A microchannel chip (1) includes: a first channel (201); a reagent holder (230); a gas inlet (210) communicating with the first channel; a lid (50) closing the gas inlet; and a second channel (202) provided downstream of the first channel. A specimen holder (240) is provided downstream of the reagent holder (230). When the lid (50) closes the gas inlet (210), gas is supplied to the first channel (201) and pushes out the reagent downstream from the reagent holder (230). Thus, the pushed-out reagent merges with a specimen in the second channel (202).

The present disclosure relates to a liquid handling device, methods of operating a liquid handling device, a method of performing a diagnostic test, a computer program and a system. In particular, the liquid handling device is capable of controllable bi-directional or multi-directional flow of reagents across one or more reaction zones allowing rapid, precise and controllable quenching of reactions and/or biological interactions.

Described herein is a microfluidic chip comprising a first channel in fluid communication with an adjacent second channel through a opening, wherein the height of the first channel and the second channel are chosen to generate sufficient surface tension at the opening such that a liquid injected into the first channel or the second channel is substantially confined within the first channel or the second channel, respectively, or that flow of the liquid therebetween is controlled, the surface tension producing a non-physical microfluidic barrier that limits or selectively controls passage of the liquid. Also described are in vitro microphysiological systems that use such microfluidic chips in modeling the structure and functions of human organs, such as a blood-brain barrier, and studying in vivo-like physiological responses of such organs to various investigative or therapeutic agents.

Microfluidic network device (2) configured to supply reagents to a biological tissue sampling device (1), comprising a plurality of microfluidic inlet channels (12) connected to respective sources of said reagents, at least one common outlet channel (22), and a plurality of valves (36) interconnecting an outlet end (14) of each of said plurality of inlet channels to said at least one common outlet channel.

A solution mixer comprising: a main flow path in which a solution circulates; at least one solution introduction flow path connected to the main flow path; and at least one solution discharge flow path connected to the main flow path, wherein the solution discharge flow path has at least one solution discharge flow path valve, and wherein the main flow path has at least one main flow path valve.

According to one embodiment, an automatic analyzing apparatus includes a feeder and a mixer unit. The feeder is configured to feed a first liquid and a second liquid. The mixer unit includes an inflow part, an internal space, and an outflow part. The mixer unit is configured so that the first liquid and the second liquid fed from the feeder enter through the inflow part, the first liquid and the second liquid entering through the inflow part flow inside the internal space, and the first liquid and the second liquid flowing inside the internal space exit through the outflow part according to an inflow entering through the inflow part.

The invention relates to a device and method for in situ temperature-induced antigen retrieval of samples wherein all steps are performed under a pressure higher than the atmospheric pressure on a sample immobilized on a sample support which can be further subjected to staining and imaging on the same sample support, optionally by cycle multiplexing that enables imaging of various molecular targets through multi-molecular read-outs on the same sample in a rapid, highly sensitive and reliable manner.

Limit size lipid nanoparticles, methods for using the lipid nanoparticles, and methods and systems for making limit size lipid nanoparticles.

Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.

The present inventive concept relates to a method for measuring analyte concentration in a sample fluid, comprising: receiving dilution fluid or sample fluid comprising analyte in a microfluidic channel, wherein the dilution fluid or sample fluid further comprises a molecule which is different from the analyte; performing a first affinity-based assay in a first detection zone of the microfluidic channel to measure a signal indicative of the concentration of the molecule in the dilution fluid or sample fluid; mixing the dilution fluid or sample fluid in the microfluidic channel with another of the dilution fluid or sample fluid to obtain a diluted sample fluid; performing a second affinity-based assay in a second detection zone of the microfluidic channel to measure a signal indicative of the concentration of the molecule in the diluted sample fluid; performing a third assay in the second detection zone to measure a signal indicative of the concentration of the analyte in the diluted sample fluid; determining a concentration of the molecule in the received dilution fluid or sample fluid, based on the measured signal of the first affinity-based assay; determining a concentration of the molecule in the diluted sample fluid, based on the measured signal of the second affinity-based assay; and determining the analyte concentration in the sample fluid on basis of the measured signal indicative of the concentration of analyte in the diluted sample fluid and a ratio between the determined concentration of the molecule in the received dilution fluid or sample fluid and the determined concentration of the molecule in the diluted sample fluid. The present inventive concept further relates to a microfluidic arrangement for facilitating measurement of analyte concentration in a sample fluid, and to a system for measuring analyte concentration in a sample fluid, comprising the microfluidic arrangement, and to a diagnostic system comprising the microfluidic arrangement.