Disclosed herein are example embodiments of a transformative sensor apparatus that is capable of detecting and quantifying the presence of a substance of interest such as a specified bacteria within a sample via changes in impedance exhibited by a detection electrode array. In an example embodiment, sensitivity is improved by including a focusing electrode array in a rampdown channel to focus a concentration of the substance of interest into a detection region. The focusing electrodes include an opposing pair of electrodes in a rampdown orientation. The focusing electrode may also include tilted thin film finger electrodes extending from the rampdown electrodes. In another example embodiment, trapping electrodes are positioned to trap a concentration of the substance of interest onto the detection electrode array.

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

A system for determining drug effectiveness on a plurality of cells is described. The system includes flowing a ferrofluid mixed with a plurality of biological cells through an inlet portion of a cartridge, the cartridge comprising a plurality of microfluidic channels, the inlet is in communication with a portion of each of the plurality of channels, applying a magnetic field proximate at least one of the inlet portion and the plurality of micro-channels, wherein the magnetic field is configured to apply an indirect force on the mix, separating biologic cells according to at least a first type as the mix flows in a first direction; and directing at least the first type of cells toward a first sensor functionalized with receptors via at least one of the micro-channels, the sensor arranged proximate to a second portion of at least one of the micro-channels downstream from the first inlet portion.

The present invention generally relates to microfluidic devices. In some aspects, various entities, such as droplets or particles, may be contained within a microfluidic device, e.g., within collection chambers or other locations within the device. In some cases, the entities may be released from such locations, e.g., in a sequential pattern, or an arbitrary pattern. In some cases, the entities may be imaged, reacted, analyzed, etc. while contained within the collection chambers. Other aspects are generally directed to methods of making or using such devices, kits involving such devices, or the like.

An interface is provided for storing microfluidic samples in a nanoliter sample chip. A fluid access structure provides a fluid access region to a selected subset of sample wells from an array of sample wells. A fluid introduction mechanism introduces a sample fluid to the fluid access region so that the sample wells in the selected subset are populated with the sample fluid without the unselected sample wells being populated with the sample fluid.

A microfluidic device (10) for full blood count includes a first measurement channel (11) and a second measurement channel (12) separated from the first measurement channel (11). The microfluidic device (10) furthermore includes a first inlet (13) for providing a whole blood sample to the first and second measurement channel (11, 12), a second inlet (14) for providing a lysis agent for white blood cell count in to the first channel (11), a third inlet (15) for providing a quench solution to the first channel (11), and a fourth inlet (16) for providing a lysis agent for hemoglobin measurement to the second channel (12). A method for forming such a microfluidic device (10) and a method for performing a full blood count test using such a microfluidic device (10) are described.

The invention relates to a device for hydrating with a liquid sample, the device comprising a container (4) for receiving at least one liquid sample, and a lid (5) possessing a bottom face (5.sub.1) having at least one hydrating support (3) fastened thereto to present an absorption face (3.sub.1) for absorbing a liquid sample. According to the invention, the container (4) presents, by means of at least one well (6), a calibrated volume for receiving a liquid sample, the at least said well presenting a hydrating calibrated open section for hydrating a hydrating support (3) defined by the top edge (8) of at least said well (6), the hydrating calibrated open section possessing an area that is less than the area of the absorption face of the hydrating support (3) in order to control the absorption by capillarity of the liquid sample by the hydrating support.

A nucleic acid amplification disk apparatus using a temperature sensitive polymer synthesis and the analysis method using the same, and more specifically, and the nucleic acid amplification device, and the analysis method using the nucleic acid amplification disk unit and the nucleic acid amplification disk for amplifying the Bacterial DNA or RNA, and the driving control section for controlling the nucleic acid amplification disk.

Aspects of the present disclosure include branching fluid passages in an apparatus that reduce turbulent flow and generate evenly distributed fluid pressure as the fluids branch off into the different passages. In some embodiments, the branching passages may be subdivided into two sets: the branching passages for the liquid fuel and the branching passages for the liquid oxidizer. In some embodiments, the two sets of passages are carefully designed in an elegant yet extremely intricate manner that is optimized for proper fluid flow and maximal burn efficiency. The ends of all of the passages meet at the injector interface, which dispense the liquids into the combustion chamber for ignition. Generally, these designs are achieved through additive manufacturing, and would be extremely difficult, if not impossible, to be manufactured using traditional techniques.

A microfluidic device for detecting a target gene according to the present invention comprises a plurality of capillary tubes which are partially immersed in a sample container containing sample solution and in which the sample solution flows by capillary phenomenon, and microbead packings arranged at one part in each capillary tube to be arranged on a flow path of the sample solution, wherein each of the microbead packings comprises: a packing tube arranged at the capillary tube so as to partially constitute the flow path of the sample solution, a plurality of microbeads contained in the packing tube and being in close contact with each other to form voids between the microbeads, and probe linkers formed on a surface of each microbead, wherein the probe linkers are configured to amplify a target gene in the sample solution by complementary bonding with the target gene, thereby detecting the target gene.