The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalising two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; wherein one or both of steps (a) and (b) is performed under microfluidic control; preferably electronic microfluidic control The invention further allows for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, and which is co-compartmentalised into the microcapsules.

A microfluidic device comprising one or more fluidic microchannels and one or more arrays of cell assay units is disclosed. Each cell assay unit in turn comprises at one bipolar electrode, micropocket, reaction chamber, and leak channel. In some embodiments, the cell assay unit further comprises two or more split BPEs inside the reaction chamber. The disclosed microfluidic device can be used to separate cells, especially rare cells, from its biological matrix and then analyze the isolated cell inside the reaction chamber. The disclosed device can isolate and analyze cells in a high-throughput fashion and without any modification or labelling to the cells. Cells isolated using the disclosed devices does not lose their vitality.

A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.

Methods, devices, and systems for performing isoelectric focusing reactions are described. The systems or devices disclosed herein may comprise fixtures that have a membrane. In some instances, the disclosed devices may be designed to perform isoelectric focusing or other separation reactions followed by further characterization of the separated analytes using mass spectrometry. Two or more isoelectric focusing reactions may be performed in parallel. The disclosed methods, devices, and systems provide for fast, accurate separation and characterization of protein analyte mixtures or other biological molecules by isoelectric point.

A microfluidic passive sample separation device for passively separating a liquid sample, and optionally detecting an analyte in the separated liquid sample. The device includes a filter for filtering the liquid sample and for producing a filtrate; a plurality of capillaries configured to withdraw the filtrate from the filter by capillary force, a capillary micropump for receiving the filtrate and pumping the filtrate to the outlet.

A mixer includes a mixing chamber and a motor mechanically connected to the mixing chamber. The mixing chamber comprises a suspended elongate rigid tube section having a top end and an open bottom end, and a flexible tube section extending downwards from the open bottom end. The motor comprises a vibration motor mechanically coupled to the suspended elongate rigid tube section at the top end of the suspended elongate rigid tube section.

The disclosure relates to a microfluidic control chip. The microfluidic control chip may include an upper cover, a lower cover, and a chip functional layer between the upper cover and the lower cover. The chip functional layer may include a first region. The chip functional layer in the first region may include at least one chamber unit, an inlet flow channel to the chamber unit, and an outlet flow channel from the chamber unit. The chamber unit may include a main flow channel, a plurality of secondary flow channels, and a plurality of microcavity structures. The chamber unit may be configured to allow a liquid to flow from the main flow channel to the plurality of secondary flow channels, and then to the plurality of microcavity structures.

This present disclosure provides devices, systems, and methods for performing point-of-care analysis of a target analyte in a biological fluid via a binding assay. The present disclosure includes a cartridge for collecting the target analyte contained in a fluid sample and performing an assay. The cartridge includes an assay stack having a first separation layer, a second separation layer, and a detection membrane. The cartridge also includes a plurality of first complexes comprising a capture molecule and a magnetic bead and a plurality of second complexes comprising a detection molecule and a detection label. Further, the detection membrane includes a substrate that interacts with the detection label to elicit a quantifiable response in the presence of the target analyte. The quantifiable response corresponds to an amount of detection antibody present in the detection membrane, and the amount of detection antibody present corresponds to an amount of the target analyte present.

A sensor for detecting a target pathogen (e.g., a virus or a bacterium) in a specimen is disclosed, which includes at least two sensing units one of which is configured to detect at least one protein (such as a structural protein) associated with the target pathogen and another one is configured to detect at least one genetic component (e.g., an RNA or a DNA segment) associated with that pathogen (e.g., an RNA segment that is unique to that pathogen).

An embodiment includes a sample receiving region, a first diagnostic element that includes one or more colorimetric analysis regions, and a second diagnostic element that includes one or more lateral flow assay analysis regions. The embodiment also includes a first flow path that allows a portion of a liquid deposited at the sample receiving region to flow to the first diagnostic element. The embodiment also includes a second flow path that allows a portion of the liquid deposited at the sample receiving region to flow to the second diagnostic element.