Described herein are devices and methods for high throughput purification of particles. In some cases, methods and devices described herein can be used to remove erythrocytes and purify leukocytes and raise the quality of umbilical cord blood and other transplant grafts, thereby significantly improving patient outcomes.

A diagnostic cassette includes a substrate, to physically and electrically connect the product to a computing device, a reservoir defined within the substrate to receive a fluid sample for processing by the diagnostic cassette, a reagent to react with the fluid sample deposited in the reservoir to form a solution to enable processing of the fluid sample by the diagnostic cassette, a channel to direct the solution, and a sensor to measure a number of parameters of the solution passing through the channel. A method for measuring microfluidic samples includes receiving, in a reservoir, a fluid sample to be measured, combining the fluid sample with a reagent to create a solution, moving the solution through a channel, and measuring the solution, using sensors, as the solution passes through the channel.

A microfluidic assembly may include a microfluidic chip operably coupled to a device source pressure port and a device relief pressure port, first and second input reservoirs, an output reservoir, and a reservoir interface. The microfluidic chip may include a microfluidic circuit configured to support a fluid flow that includes a gas flow and a liquid flow within the microfluidic circuit. The reservoir interface may be configured to operably couple the first and second input reservoirs to the microfluidic circuit. The device source pressure port may be configured to receive a source pressure to generate the fluid flow through the microfluidic circuit and cause a mixing of liquids to form an output liquid for delivery to the output reservoir via the fluid flow. The first liquid, the second liquid, and the output liquid need not contact the device source pressure port or the device relief pressure port during the mixing.

A system, configured to facilitate processing and detection of nucleic acids, the system comprising a process fluid container and a cartridge comprising: a top layer, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a detection chamber, comprises a portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.

Systems and methods are provided to assist in developing a precision medicine approach using a microfluidic cell having a releasable, aqueous interfacial film to separate tissue channels. The approach can include a personalized medicine treatment plan. The systems and methods emulate cellular communication in a disease state in a more accurate aqueous environment and provide data on the interaction between the cells that can be used to develop a treatment for a subject in need. The systems and methods also can be used to assess the effect of a particular treatment, such as a drug therapy, radiation therapy, or a combination thereof, for example. The systems and methods can show how a particular therapy is affected by any of several known factors including, but not limited to, the sex of the subject, the age of the subject, hereditary factors or other genetic predispositions, as well as perhaps other physiological states of the subject, or a combination thereof.

Diagnostic systems for sensing biological molecules are disclosed. The diagnostic systems include a fluid control delivery and control system that can be coupled to a diagnostic cartridge that includes a microfluidic device and integrated sensing electronics. The diagnostic systems can be used to sequence biological molecules.

Devices, methods and systems are provided for isolating extracellular matrix bodies. More particularly, this invention discloses devices, methods and systems for isolating extracellular matrix bodies from a biological sample for use in diagnosis and prognosis of a subject. A device may have restriction channels for isolating extracellular matrix bodies and uniform flow channels for precise pressure measurements.

A microfluidic system includes a hydrophobic fluidic platform and a heater. The platform includes a plurality of electrode cells operably connected to a voltage source and a controller. The heater is configured to fuse first and second vesicles. The first and second vesicles encapsulate first and second DNA precursors, respectively. The fusing combines the first and second DNA precursors. In another embodiment, a microfluidic system includes a fluidic platform including a plurality of electrode cells, a vesicle mover, and a reaction facilitator. The vesicle mover is configured to move first and second vesicles to a selected cell of the plurality of electrode cells. The reaction facilitator is operably connected to the selected cell. A method includes providing a fluidic platform comprising a plurality of cells; moving first and second vesicles encapsulating first and second reagents, respectively, to a first cell; and fusing the first and second vesicles.

The present disclosure, in some aspects, provides devices useful for determining a target nucleic acid profile of a sample. The device comprises an enrichment module, a reaction module and a nucleic acid sequencer. In other aspects, the present disclosure provides kits, components and compositions (such as consumables) of the devices and methods of using the devices described herein.

Described herein are systems relating to a continuous-flow instrument that includes all necessary components for digital droplet quantification without the need to introduce key reagents or collect and transfer droplets between stages of instrument operation. Digital quantification can proceed without any additional fluid or consumable handling and without exposing fluids to risk of external contamination.