The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.

Systems, methods, and devices for discretizing and analyzing fluidic samples are provided. In one aspect, a microfluidic array for discretizing a fluidic sample comprises one or more flow channels and a plurality of fluidic compartments in fluidic communication with the one or more flow channels. In another aspect, a system for discretizing and analyzing fluidic samples comprises a rotor assembly shaped to receive a microfluidic device.

A fluid line part for a microfluidic device includes a first substrate having a first surface in which at least one depression is provided, the depression forming a channel for conducting a fluid along a main flow direction. At least one support web extends lengthwise inside the channel along the main flow direction. The support web is configured and positioned such that fluid flows freely around it.

A single junction sorter for a microfluidic particle sorter, the single-junction sorter comprising: an input channel, configured to receive a fluid containing particles; an output sort channel and an output waste channel, each connected to the input channel for receiving the fluid therefrom; a bubble generator, operable to selectively displace the fluid around a particle to be sorted and thereby to create a transient flow of the fluid in the input channel; and a vortex element, configured to cause a vortex in the transient flow in order to direct the particle to be sorted into the output sort channel.

A digital dispense apparatus includes at least one fluid dispense device, at least one reservoir fluidically connected to the at least one fluid dispense device, a monolithic carrier structure carrying the at least one fluid dispense device and reservoir, the monolithic carrier forming fluid routing between the reservoir and the fluid dispense device.

Disclosed herein are embodiments of fluidic devices that can be used to detect the presence (or absence) of analytes in samples by providing separate and distinct chromatographic signals for particular analytes. The fluidic devices described herein are highly sensitive and user-friendly. Methods of making and using the disclosed fluidic devices also are disclosed herein.

A microfluidic chip for partitioning a liquid composition into multiple aliquots has a processing compartment including an inlet and an outlet, wherein the outlet of the processing compartment is connected either to an inlet of the compression compartment or wherein in case a compression channel is present, the outlet of the processing compartment is connected to the compression channel, which is connected to the inlet of the compression compartment, preferably wherein each of the compression compartments exhibits the same volume.

The present invention discloses a droplet digital PCR chip. The droplet digital PCR chip includes at least one chip unit, each chip unit includes a chip body formed by bonding a top piece and a bottom piece, the chip body is internally provided with an inlet chamber, a droplet storage chamber, and an injection hole. The injection hole connects with the inlet chamber, a plurality of droplet generating channels are disposed between the inlet chamber and the droplet storage chamber, a height of the droplet generating channel is smaller than a height of the droplet storage chamber, an injection fluid is injected into the inlet chamber through the injection hole, and the injection fluid is emulsified and enters the droplet storage chamber at a junction of the droplet generating channels and the droplet storage chamber.

Methods and apparatus for manufacturing and operating a microfluidic arrangement are disclosed. In one arrangement, a continuous body of a first liquid is provided in direct contact with a first substrate. A second liquid is provided in direct contact with the continuous body of first liquid and covering the continuous body of first liquid, the second liquid being immiscible with the first liquid. A separation fluid, immiscible with the first liquid, is propelled through at least the first liquid and into contact with the first substrate over all of a selected region on the surface of the first substrate, thereby displacing first liquid that was initially in contact with the selected region away from the selected region without any solid member contacting the selected region directly and without any solid member contacting the selected region via a globule of liquid held at a tip of the solid member, the selected region being such that one or more walls of second liquid are formed that modify a shape of the continuous body of first liquid.

A plasmon resonance system, instrument, cartridge, and methods for analysis of analytes is disclosed. A PR system is provided that may include a DMF-LSPR cartridge that may support both digital microfluidic (DMF) capability and localized surface plasmon resonance (LSPR) capability for analysis of analytes. In some examples, the DMF portion of the DMF-LSPR cartridge may include an electrode arrangement for performing droplet operations, whereas the LSPR portion of the DMF-LSPR cartridge may include an LSPR sensor. In other examples, the LSPR portion of the DMF-LSPR cartridge may include an in-line reference channel, wherein the in-line reference channel may be a fluid channel including at least one functionalized LSPR sensor (or sample spot) and at least one non-functionalized LSPR sensor (or reference spot). Additionally, methods of using the PR system for analysis of analytes are provided.