A collection device for a biological sample to capture target compounds such as viruses or other pathogens or particles for testing from within the sample and move the captured target compound to a separate chamber for subsequent processing. The collection device can include an openable substance blister including capture particles located in a cup interior. Capture particles can attract and bind the target compounds from the sample. An extraction tube extracts any nucleic acid from the target compound for storage or subsequent amplification and testing to confirm presence of known microorganisms. The extraction tube can comprise a heat-deformable material and can be connected to a microfluidic cartridge for further processing of nucleic acid including, amplification and detection. The microfluidic cartridge includes valves and a plurality of chambers for amplification.

The present invention is directed to a recirculation system for use in microfluidic centrifugal disc platforms for reusing and mixing an entire sample. The present invention features a system comprising a reservoir, an input channel, a detection array, a pressure chamber, and a recirculation channel connecting the pressure chamber to the reservoir. The recirculation channel may have a resistance lower than the channel upstream resistance. When the CD platform spins at a high RPM, the liquid may be directed from the reservoir into the pressure chamber. When the RPM of the CD platform decreases rapidly, the liquid may be from the pressure chamber through the channel and through the recirculation channel to the reservoir, such that the liquid travels through the recirculation channel faster than the liquid travels through the channel.

Intermittent warming of a biologic sample including a nucleic acid includes receiving at a first end of a channel of a microfluidic device, a biologic sample including a nucleic acid, and warming a subset of a plurality of heating elements disposed adjacent to the channel. The method includes warming the heating elements to a particular temperature of a particular warming and cooling protocol. The method includes moving the biologic sample from the first end of the channel to a second end of the channel opposite the first end at a particular flow rate associated with the warming and cooling protocol, and intermittently warming the biologic sample using the subset of heating elements while the biologic sample moves from the first end of the channel to the second end of the channel.

The disclosure describes methods and devices with which to process and analyze difficult chemical, biological, environmental samples including but not limited to those containing bulk solids or particulates. The disclosure includes a cartridge which contains a separation tube as well as one or more valves and cavities for receiving raw sample materials and for directing and containing various fluids or samples. The cartridge may contain a separation fluid or density medium of defined density, and structures which direct particulates toward defined regions of the cartridge. Embodiments can include a rotational device for rotating the cartridge at defined rotational rates for defined time intervals. Embodiments allowing multiple assays from a single sample are also disclosed. In some embodiments, this device is used for direct processing and chemical analysis of food, soil, blood, stool, motor oil, semen, and other samples of interest.

The present disclosure relates to devices and systems for separating motile pathogenic bacterial cells from samples. The disclosure also provides for methods of determining whether a sample is contaminated by pathogenic bacteria. The devices and systems disclosed herein are useful for screening water sources, environmental testing sites, food sources, and bodily fluids for the presence, absence, or quantity of bacterial cells in a sample representative of the screened sources.

The invention provides a device for detecting an analyte in a liquid sample. The device comprises a receiving chamber for receiving an absorbent element, wherein the absorbent element is used for collecting a liquid sample; and a detection chamber configured to receive a testing element, wherein the testing element is set to test an analyte in a liquid sample, the receiving chamber is provided with a first position and a second position, when the receiving chamber is located at the first position, the receiving chamber is not in fluid communication with the detection chamber, and when the receiving chamber is located at the second position, the receiving chamber is in fluid communication with the detection chamber.

The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.

A system for filling multiple sterile containers includes a filter with an inlet port and multiple outlet ports, the outlet ports being pre-attached to sterile containers by respective filling lines of each container. Each container has an interior and each of the respective filling lines are connected to a respective container interior. The respective filling lines are sealed to the outlet ports and the containers such that the container interiors are isolated from an external environment except the inlet port, via the filter, forming a combined interior volume which is sterile. A container that is connectable to an outlet port the system has a bladder, a first tube and a second tube connected to the bladder, and a sterilizing filter. The container, the first tube and the second tube, and the sterilizing filter are sterile before water is flowed through the sterilizing filter into the bladder.

A method and microfluidic device to perform reservoir simulations using pressure-volume-temperature (“PVT”) analysis of wellbore fluids.