A material for manipulating liquid volumes includes a porous substrate having first and second surfaces; and a liquid-manipulating pattern disposed on the first surface, the pattern having a first reservoir connected to a target point via a first wedge-shaped transport element to enable liquid transport from the target point to the first reservoir regardless of gravity, wherein the first surface is one of hydrophobic or superhydrophobic, and wherein the first wedge-shaped transport element is one of superhydrophilic when the first surface is hydrophobic, superhydrophilic when the first surface is superhydrophobic, and hydrophilic when the first surface is superhydrophobic. The pattern can include a second reservoir connected to the target point via a second wedge-shaped transport element to enable liquid transport from the target point to the second reservoir regardless of gravity.

The present disclosure relates to systems and methods for whole cell analysis. In particular, the present disclosure relates to single cell genomic analysis (e.g., gene expression analysis.

A method of handling a fluidic sample in a sample separation device includes at least partly immobilizing the fluidic sample by an immobilizing agent inhibiting spatial broadening of the fluidic sample, and subsequently at least partly releasing the fluidic sample from the immobilizing agent.

A device for performing immunoassay tests includes a readable device for performing immunoassay tests having a platform with at least two sample receiving units. Each sample receiving unit is fluidly connectable to at least two test units, each of which includes a reagent incubation chamber fluidly connected to a separation unit connected to a detection chamber. The platform includes a volume indication unit for detecting the presence of a predetermined volume of the liquid biological sample, and a reading device for performing immunoassay tests. A recognition unit recognizes the presence of the readable device and/or extracts information from a legible identification unit of the readable device. A first optical sensor detects the presence of a binding pair of molecules in the separation unit and/or the presence of free molecules in the detection chamber. A second optical sensor detects the presence of a liquid biological sample in the overflow chamber.

A filter film includes a through-hole and a recessed portion having a size capable of capturing one particle, in which the recessed portion is open to one face of the filter film, the through-hole in the one face has a shape or a size such that the one particle is not capable of passing through the through-hole, and the through-hole and the recessed portion are disposed close to each other.

Beads with functionalized material applied to them are exposed to an acoustic field to trap, retain or pass the beads. The beads may include or be free of ferro magnetic material. The beads may be biocompatible or biodegradable for a host. The size of the beads may vary over a range, and/or be heterogenous or homogenous. The composition of the beads may include high, neutral or low acoustic contrast material. The chemistry of the functionalized material may be compatible with existing processes. The acoustic field may be generated, for example, in an acoustic angled wave device or in an acoustic fluidized bed.

The invention relates to microfluidic devices and array disks for one-pot isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.

Fluidic devices with a primary transport nanochannel(s) intersected by at least one nanoscale manifold for active control of capture, manipulation and transport of analyte molecules. The at least one manifold can be an array or network of nanochannels, nanoslits or nanoelectrodes joined to a common voltage or pressure source. A respective nanoscale manifold can be configured to allow for precise and active control of driving forces applied to the primary transport nanochannel(s) to drive molecular transport through the various regions along the transport nanochannel(s). The at least one manifold can generate monotonic force gradients with a limited or reduced number of independent input potentials and/or pressures applied to the device.

Under one aspect, a device is provided for use in luminescent imaging. The device can include a photonic superlattice including a first material, the first material having a first refractive index. The first material can include first and second major surfaces and first and second pluralities of features defined through at least one of the first and second major surfaces, the features of the first plurality differing in at least one characteristic from the features of the second plurality. The photonic superlattice can support propagation of a first wavelength and a second wavelength approximately at a first angle out of the photonic superlattice, the first and second wavelengths being separated from one another by a first non-propagating wavelength that does not selectively propagate at the first angle out of the photonic superlattice.

Disclosed herein are microfluidic actuators for selecting objects in a fluid stream comprising a plurality of objects. In some embodiments, the actuator comprises an object detection means adapted for, upon arrival of an object, identifying whether an object is an object of interest. It further comprises a heater adapted for generating a jet flow for deflecting an object of interest from the fluid stream and a controller for activating the heater as function of the detection of an object of interest using a nucleation signal. The controller is adapted for obtaining temperature information of the heater and for adjusting a nucleation signal for the heater taking into account the obtained temperature information. Also disclosed are microfluidic systems and diagnostic devices comprising the microfluidic actuators of the disclosure, as well as methods of use thereof.