The present invention provides a fluidic device, an exosome analysis method, a biomolecule analysis method, and a biomolecule detection method, which can analyze even the content of an exosome in a series of flows by introducing a sample into the device. A fluidic device of the present invention is a fluidic device which detects a biomolecule contained in an exosome in a sample, and includes: an exosome purification unit which has a layer modified with a compound having a hydrophobic chain and a hydrophilic chain; a biomolecule purification unit; a biomolecule detection unit; a first flow path which connects the exosome purification unit to the biomolecule purification unit; and a second flow path which connects the biomolecule purification unit to the biomolecule detection unit.

A multi-index detection microfluidic chip is provided. A bottom plate (1) and a cover plate (2) that matches the bottom plate (1) and seals it are provided in the microfluidic chip. The center of the microfluidic chip has a through-hole (3). The bottom plate (1) has one or more wave-shaped main channel(s) (4), one end of each of the main channel (4) connected to a sample injection hole (9) on the bottom plate (1), and the other end connected to an exhaust hole (10) on the bottom plate (1). The valley on the main channel (1) is distal to the through-hole (3), and the peak is proximal to the through-hole (3). Each valley of the main channel (1) is connected to a reaction chamber (6) by a linking channel (5). The linking channel (5) comprises one or more buffering chambers (7). The microfluidic chip can be used in detection by fluorescence, turbidity, color, detection equipment, and/or direct observation by the naked eyes. The detection is real-time as the reaction occurs or after the reaction.

A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.

A microfluidic bead-packing method includes activating a first micropump to transfer active microbeads through an inlet microchannel from a bead suspension reservoir to an adsorbing channel; packing the microbeads in the adsorbing channel; and activating a second micropump to reverse flow through at least a portion of the inlet microchannel and to transfer a sample fluid through the inlet microchannel from a sample reservoir to the adsorbing channel such that the sample fluid interacts with the packed microbeads.

A system for enriching target cells in a sample is disclosed. The system comprises a microfluidic chip comprising a sample inlet, a sample outlet, a waste outlet and a microfluidic channel, the sample inlet being coupled to an upstream end of the microfluidic channel and the sample outlet and the waste outlet being coupled to a downstream end of the microfluidic channel, the microfluidic chip being configured such that target cells are directed to the sample outlet and non-target cells are directed to the waste outlet; a sample input pump configured to introduce the sample into the sample inlet of the microfluidic chip; an output flow rate detector configured to measure a flow rate at the sample outlet and/or the waste outlet of the microfluidic chip; a sample collection switching valve having a configuration switchable between a first configuration in which the sample output of the microfluidic chip is coupled to a sample output container and a second configuration in which the sample output of the microfluidic chip is coupled to a waste output container; and a controller configured to control the configuration of the sample collection switching valve using the flow rate measured by the output flow rate detector.

A fluidic processor device and a wafer including the same, the device including a nanofluidic separator chip including a nanoDLD array, a housing for housing the chip including a top plate disposed on a topside of the chip, a bottom plate disposed on a backside of the chip and fastened to the top plate, and a spacer disposed between the chip and the bottom plate to create a clearance between the chip and the bottom plate for forming a drain space on the backside of the chip.

The disclosure relates to microfluidic devices and methods of use thereof for monitoring the directionality, velocity, and migration persistence of neutrophils or other cells in the absence of chemical gradients for the purposes of detecting and quantifying abnormal neutrophil motility phenotypes, using low sample volumes and with minimal activation of the neutrophils. The devices and methods can be used to diagnose sepsis in subjects suspected of having sepsis or at risk of developing sepsis. The devices and methods can also be used to monitor the responses of patients to sepsis therapies.

A microfluidic chip system includes an input for receiving the biologic sample, and a first reading window for enabling a detection of the biologic material within the biologic sample. A first plurality of pathways is provided each for determining a treatment agent providing a best treatment efficacy for the predetermined biologic material. A first micro-pump is provided for pumping a portion of the biologic sample into each of the first plurality of pathways. A second plurality of pathways is provided, each for determining a dosage level of a particular one of the plurality of treatment agents with respect to the predetermined biologic material. A plurality of second micro-pumps are provided for pumping a second portion of the biologic sample into a selected one of the second plurality of pathways responsive to the determination of treatment efficacy of the treatment agent providing a best treatment of the predetermined biologic material.

An integrated microfluidic chip, wherein at least one integrated reaction unit is provided on its substrate, and the integrated reaction unit comprises at least a sample cell (1), a mixing cell (2) and a reaction cell (3) connected through liquid channels (6). In one aspect, one end of the sample cell (1) is provided with a sample inlet (4), and the chip further comprises an internal air circulating system/circuit. One end of the internal air circulating system/circuit is connected with the mixing cell (2), while the other end comprises at least a first circulation branch circuit connected with the end of the sample cell (1) distal to the sample inlet (4).

A particle manipulation system uses a MEMS-based, microfabricated particle manipulation device which may be used to separate a target particle from non-target material in a sample stream. In order to improve the sorter speed, accuracy or yield, the particle manipulation system may also include a microfluidic structure which focuses the target particles in a particular portion of the sample inlet channel. The particle manipulation device may have two separate sort output channels, wherein the sort channel used depends on the characteristics of the sort pulse delivered to the micromechanical particle manipulation device. Because of the improved focusing and pulse details, a droplet may be formed which contains a single particle, which may also be barcoded with an identifiable signature bead.