A method and an analysis system for testing a biological sample, nucleic-acid products being hybridized to capture molecules of a sensor apparatus at different temperatures.

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

This disclosure provides devices and methods for the isolation of single cells or particles of interest from a solution comprising a plurality of cells or a solution composed of a homogenous population of particles. Specifically, the present disclosure is directed to microfluidic devices and methods for analyzing cells in a sample. More specifically, the present disclosure provides droplet microfluidic devices and methods for using the same to obtain (trap), encapsulate, and retrieve (isolate) single cells or particles from a sample with improved efficiency.

A multi-channel bio-separation system configured to utilize a cartridge that has a individual, separate integrated reagent (i.e., a separation buffer) reservoir dedicated for each separation channel. The multiple channels may have different characteristics, such as different separation medium of different chemistries, different separation length, different channel sizes and internal coatings. In one embodiment, the cartridge does not include integrated detection optics. Not all channels need to be operative. One or more of the channels in the cartridge may be dummy channels that are not operative (e.g., not provided with a capillary tube). A capillary tube may be routed between the reservoir/electrode (anode) of one channel to an electrode (cathode) in another channel, thus allowing a longer length of capillary tube to be used to define a longer separation channel to improve resolution.

A sample processing kit including a centrifugal microfluidic component and a collection component detachably fitted into the microfluidic component is provided. Upon the application of the sample processing kit, target molecules or cells may be separated and collected by the collection component for further experimentation.

A micro-fluidic chip comprises a chip base, a lens, and a securing portion. The chip base has a flow cell and a micro-fluidic channel defined therein. The micro-fluidic channel is fluidly connected to the flow cell to deliver fluid to and from the flow cell, respectively via a fluid input port and a fluid output port. The lens has an apex and a base. The apex is positioned within the flow cell. The securing portion is affixed to the chip base such that the lens is sandwiched between the chip base and the securing portion. The securing portion has a circular cavity defined therein in a surface adjacent the chip base, for receiving the base of the lens. The securing portion further has separate light input and output channels to allow light in and out, respectively, of the circular cavity and the lens.

A fluid loader is provided for loading fluid into a microfluidic device, the microfluidic device having upper and lower spaced apart substrates defining a fluid chamber therebetween and an aperture for receiving fluid into the fluid chamber. The fluid loader includes a fluid well communicating with a fluid exit provided in a base of the fluid loader. The base of the fluid loader is shaped, in use, to locate the fluid loader relative to the aperture, and to direct fluid leaving the fluid loader via the fluid exit preferentially in a first direction in the fluid chamber of the microfluidic device. In one embodiment the base of the fluid loader includes a protruding portion having at least first and second legs, the first leg being shorter than the second leg. In use, the fluid loader is positioned such that the first leg of the fluid loader is between a fluid loading area associated with the aperture and an operating area of the device.

A trade metal analysis detector and method of operating the same to detect metals in various fluid samples using boron doped diamond working electrodes.

Disclosed herein is a system that relies upon an assay card employing only capillary action and no outside energy source containing cargo-loaded gated porous nanoparticles, an optional device cartridge, an optional reagent container, and optional software for detection and/or quantitation of at least one target analyte of a liquid sample.

Method of detecting a target nucleic acid. In an exemplary method, at least two thermal zones of different temperature may be created using a heating assembly. A first emulsion and a second emulsion may be formed. The first and second emulsions may be thermally cycled by passing them through tubing in a spaced relation to one another, with the tubing being wound around a central axis of the heating assembly and extending through each thermal zone multiple times. Thermally cycling may promote amplification of the target nucleic acid in droplets of each emulsion. Droplets of each emulsion may be passed through a detection channel located downstream of the tubing. Fluorescence may be detected from the droplets being passed through the detection channel.