A test apparatus for a test using a test solution containing a sample with the use of a microchannel device is provided. The test apparatus includes a pressure application unit that is connected to a first opening and applies an air pressure to inject the test solution into a microchannel, an opening and closing unit that switches between opening and closing of a second opening, and a controller that controls the pressure application unit and the opening and closing unit. The controller controls the opening and closing unit to close the second opening, controls the pressure application unit to inject the test solution into the microchannel, controls the opening and closing unit to open the second opening after the test solution is injected into the microchannel, and controls the pressure application unit to apply an air pressure to collect in a collection portion, the test solution in a branch channel.

A point of care cassette, comprising: a housing; a lancet carrier for carrying a lancet, the lancet carrier being configured to be retractably urged by a spring from a locked position to a deployed position in response to an actuation of a first trigger, the lancet being entirely within the housing when not in the deployed position; and a test carrier for carrying a testing medium, the test carrier configured to be urged by a second spring from a locked position to a deployed position in response to an actuation of a second trigger, the second trigger being actuated during deployment of the lancet carrier to bring the testing medium proximate a blood sample after retraction of the lancet.

A portable, closed cell incubating cassette system and method including: an inlet to receive a volume of a liquid sample and fluidly connected to one or more incubating channels, each of which including one or more gates located in the one or more incubating channels, each of the gates having an open position and a closed position, wherein each of the gates when in the open position is configured to permit the sample to fluidly connect to a remaining portion of each of the incubating channels, and wherein each of the gates when in the closed position is configured to block the sample from fluidly connecting to the remaining portion of each of the incubating channels; and one or more sample incubation chambers each disposed in each of the incubating channels between the inlet and each of the gates.

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

A fluidic device (100) is described for locally coating an inner surface of a fluidic channel. The fluidic device (100) comprises a first (101), a second (102) and a third (103) fluidic channel intersecting at a common junction (105). The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device (100) further comprises a fluid control means (111) configured for creating a fluidic flow path for a coating fluid at the common junction (105) such that, when coating, a coating fluid propagates from the first (101) to the second (102) fluidic channel via the common junction (105) without propagating into the third (103) fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

An apparatus for separation of cells in a liquid into subsets of cells comprises a microfluidic chip comprising: a microfluidic channel having a liquid inlet for receipt of a stream of cell containing fluid; a detection zone disposed in the microfluidic channel and comprising a sensor configured to detect changes in the microfluidic channel corresponding to cells passing the sensor; and a separation zone distal of the detection zone in which the microfluidic channel divides into at least two secondary microfluidic channels, the separation zone comprising two or more separation electrodes, wherein the two or more separation electrodes include at least one separation electrode disposed in electrical contact with an interior of the microfluidic channel and at least one further separation electrode disposed in electrical contact with an interior of the microfluidic channel or one of the secondary microfluidic channels.

This disclosure relates to an apparatus for simultaneously filling a plurality of sample chambers. In one aspect, the apparatus comprises a common fluid source and a plurality of independent, continuous fluidic pathways. Each independent, continuous fluidic pathway comprises a sample chamber and a pneumatic compartment. The sample chamber is connected to the common fluid source, and the pneumatic compartment is connected to the sample chamber. The sample chamber comprises, in part, an assay chamber. The assay chamber comprises a monolithic substrate and a plug having optically transmissive properties. In some embodiments, the assay chamber contains a magnetic mixing element. In some embodiments, the assay chamber is a double tapered chamber. In some embodiments, a ratio of a volume of the sample chamber to a volume of the pneumatic compartment is substantially equivalent for each fluidic pathway of the plurality of fluidic pathways.

A biosensor apparatus is provided. The biosensor apparatus includes a base substrate; a first fluid channel layer on the base substrate and having a first fluid channel passing therethrough; a foundation layer on a side of the first fluid channel layer away from the base substrate, a foundation layer throughhole extending through the foundation layer to connect to the first fluid channel; and a micropore layer on a side of the foundation layer away from the base substrate, a micropore extending through the micropore layer to connect to the first fluid channel through the foundation layer throughhole. The micropore layer extends into the foundation layer throughhole and at least partially covers an inner wall of the foundation layer throughhole.

Provided is a technology for preparing a mixture including multiple types of microparticles in accordance with a predetermined ratio. The present technology provides a microparticle sorting device (100) including a control unit (103) comprising a determination unit that determines whether microparticles are sorted, on the basis of light generated by irradiating, with light, the microparticles flowing through a flow channel (155), in which the determination unit performs a primary sorting determination to determine, on the basis of characteristics of the light generated, whether the microparticles belong to any one of two or more different microparticle populations, and then performs a secondary sorting determination to determine whether the microparticles determined to belong to any one of the microparticle populations in the primary sorting determination are sorted, on the basis of the particle constituent ratio specified for the two or more different microparticle populations. This technology may be used to prepare a cell therapeutic agent comprising a mixture of cells of different types in a predefined ratio, e.g. a mixture of various types of CAR T-cells showing a synergistic anti-tumor activity.

Embodiments of the invention relate to centrifugal fluidic devices, apparatus, and methods. Embodiments disclosed are fluidic devices, and associated apparatus and methods, which can partition a fluid sample from a single inlet or plurality of inlets into a plurality of chambers via their fluid inlets. Each chamber possesses a fluid outlet and a gas outlet. Partitioned fluid can be further distributed under centrifugal pressure to downstream fluidics modules, permitting various multiplexed assays to be performed including nucleic acid amplification tests.