The present invention discloses a multi-flux microfluidic chip for nucleic acid detection and capable of actively controlling a flow path, and a use method thereof. The detection chip includes a chip body, and the chip body is provided with a sample loading chamber, a reaction chamber, and a microfluidic channel, where there is a plurality of reaction chambers, and the microfluidic channel includes a sample output main channel and several sample distribution channels. The sample distribution channels are separately disposed, and include sample distribution chamber sample output channels and reaction chamber sample input channels, where the sample distribution chamber sample output channels and the reaction chamber sample input channels can be communicated through sample distribution connection channels formed through heat sealing processing for a thin film on the chip body, and a thin film part of the sample distribution connection channel can form a sample distribution occlusion portion after undergoing heat sealing processing, to intercept the flow in the sample distribution channels. Therefore, the present invention uses heat sealing technology to replace a micro valve to control opening/closing of a channel, so as to decrease the number of parts mounted on the microfluidic chip, and reduce the continuous work of an instrument and energy consumption thereof. Moreover, the present invention achieves a multi-flux detection effect.

Systems, methods, and devices for discretizing and analyzing fluidic samples are provided. In one aspect, a microfluidic array for discretizing a fluidic sample comprises one or more flow channels and a plurality of fluidic compartments in fluidic communication with the one or more flow channels. In another aspect, a system for discretizing and analyzing fluidic samples comprises a rotor assembly shaped to receive a microfluidic device.

The present invention describes a device(s) and assay(s) for the isolation and analysis of individual components from a sample. The invention provides a means of both isolating a multitude of individual components into an organized array and the subsequent analysis of such components by various detection and analysis methodologies. The invention provides a significant advancement in both the number of individual components that can be individually analyzed as well as enabling the quality and number of analytical methodologies that can be applied to them.

A biological fluid sampling transfer device adapted to receive a multi-component blood sample is disclosed. After collecting the blood sample, the biological fluid sampling transfer device is able to separate a plasma portion from a cellular portion. After separation, the biological fluid sampling transfer device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid sampling transfer device also provides a closed sampling and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with a sample stabilizer. The biological fluid sampling transfer device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the biological fluid sampling transfer device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results.

Devices and methods for analyzing a sample are disclosed. In various embodiments, the present disclosure provides devices and methods for preparing a serial dilution of a sample. In various embodiments, the present disclosure provides devices and methods for preparing a serial dilution of a sample and conducting sample analysis. In various embodiments, the present disclosure provides a cartridge device and a reader instrument device. The reader instrument device receives, operates, and/or actuates the cartridge device to prepare a serial dilution of a sample and conduct sample analysis.

Provided is a fluid analysis apparatus and a method of controlling the same. The fluid analysis apparatus include an actuator provided on a part of the fluid analysis apparatus, a mounting portion on which a fluid accommodating cartridge is mounted thereon, the fluid accommodating cartridge provided with a well in which a fluid sample is accommodated, a measurement portion configured to transmit light to the fluid accommodating cartridge and detect an optical signal from the light passed through the fluid accommodating cartridge, and a controller configured to control an operation of the actuator based on the optical signal detected by the measurement portion such that the light transmitted from the measurement portion passes through a central portion of the well to perform an accurate inspection on the fluid sample.

The present invention is directed to the use of microfluidics in the preparation of cells and compositions for therapeutic uses.

The present invention relates to a high-speed screening and analysis system for reaction optimization. More specifically, the present invention provides a system capable of analyzing samples at low cost through control of fluids using hydrophilic plate-like material (for example, paper), and of analyzing chemical reactions of a sample with a plurality of materials simultaneously, thereby allowing samples to be analyzed rapidly.

Provided herein are methods of splitting droplets containing magnetically responsive beads in a droplet actuator. A droplet actuator having a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at the droplet operations electrodes, is provided. The magnetically responsive beads in the droplet are immobilized using the magnetic field and the plurality of droplet operations electrodes are used to split the droplet into first and second droplets while the magnetically responsive beads remain substantially immobilized.

A microfluidic device includes an inlet port configured to receive a sample, a first reaction chamber fluidically coupled to the inlet port, a first pump fluidically coupled to the inlet port, a second pump fluidically coupled to a mixing chamber, a metering channel fluidically coupled to the first reaction chamber and to the mixing chamber, and one or more second reaction chambers fluidically coupled to the mixing chamber. The first pump is configured to move fluid from the inlet port to the first reaction chamber and from the first pump to the inlet port. The second pump is configured to move fluid from the second pump to the mixing chamber, from the first reaction chamber to the mixing chamber, and from the mixing chamber to the one or more second reaction chambers.