The present disclosure relates to a consumable sample partition device and it assembly and use. The sample partition device can be used to test a sample for absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). The sample partition device partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.

The present invention provides a detection device comprises a testing element and a transparent area, wherein the testing element comprises a detection area which is configured to detect a presence of an analyte in a liquid sample; the transparent area is configured to read the test result on the detection area through the transparent area; a part of the transparent area contacts a part of the detection area, or the detection area and the transparent area are arranged in one sealed space, thus to make the air in the sealed space not exchange with the air outside the sealed space; the scheme can reduce the mist to ensure the test result is displayed clearly.

A solid reagent containment unit is formed by a support; a frame body fixed to the support and delimiting internally, together with the support, an analysis volume; a reagent-adhesion structure within the analysis volume; and at least one reagent cavity, which extends within the reagent-adhesion structure. The reagent-adhesion structure is of an adhesion material embossable at temperatures lower by 6-8° C. than its own melting point and has a melting point such as not to interfere with the analysis. The reagent cavity forms a retention wall, laterally surrounding the reagent cavity, and houses dried reagents. The adhesion material is chosen among wax, such as paraffin, a polymer, such as polycaprolactone, a solid fat, such as cocoa butter, and a gel, such as hydrogel or organogel.

The present invention relates to a liquid handling and processing tool for analyzing a biological sample. Furthermore, the present invention relates to a fluid processing and detection module for processing a liquid sample and for detecting analyte in the sample.

A technique for detection of probes in a microfluidic flow-through chamber involves a plurality of interface pinning reaction vessel formed by micro- or nano-structured relief patterning of a substrate. The relief patterning increases a surface area locally, and defines a plurality of separated interface pinning reaction vessels. The marked detection protocol may be supplied on a single layer of a stacked microfluidic chip, or the chamber may constitute a whole layer. The chip may be designed to be driven mechanically, pneumatically, hydraulically, centrifugally or by capillary action. Each vessel allows for a high density of probes, an effective region for developer-type or fluorescence-based marking, and efficient readout. Suitable probe liquids can be self-limiting to fill one vessel. Suitable developer liquids avoid dye bleeding across vessels during washing.

There is provided a method of making a fluidic system that comprises assembling a fluidic system comprising a first plate, a second plate and a membrane disposed between the first plate and the second plate; applying laser energy to the fluidic system to cause the first plate, the second plate and the membrane to melt at bonding areas; and allowing the bonding areas to cool down such that the first plate, the second plate and the membrane are bonded together.

The invention relates to a substrate for testing samples, in particular cells or molecules, wherein the substrate comprises a fluid system comprising a sample chamber configured in the substrate for storing and testing samples and at least one liquid reservoir in fluid communication with the sample chamber, and wherein the substrate comprises a passive blocking element capable of assuming a closed position and an open position, wherein in the closed position a fluid exchange between the sample chamber and the liquid reservoir is blocked.

The invention provides devices that improve tests for detecting specific cellular, viral, and molecular targets in clinical, industrial, or environmental samples. The invention permits efficient detection of individual microscopic targets at low magnification for highly sensitive testing. The invention does not require washing steps and thus allows sensitive and specific detection while simplifying manual operation and lowering costs and complexity in automated operation. In short, the invention provides devices that can deliver rapid, accurate, and quantitative, easy-to-use, and cost-effective tests.

In an embodiment, the present disclosure pertains to a microfluidic platform composed of a droplet generator having an entry point for donor particles and target particles, a first droplet incubation chamber in fluid communication with the droplet generator, a droplet detection functionality to allow for analysis of the inner content of droplets, and a droplet sorting functionality to allow for the separation of droplets based on the analysis of the inner content of droplets. In another embodiment, the present disclosure pertains to a method for cell-to-cell DNA, RNA, or other genetic material transfer through use of a water-in-oil emulsion microdroplet-based microfluidic platform for automation and high throughput identification or screening of genetic transfer outcomes utilizing the microfluidic platforms as disclosed herein.

An analyzing system is provided. The analyzing system includes a fluid container defining a sample chamber where a sample is contained in the sample chamber, and a sensor including a transparent body with a reverse face and an obverse face where the obverse face having a nanostructured surface. The nanostructured surface includes a plurality of elongate nanostructures having a respective longitudinal axis that is disposed substantially perpendicularly to the obverse face. The analyzing system includes an excitation and detection apparatus that includes an excitation source for generating a beam of polarized radiation and a corresponding radiation detector where the sensor is coupled to the fluid container such that the nanostructured surface is exposed to the sample chamber, to the sample located therein.