This liquid handling device has a first flow passage and a second flow passage. The first flow passage is a flow passage through which first droplets containing a plurality of objects to be sorted can move, and includes a first branching portion. The second flow passage has a droplet dividing portion and a second branching portion. The droplet dividing portion is disposed on the downstream side of the first branching portion, and divides the first droplets containing a prescribed object to be sorted, to generate second droplets containing at most one object to be sorted. The second flow passage is a flow passage through which the second droplets can move. The second droplets can be sorted in the second branching portion. The cross-sectional area of the first flow passage is greater than the cross-sectional area of the second flow passage at an exit of the droplet dividing portion.

A clinical sample storage cassette and an assembly comprising the clinical sample storage cassette is provided. The clinical sample storage cassette can comprise a top layer, a distributor layer, and a storage membrane. The top layer can comprise a first side to receive a clinical sample and a second side coupled with a distributor layer. The distributor layer is to receive the clinical sample from the top layer. The distributor layer can comprise a first distributor side coupled to the second side and a second distributor side coupled to the storage membrane to transfer the clinical sample to the storage membrane. The distributor layer and the storage membrane can have different flow rates of the clinical sample to allow for uniform flow and storage of the clinical sample on the storage membrane.

An example system includes an input channel to flow nucleic segments therethrough, a mixing portion coupled to the input channel, a separation chamber in fluid communication with the second end of the input channel, at least two output channels coupled to the chamber, and an integrated pump to facilitate flow through the separation chamber. The mixing portion is to include at least two different categories of beads having different sizes from each other and having a probe to attach to a corresponding nucleic acid segment. The separation chamber has a passive separation structure including an array of columns spaced apart to facilitate separation of the different categories of beads and attached corresponding nucleic acid segment into at least two flow paths based on a size of the category of the beads. Each output channel is to receive separated categories of beads and attached nucleic acid segments.

A microchip comprises a DNA analysis part configured to analyze DNA, which comprises at least a cell lysis chamber for cell lysis and a DNA extraction chamber which is connected to the cell lysis chamber and configured to extract DNA from lysed cells, and a DNA preservation part which is connected to the DNA analysis part via a flow path and configured to preserve a partial portion of a DNA sample.

This disclosure concerns a method of providing a hydrophobic coating on a microfluidic chip that promotes the discrete flow of at least one liquid. It includes applying the hydrophobic coating onto an area of the microfluidic chip. The disclosure further includes a microfluidic chip that provides discrete flow of at least one liquid.

The present disclosure comprises devices, systems and methods for organizing cells into an array, phenotyping them via image-based analysis over short or long durations, and conducting massively parallel barcoded genomic analysis with DNA barcodes that are present next to each cell.

Microfluid chips that comprise one or more microscale and/or mesoscale condenser arrays, which can facilitate particle purification and/or fractionation, are described herein. In one embodiment, an apparatus can comprise a layer of a microfluidic chip. The layer can comprise an inlet that can receive fluid, an outlet that can output a purified version of the fluid, and a condenser array coupled between and in fluid communication with the inlet and the outlet. The condenser array can comprise a plurality of pillars arranged in a plurality of columns. Also, a pillar gap sized to facilitate a throughput rate of the fluid of greater than or equal to about 1.0 nanoliter per hour can be located between a first pillar of the plurality of pillars in a first column of the plurality of columns and a second pillar of the plurality of pillars in the first column.

A device for forming a liquid aliquot, the device including: a first layer; an elastic second layer overlapping the first layer; a first passageway to receive and hold a volume of liquid, the first passageway formed from the first and second layers; a first actuator to press on the elastic layer thereby dividing the liquid filled passageway into a series of liquid aliquots; a series of vents associated with the series of aliquots; a second actuator to control flow of liquid aliquots through the associated vents; and an attachment structure for attachment of aliquot receptacles to receive liquid aliquots that flow through the vents.

A chip for sample analysis is provided. The chip for sample analysis according to an embodiment of the present application includes a sample storage unit, a capture path configured to capture an analyte contained in a sample and communicating with the sample storage unit, a washing solution storage unit communicating with the capture path and a delay unit included between the capture path and the washing solution storage unit such that a washing solution flowing from the washing solution storage unit to the capture path passes through. When the chip is rotated for sample analysis, the delay unit may be configured to delay the passage of the washing solution introduced from the washing solution storage unit such that the washing solution reaches the capture path later than the sample.

In addition, a cartridge which is mounted on the chip for sample analysis and includes at least one inlet which is inserted into at least one of a sample storage unit, a first eluent storage unit, a second eluent storage unit and a washing solution storage unit of the chip for sample analysis to inject a corresponding solution, is provided.

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.