A system for testing a treatment agent for a biologic material includes an input for receiving a biologic sample. A plurality of micro-pumps pump a portion of the biologic sample from the first reservoir into a connected module. A first module includes a first plurality of testing pathways for testing a first portion of the biologic sample. A first module connector removeably connects the first module to the distributor module. A second module includes a second plurality of testing pathways for testing a second portion of the biologic sample. The selected pathway applies at least one dosage level of a treatment agent to the second portion of the biologic sample. A second module connector removeably connects the second module to the distributor module, wherein treatment agent and the plurality of dosage levels tested by the system may be selected by selecting the second module associated with the second module connector.

This disclosure relates to systems and methods for isolating, detecting, and/or analyzing brain-derived cells or particles in the blood circulation of human and animal subjects.

A microfluidic chip system includes an input for receiving the biologic sample, and a first reading window for enabling a detection of the biologic material within the biologic sample. A first plurality of pathways is provided each for determining a treatment agent providing a best treatment efficacy for the predetermined biologic material. A first micro-pump is provided for pumping a portion of the biologic sample into each of the first plurality of pathways. A second plurality of pathways is provided, each for determining a dosage level of a particular one of the plurality of treatment agents with respect to the predetermined biologic material. A plurality of second micro-pumps are provided for pumping a second portion of the biologic sample into a selected one of the second plurality of pathways responsive to the determination of treatment efficacy of the treatment agent providing a best treatment of the predetermined biologic material.

The invention relates to a method for achieving microfluidic perfusion of a spheroid, said method being implemented in a microfluidic device that comprises a cavity (151) for hydrodynamically trapping said spheroid, said method comprising the following steps: performing a first injection of a gel (20) containing said spheroid into the microfluidic network (10), hydrodynamically trapping said spheroid in the trapping cavity (151) of the microfluidic network, performing a second injection of a fluid that is non-miscible with said gel into said microfluidic network with a view to flushing away gel present in the network, except in the trapping cavity (151), cross-linking the gel (20) present around the spheroid, in the trapping cavity (151), performing a third injection of a culture medium (22) into said microfluidic network with a view to perfusing the spheroid petrified in its gelled environment, and located in the trapping cavity (151).

The present disclosure provides a sample test card and a sample loading method thereof, relates to the technical field of microbiological testing. The present disclosure includes sample wells arranged in an array; the sample wells are connected together through a flow channel network, and the sample wells are filled with a sample through a unified intake port. Sample filling is completed by vacuuming; during filling, a liquid sample is firstly filled, and air or other inert gas or insoluble liquid is filled; liquid sample volume and air volume are formed in proportion in the sample wells. In the present disclosure, the filled liquid sample is controlled not to fill the sample wells completely, and there are sufficient air space in sample wells, so that the sample wells are independent from each other to avoid contamination, and more sample wells can be arranged on the test card with the same size.

A biocompatible micropillar array substrate (MAS) and methods for preparing the biocompatible MAS are provided. In on example, the biocompatible MAS includes multiple micropillars made from a biocompatible polymer. The biocompatible MAS may be prepared using a replica fabricated based on a silicon MAS. The configuration of the multiple micropillars of the silicon MAS and a configuration of the multiple micropillars of the biocompatible MAS are the same.

Embodiments of the invention are directed to a method of separating and encapsulating an analyte on a microfluidic device in order to extract the analyte. A microfluidic device is provided having a main microchannel and a set of one or more auxiliary microchannels, each branching to the main microchannel at respective junctions therewith. A mixture is introduced as a single phase in the main microchannel in order to electrokinetically separate an analyte from the introduced mixture, and in order to confine the separated analyte in a microchannel portion of the main microchannel. The microchannel portion adjoins one of the junctions. One or more encapsulating volumes of an encapsulating phase are injected in the main microchannel via one or more of the auxiliary microchannels. The encapsulating phase is immiscible with said single phase. The encapsulated analyte is extracted from the main microchannel via one or more of the auxiliary microchannels.

A microfluidic device comprising a channel within a substrate and a condenser or a hydrodynamic focusing chamber along the channel, configured to focus a fluid containing particles of a plurality of sizes. A negative angle deterministic lateral displacement (DLD) array is configured to receive the focused fluid and separate the particles in the focused fluid into three sizes ranges. The negative angle DLD array comprises a plurality of rows of pillars, wherein the rows of pillars are positioned to repeat a pattern every N rows with a shift of M columns, N and M are relatively coprime, and N is greater than 1.

A microfluidic AM-EWOD device and a method of filling such a device are provided. The device comprises a chamber having one or more inlet ports. The device is configured, when the chamber contains a metered volume of a filler fluid that partially fills the chamber, preferentially maintain the metered volume of the filler fluid in a part of the chamber. The device is configured to allow displacement of some of the filler fluid from the part of the chamber when a volume of an assay fluid introduced into one of the one or more inlet ports enters the part of the chamber, thereby causing a volume of a venting fluid to vent from the chamber.

A system and method for determining a trajectory parameter of particles, comprising receiving a plurality of particles at a microfluidic channel, applying a force to each particle of the microfluidic channel, acquiring a dataset of each particle, measuring a trajectory of the particle, and determining a trajectory parameter of the particles.