Described herein are various embodiments directed to rotor devices, systems, and kits. Embodiments of rotors disclosed herein may be used to characterize one or more analytes of a fluid. An apparatus may include a first layer being substantially transparent. A second layer may be coupled to the first layer. The second layer may be substantially absorbent to infrared radiation. The second layer and the first layer may collectively define a set of wells. The first layer may define a base for each well of the set of wells. The second layer may define an opening for each well of the set of wells. At least one of the first layer and the second layer may define a sidewall for each well of the set of wells.

Embodiments of the invention are directed to a method of separating and encapsulating an analyte on a microfluidic device in order to extract the analyte. A microfluidic device is provided having a main microchannel and a set of one or more auxiliary microchannels, each branching to the main microchannel at respective junctions therewith. A mixture is introduced as a single phase in the main microchannel in order to electrokinetically separate an analyte from the introduced mixture, and in order to confine the separated analyte in a microchannel portion of the main microchannel. The microchannel portion adjoins one of the junctions. One or more encapsulating volumes of an encapsulating phase are injected in the main microchannel via one or more of the auxiliary microchannels. The encapsulating phase is immiscible with said single phase. The encapsulated analyte is extracted from the main microchannel via one or more of the auxiliary microchannels.

A device for separating blood plasma from whole blood includes a first reservoir and a second reservoir. The first reservoir is configured to receive a sample of whole blood including red blood cells and includes a collection region and a constricted region. The second reservoir is fluidically connected to the constricted region of the first reservoir, such that, responsive to centrifugal force applied to the device, the sample of whole blood disposed within the first reservoir separates into a first fraction and a second fraction. The first fraction is located in the collection region and includes blood plasma from which substantially all red blood cells have been removed. The second fraction is located in the second reservoir and includes blood plasma and red blood cells that have been removed from the first fraction by the centrifugal force. The constricted region inhibits the second fraction from entering the collection region.

A specimen delivery cartridge includes a lower housing, and an upper housing. The upper housing is coupled to the lower housing at a hinge. The specimen delivery cartridge further comprises a testing chamber comprising a paper testing substrate. The paper testing substrate may include a wicking conduit and a plurality of test areas. The specimen delivery cartridge may also include a lens assembly proximate the plurality of test areas and operable to transmit light emissions from the plurality of test areas to an image sensor of a computing device. In some embodiments, the specimen delivery cartridge includes a testing substrate having a plurality of test areas made of an array of electrodes. Each electrode is printed on a first side of the testing substrate and coupled to a conductive via formed in the testing substrate and a conductive trace printed on a second, opposing side of the testing substrate.

A sample holder for use in a centrifuge, the sample holder being generally planar and comprising: an aperture or recess for releasably retaining a sample storage member including a sample chamber adapted to contain a volume of liquid; a centre point around which the holder will rotate during use; and one or more calibration features, wherein the calibration feature(s) comprise one or more outer edges, which lie on the side of the or each calibration feature which is furthest from the centre point, and the one or more outer edges comprise a series of radially spaced-apart outer edge portions or positions which are spaced at different distances from the centre point as a function of angular position around the centre point.

The present invention is directed to the use of microfluidics in the preparation of cells and compositions for therapeutic uses.

A mechanically-actuated vacuum-controlled fluid collection system includes a mechanically-actuated vacuum controller (MA VC) to draw fluid into a chamber through the opening to the chamber. The system may include a releasable seal to seal the opening, and the MAVC may include a spring-loaded plunger to create a vacuum within the chamber when sealed. The system includes multiple fluid chambers, and may further include a single actuator or multiple corresponding actuators. The system may be configured to add a pre-loadable reagent to fluid drawn into the one or more chambers, and may be configured to add the reagent in proportion to a volume of the fluid. The system may be controllable to release collected fluid to another device, such as for assay and/or transport. The system may be configured to draw a liquid biological sample such as urine, and may include a fluid interface to draw fluid from a biological sample container.

A centripetal microfluidic platform comprised of a microfluidics disc and a reader for testing LAL-reactive substances in fluid samples is provided. The microfluidic disc may comprise at least two testing areas wherein each testing area includes a reservoir portion for receiving at least one fluid sample. The disc may comprise a distribution network portion in fluid communication with the reservoir portion. Each distribution network portion may comprise a distribution network of at least four (4) channels, wherein each channel has a metering portion and at least one analysis chamber portion. The analysis chamber portion may comprise a mixing chamber for mixing samples and reagents and an optical chamber portion that is compatible with an optical reader. The metering portion may be sized to meter an aliquot of the fluid sample for analysis in the analysis chamber portion. At least one analysis chamber portion has at least one reagent isolated therein. The centripetal microfluidic platform further includes a reader for testing fluid samples within a microfluidic disc comprising an enclosure, an optical bench, a centripetal disc drive, and a controller. A method for testing at least one fluid sample for LAL-reactive substances is also provided.

A microfluidic device includes a first substrate including at least one microfluidic channel and a plurality of microwells, as well as a cooperating second substrate defining multiple split-walled cell trap structures that are registered with and disposed within the plurality of microwells. A method for performing an assay includes flowing cells and a first aqueous medium into a plurality of microwells of a microfluidic device, wherein each microwell includes a cell trap structure configured to trap at least one cell. The method further comprises flowing a nonpolar fluid with low permeability for analytes of interest through a microfluidic channel to flush a portion of the first aqueous medium from the microfluidic channel while retaining another portion of the first aqueous medium and at least one cell within each microwell. Surface tension at a non-polar/polar medium interface prevents molecule exchange between interior and exterior portions of microwells.

Bodily fluid sample collection systems, devices, and method are provided. The sample is collected at a first location and subjected to a first sample processing step. The sample may be shipped to a second location and subjected to a second sample processing step that does not introduce contaminants into a plasma portion of the sample formed from the first processing step. The sample may also be mixed with other material(s) in the collection device.