel of the flow cell with the sample of interest via the flow cell interface associated with an outlet of the flow cell and a corresponding port of the sample cartridge interface.