One example of a flow cell includes a base support and a multi-layer stack positioned over the base support. The multi-layer stack includes a resin layer positioned over the base support; and a hydrophobic layer positioned over the resin layer. A depression is defined in the multi-layer stack through the hydrophobic material and through a portion of the resin.

Provided is a liquid feeding device, including: a test container including a first accommodation portion, a second accommodation portion, and a third accommodation portion each accommodating a liquid, a first flow path connecting the first accommodation portion and the second accommodation portion to each other at respective upper end positions thereof, and a second flow path connecting the second accommodation portion and the third accommodation portion to each other at respective upper end positions thereof, in which at least a portion forming an upper wall surface of the second accommodation portion has flexibility; and a pressing machine which includes a first pressing portion and presses the portion forming the upper wall surface of the second accommodation portion at a position closer to the first flow path from a center in a liquid feeding direction towards an inside of the second accommodation portion from an outside by the pressing portion, in which the liquid accommodated in the second accommodation portion is fed to the third accommodation portion by pressing the portion forming the upper wall surface of the second accommodation portion with the pressing machine.

A method for continuously separating components from a sample includes providing a field-flow fractionation device including: a channel coupled to a flow generator for translocating the sample components along the channel in a first direction, an actuator for translocating the sample components in a second direction, at an angle with the first direction, and an array of electrodes electrically or capacitively connected to an AC power source, operating the actuator so as to translocate the sample components in a second direction at an angle with the first direction, operating the AC power source so as to generate an AC electric field between adjacent rows, and operating the flow generator, collecting sample components from the sample outlets.

A microfluidic AM-EWOD device and a method of filling such a device are provided. The device comprises a chamber having one or more inlet ports. The device is configured, when the chamber contains a metered volume of a filler fluid that partially fills the chamber, preferentially maintain the metered volume of the filler fluid in a part of the chamber. The device is configured to allow displacement of some of the filler fluid from the part of the chamber when a volume of an assay fluid introduced into one of the one or more inlet ports enters the part of the chamber, thereby causing a volume of a venting fluid to vent from the chamber.

Methods of forming a chip with fluidic channels include forming (e.g., milling) at least one nanofunnel with a wide end and a narrow end into a planar substrate, the nanofunnel having a length, with width and depth dimensions that both vary over its length and forming (e.g., milling) at least one nanochannel into the planar substrate at an interface adjacent the narrow end of the nanofunnel.

The disclosed embodiments generally relate to a method and system to synthesize a target molecule within a droplet. In an exemplary embodiment, a first microfluidic device configured to contact a polynucleotide-containing component from a sample with lysis reagents to form a first droplet. The lysis reagents include an enzyme having protease activity. The first droplet is encapsulated with an immiscible carrier fluid. A collection reservoir is provided to receive and incubate the first droplet for a first duration of time. The first duration of time is sufficient to inactivate the enzyme of the lysis reagent. A second microfluidic device is provided to receive the first droplet and add nucleic acid synthesis reagent to thereby form a second nucleic acid synthesis droplet in the immiscible carrier fluid. Finally, a reaction chamber is provided to synthesize the target polynucleotide within the second nucleic acid synthesis droplet.

A method is provided for manipulating objects in a cavity including a liquid, the method including providing in at least one region of the cavity objects capable of absorbing light in a given wavelength range, forming an aggregate of the objects by submitting them to an acoustic field, and disrupting the aggregate by submitting the aggregate to a light beam emitting at the given wavelength range. Also provided is a device for manipulating objects.

Provided herein are a system and method for using a microfluidics device. The system includes: a plurality of pumps and a plurality of sensors; a first communication line to select a pump from the plurality of pumps and select a sensor from the plurality of sensors; a second communication line selectively connected to the selected pump; and a third communication line selectively connected to the selected sensor.

Disclosed is a detection apparatus that transfers magnetic particles through a plurality of chambers in a cartridge which includes the plurality of chambers and a channel connecting between the plurality of chambers, and that causes the magnetic particles to carry a complex of a test substance and a labelling substance, to detect the test substance on the basis of the labelling substance in the complex. The detection apparatus includes: a rotation mechanism configured to rotate the cartridge about a rotation shaft; a magnet configured to collect the magnetic particles in the chambers; a movement mechanism configured to move the magnet in a direction different from a circumferential direction of a circle in which the rotation shaft is centered; a detector configured to detect the test substance; and a controller programmed to control the rotation mechanism and the movement mechanism so as to transfer the magnetic particles from one of the chambers to another one of the chambers.

A microfluidic device for concentrating particles contained in a fluid sample comprises a substrate (11) having a surface where at least one microfluidic arrangement (M) is defined, which comprises: —a loading chamber (14), for loading the fluid sample into the at least one microfluidic arrangement (M); —a plurality of microchannels (13), which have respective inlet ends connected to the loading chamber (14); and—a covering element (12), which is substantially impermeable to the fluid sample and extends at least partially over the plurality of microchannels (13). The loading chamber (14) and the microchannels (13) extend substantially according to a plane identified by the substrate (11), and the microchannels (13) are partially delimited, in particular at an accumulation region thereof (CA) generally opposite to the respective inlet ends, by filtering means (17) permeable at least to air, the filtering means (17) being configured for withholding within each microchannel (13) any possible particles that may be present in the fluid sample.