The present disclosure relates to an apparatus, method, and system for separating a fluid sample. The apparatus for separating a fluid sample may include a receiving chamber comprising a first opening and a second opening. The first opening may have a larger diameter than the second opening. The apparatus may include a channel extending from the second opening of the receiving chamber. The channel may include a proximal end adjacent the receiving chamber and a distal end. The distal end of the channel may bifurcate into a first branch and a second branch. The first branch and the second branch can be inserted into a first container and a second container through a cap having an orifice to separate a fluid sample into the first container and the second container.

A method for fabricating an in vitro vessel includes forming a substrate that defines a microfluidic passage therein extending along a longitudinal axis and defined by an inner surface, positioning the substrate in a vertical orientation whereby an acute angle is formed between the longitudinal axis of the microfluidic passage and the direction of gravity, and culturing a plurality of first cells in the microfluidic passage while the substrate is disposed in the vertical orientation whereby an annular layer of the plurality of first cells is formed in the microfluidic channel, wherein the layer of the plurality of first cells defines a lumen extending longitudinally through the microfluidic channel.

The present invention is directed to sample test cards having an increased sample well capacity for analyzing biological or other test samples. In one embodiment, the sample test cards of the present invention comprise one or more fluid over-flow reservoirs, wherein the over-flow reservoirs are operatively connected to a distribution channel by a fluid over-flow channel. In another embodiment, the sample test cards may comprise a plurality of flow reservoirs operable to trap air thereby reducing and/or preventing well-to-well contamination. The test card of this invention may comprise from 80 to 140 individual sample wells, for example, in a test card sample test cards of the present invention have a generally rectangular shape sample test card having dimensions of from about 90 to about 95 mm in width, from about 55 to about 60 mm in height and from about 4 to about 5 mm in thickness.

Microfluidic methods for barcoding nucleic acid target molecules to be analyzed, e.g., via nucleic acid sequencing techniques, are provided. Also provided are microfluidic, droplet-based methods of preparing nucleic acid barcodes for use in various barcoding applications. The methods described herein facilitate high-throughput sequencing of nucleic acid target molecules as well as single cell and single virus genomic, transcriptomic, and/or proteomic analysis/profiling. Systems and devices for practicing the subject methods are also provided.

A multi-channel microfluidic blood coagulation detection chip having a five-layer structure includes a chip body. The chip body includes, in sequence from top to bottom, a first-layer chip, a second-layer chip, a third-layer chip, a fourth-layer chip, and a fifth-layer chip. The first-layer chip (1), the second-layer chip, the third-layer chip, the fourth-layer chip, and the fifth-layer chip cooperate with each other to define a closed microfluidic channel and a plurality of mutually-independent detection chambers. The first-layer chip is provided with a sample loading hole, and the sample loading hole communicates with the detection chambers through the microfluidic channel. The chip body further includes electrodes, and the electrodes are disposed within the detection chambers in one-to-one correspondence.

1. A method of performing an acoustophoretic operation comprises the steps of: i. providing a fluid, ii. positioning the fluid in a microfluidic cavity, iii. subjecting at least one portion of the fluid, in the microfluidic cavity, to an acoustic wave, and iv. providing, in at least one first region of the at least one portion, a thermal inhomogeneity whereby the temperature of the fluid in the at least one first region differs from the temperature of the fluid in at least one second region of the remainder of the at least one portion. A microfluidic system is also disclosed.

A multi-channel microfluidic blood coagulation detection chip includes a chip body. The chip body includes a lower-layer chip, a middle-layer chip, and an upper-layer chip in sequence from bottom to top. The lower-layer chip, the middle-layer chip, and the upper-layer chip cooperate with each other to define a closed microfluidic channel and a plurality of mutually-independent detection chambers. The upper-layer chip is provided with a sample loading hole, and the sample loading hole communicates with the detection chambers through the microfluidic channel. The chip body further includes electrodes. The electrodes include upper-layer electrodes and lower-layer electrodes, the upper-layer electrodes are disposed on a back surface of the upper-layer chip, the lower-layer electrodes are disposed on a front surface of the lower-layer chip, and a gap is provided between the upper-layer electrodes and the lower-layer electrodes.

In example implementations, a cell trapping array is provided. The cell trapping array includes a plurality of plates coupled along adjacent edges to form a channel. A plurality of orifices are formed in a first plate of the plurality of plates of the channel. The plurality of orifices is shaped to create a meniscus of a fluid in the channel in the plurality of orifices that is to attract a single cell from cells flowing through the channel in the fluid. The cell trapping array includes a selective ejection system coupled to a second plate located opposite the first plate of the channel. The selective ejection system is to selectively eject the single cell from one of the plurality of orifices.

A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.

A device and method is described to parallelize a pressure-volume-temperature (“PVT”) analysis using gas chromatography and mass spectrometry techniques such that a portion of the pressure, temperature and volume analysis is performed separately from others. The resulting PVT data is then recombined statistically for a complete PVT analysis. The device may also obtain compositional data of the fluid to perform an equation of state analysis or reservoir simulations.