Described herein are devices and methods for high throughput purification of particles. In some cases, methods and devices described herein can be used to remove erythrocytes and purify leukocytes and raise the quality of umbilical cord blood and other transplant grafts, thereby significantly improving patient outcomes.

The present invention relates to a container for centrifugation. The container for centrifugation includes: a main body (100) including a first chamber (110) in which a material to be centrifuged is received, a second chamber (120) in which a suspended material centrifuged from the material in the first chamber (110) is decanted from the first chamber (110) and received and which is positioned on one side of the first chamber (110), and a coupling part (130) formed to surround the first chamber (110) and the outside of the upper end of the second chamber (120); and a cover (200) which covers an upper portion of the main body (100) and forms a fluid communication path (P) of the decanted suspended material between the first chamber (110) and the second chamber (120).

In accordance with some embodiments, a fluid sample collection and retrieval apparatus including a microfluidic chip, a retrieval tube, a first switch, a second switch and a processor is provided. The microfluidic chip includes a first sample channel, a first fluid directing channel assembly, a first confluence chamber, a first collection channel, a first waste channel, and a retrieval hole. The retrieval hole passes through an outer surface of the microfluidic chip. The retrieval tube is connected to the retrieval hole. The first switch is connected to the microfluidic chip. The second switch is attached to the retrieval tube. The processor is configured to activate the first switch to operate the flow adjustment of the first fluid directing channel assembly and activate the second switch to operate a sample collection in the first collection channel within duration of operating the flow adjustment of the first fluid directing channel assembly.

This liquid handling apparatus has a single introduction part that opens on a first surface side of a substrate and is for introducing a liquid, a plurality of discharge parts that open on the first surface side of the substrate and are for discharging the liquid that has been introduced through the single introduction part, a flow path for connecting the single introduction part and the plurality of discharge parts within the substrate, and a plurality of outflow prevention parts that are disposed so as to surround the openings of the plurality of discharge parts and are for using the surface tension of the liquid to check the progress of the outflow of the liquid from the discharge parts. For each opening part, there are two or more outflow prevention parts disposed so as to surround the opening part.

The embodiments disclosed herein generally relate to systems, devices and methods for the fractionation, isolation, extraction and processing of the adipose supernatant layer of a bone marrow aspirate. In particular, the various embodiments relate to systems devices and methods of obtaining, utilizing and processing the adipose supernatant layer of a bone marrow aspirate as a source of mesenchymal stem cells.

Microfluidic devices that are configured to use centrifugal forces to bias particles into one or more capture regions based on their individual sizes are described.

A substrate for sample analysis including: a substrate including a rotation axis; a first chamber, which includes a first space which retains the liquid; a second chamber, which includes a second space which retains the liquid discharged from the first chamber; and a first flow passage, which includes a path connecting the first chamber and the second chamber in which the first flow passage has a first opening and a second opening, the first opening and the second opening are connected to the first chamber and the second chamber, respectively, and the first opening is positioned on a side closer to the rotation axis than the second opening, in which the first space includes a first region, which includes a portion extending from the first opening and in which the first space of the first chamber has a capacity larger than a capacity of the first flow passage.

A microfluidic device capable of trapping contents in a manner suitable for high-throughput imaging is described herein. The microfluidic device may include one or more trapping devices, with each trapping device having a plurality of trapping channels. The trapping channels may be configured to receive contents via an inlet channel that connects a sample reservoir to the trapping channels via fluid communication. The trapping channels are shaped such that contents within the trapping channels are positioned for optimal imaging purposes. The trapping channels are also connect to at least one exit channel via fluid communication. The fluid, and contents within the fluid, may be controlled via hydraulic pressure.

A microfluidic thermalization chip, a system using such a chip and a PCR method for detecting DNA sequences. The chip contains a block of material in which a cavity is configured to contain at least one fluid. The cavity includes at least one inlet orifice and at least one outlet orifice. The inlet orifice for the fluid is connected to at least one, preferably at least two, fluid-injecting channels. Further, the chip includes at least one microfluidic channel for bypassing the cavity. The channel is connected by a first end to at least one of the fluid-injecting channels. The junction between the bypassing channel and the fluid-injecting channel is located at a distance L from the inlet orifice of the fluid-injecting channel. The distance L is preferably smaller than 2 cm.

In one illustrative embodiment, a cannulated vial is provided, the cannulated vial comprising a vial body having a top surface at one end, a bottom surface at an opposite end, and exterior wall therebetween defining an interior vial volume, the top surface having an opening, a cannula extending from the bottom surface and having a first end, a second end and an outer surface therebetween defining a cannula volume, the first end in fluid communication with the interior vial volume, and a cap having a tongue, the tongue sized to sealably close the opening, the tongue further having a volume greater than or equal to the cannula volume.