A fluid handling device (100) has a case (110) and a containing section (120). The containing section (120) includes a side wall formed in a substantially circular cylindrical shape, a plurality of chambers, and a plurality of communication holes. The inner peripheral surface (131) of the case (110) includes: a plurality of divided inner peripheral surfaces (132) which surround a rotation axis (RA) and which each slope toward the rotation axis (RA) as the divided inner peripheral surface (132) extends toward the bottom of the case (110); and a step surface (133) disposed between two adjacent divided inner peripheral surfaces. At least part of the outer peripheral surface of the containing section (120) is in contact with the plurality of divided inner peripheral surfaces (132) of the case.

Disclosed herein are methods for performing assays, including general functional assays, on a biological cell. The methods can include contacting a biological cell with a test agent for a period of time; lysing the biological cell while the biological cell is disposed within a sequestration pen located within an enclosure of a microfluidic device; and allowing RNA molecules released from the lysed biological cell to be captured by capture oligonucleotides linked to a capture object disposed within the sequestration pen of the microfluidic device. Each capture oligonucleotide can include a priming sequence that binds a primer, and a capture sequence. Each cDNA transcribed from a captured RNA can have an oligonucleotide sequence complementary to the captured RNA molecule, with the complementary oligonucleotide sequence being covalently linked to one of the capture oligonucleotides of the capture object.

A fluidic chip employing a vacuum void to store vacuum potential for controlled micro-fluidic pumping in conjunction with biomimetic vacuum lungs.

The present disclosure provides systems, methods, and devices for processing a biological sample. The device may be a microfluidic device comprising a fluid flow path and a chamber. The fluid flow path may comprise a channel and an inlet port and no outlet port. The inlet port may be configured to direct a biological sample to the channel. The channel may be in fluid communication with the chamber. The chamber may be configured to receive a portion of the biological sample from the channel and retain the biological sample during processing.

The present invention relates to a microfluidic device and method for providing emulsion droplets. The device comprising: a microfluidic section comprising one or more microfluidic units; and a well section comprising one or more groups of wells comprising one group of wells for each microfluidic unit; the well section and the microfluidic section forming a fixedly connected unit such that each group of wells forms a fixedly connected unit with a respective corresponding microfluidic unit, each microfluidic unit comprising a fluid conduit network comprising: a plurality of supply conduits comprising a secondary supply conduit and a primary supply conduit comprising a capillary structure having a volume of at least 2 L; a transfer conduit; and a first fluid junction providing fluid communication between the primary supply conduit, the secondary supply conduit, and the transfer conduit; each group of wells comprising a plurality of wells comprising a collection well and one or more supply wells comprising a primary supply well, the collection well being in fluid communication with the transfer conduit of the corresponding microfluidic unit, the primary supply well being in fluid communication with the primary supply conduit and the secondary supply conduit of the corresponding microfluidic unit.

A microfluidic device includes a lower casing and an upper casing covering the lower casing. The lower casing includes a lower base wall having a top surface and a plurality of spaced-apart columns that protrude upwards from the top surface. The upper casing includes an upper base wall. A first gap between the upper base wall and a column top surface of each of the columns is large enough to permit passage of large biological particles of a liquid sample, and a second gap between any two adjacent ones of the columns is not large enough to permit passage of the large biological particles and is large enough to permit passage of small biological particles of the liquid sample.

The present invention relates to an analyte detection device comprising: a sample chamber for storing a mixture solution of a sample comprising an analyte and a reactant comprising particles; a detection chamber for storing a detection solution; and a channel placed between the sample chamber and the detection chamber to prevent the mixture solution and the detection solution from being mixed with each other, the analyte detection device characterized by detecting the analyte by moving the particles from the sample chamber to the detection chamber using moving means.

Examples include polymerase chain reaction (PCR) devices. Example PCR devices comprise a fluid input, a fluid output, and a set of microfluidic channels that fluidly connect the fluid input and the fluid output. Each microfluidic channel comprises a reaction chamber, and examples further comprise at least one heating element, where the at least one heating element is positioned in the reaction chamber of each microfluidic channel. The at least one heating element is to heat fluid in the reaction chamber of each fluid channel, and the at least one heating element is to pump fluid to the reaction chamber and from the reaction chamber of each microfluidic channel.

Provided herein according to some embodiments is an in vitro construct useful as a model for a hematopoietic microenvironment, which may include: a microfluidic device having multiple chambers; and two or more populations of cells (e.g., 3 or 4 populations of cells) (or niches) selected from: 1) mesenchymal cells (e.g., Stro-1+; MSC); 2) osteoblasts (OB; optionally said osteoblasts provided by differentiating mesenchymal cells to differentiated osteoblasts); 3) arterial endothelium (e.g., CD146+NG2+; AEC); and 4) sinusoidal endothelium (CD146+NG2; SEC), wherein each of said two or more populations of cells are provided in a separate chamber of the microfluidic device. Methods of making and using the construct are also provided.

A multiplexed lateral flow device includes an impermeable internal reservoir having an opening to receive a sample deposition. A fluid distributor pad is arranged in fluid communication with a lower surface of the internal reservoir. The fluid distributor pad includes a paper based microfluidic element having a pattern of a hydrophobic material to distribute a portion of the sample deposition substantially equally among a plurality of flow paths. Lateral flow assays having a plurality of flow lines are aligned with flow paths of the distributor pad. An impermeable top cover has a first window arranged over the opening of the internal reservoir, and at least a second window arranged over the test results of the lateral flow assays. A housing element houses the reservoir, the distributor pad and lateral flow assays. The housing element includes an impermeable bottom cover and a spacer element arranged between the top and bottom covers and, provides a gap between the lateral flow assays and the impermeable top cover.