Devices and methods for analyzing a sample are disclosed. In various embodiments, the present disclosure provides devices and methods for preparing a serial dilution of a sample. In various embodiments, the present disclosure provides devices and methods for preparing a serial dilution of a sample and conducting sample analysis. In various embodiments, the present disclosure provides a cartridge device and a reader instrument device. The reader instrument device receives, operates, and/or actuates the cartridge device to prepare a serial dilution of a sample and conduct sample analysis.

An example system includes a primary channel having a first end and a second end, at least two reagent reservoirs coupled to the first end, and a controller. Each reservoir contains a reagent in a fluid solution and is associated with an integrated pump to drive a reagent droplet from the corresponding reagent reservoir into the primary channel towards the second end. The controller is coupled to the integrated pumps and operates according to a sequence to actuate the integrated pumps, the sequence being indicative of reagents in the reagent reservoirs. The actuation of the pumps is to drive the reagent droplets from the reagent reservoirs into the primary channel in accordance with the sequence. The example system also includes a shell material reservoir with a shell material and an associated shell material pump to drive the shell material into the primary channel to encapsulate the reagent droplets.

The present application relates to biosensors and methods for detecting and/or quantifying a target analyte such as a target allergen or toxin. In some embodiments, the biosensors use an allergen- or toxin-binding molecule conjugated to a fluorescent label such as a quantum dot that adheres to and is quenched by graphene oxide in the absence of the allergen or toxin.

Disclosed herein are systems and methods for serial flow emulsion processes. Systems and methods as described herein result in reduced cross-contamination.

Disclosed is a microfluidic biosensing system including a processor, in which a Raman barcode database corresponding to at least one Raman spectrum signal is stored, a plurality of Raman barcode beads mixed with a target fluid and coupled to at least one target bioparticle in the target fluid, a microfluidic channel disposed to make the target fluid mixed with the Raman barcode beads flow therethrough, a light source disposed on the microfluidic channel, and a spectral detection device connected to the processor and disposed to correspond to the light source. The spectral detection device receives the Raman spectrum signal generated when the target bioparticle coupled with the Raman barcode bead is irradiated, and transfers the received Raman spectrum signal to the processor. The processor determines a type of the bioparticle(s) and calculates the number of bioparticle(s) by matching the Raman spectrum signal(s) to the Raman barcode database.

Disclosed in the present invention are a digital nucleic acid amplification testing method and an integrated detection system based on CRISPR-Cas technology. The integrated detection system comprises an integrated reaction chip, a temperature control module, a light source and an optical signal detector. The method comprises: uniformly dividing a nucleic acid amplification reagent into amplification micro-droplets, then mixing the amplification micro-droplets after digital nucleic acid amplification with detection micro-droplets containing CRISPR-Cas detection reagent to perform a CRISPR reaction, and when the reaction is finished, detecting an optical signal to realize high-specificity testing of a target object, and the concentration or copy number of nucleic acid molecules in a sample to be tested is also obtained, and high-sensitivity absolute quantitative testing of a target object is realized.

Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel.

The microfluidic devices and systems disclosed herein reduce sample loss and help decrease sample processing bottlenecks for applications such as next generation sequencing (NGS). The microfluidic devices include a plurality of reaction modules. Each reaction module may comprise one or more reaction circuits. Each reaction circuit may comprise a single reaction flow channel with each reaction circuit connected by a bridge flow channel. Alternatively, each reaction circuit may comprise two or more reaction flow channels connected by two or more bridge flow channels. The combination of any two bridge flow channels and a portion of the two or more reaction flow channels between the any two bridge flow channels defining may define the reaction circuit. The reaction module may be arranged as nodes connected by bridge flow channels or each reaction module may be arranged in a parallel fashion on the microfluidic device.

A mixer for generating particles, comprising a first mixing unit, wherein the first mixing unit comprises a first channel (702) and a second channel (701), the first channel (702) comprises a rectilinear channel, the second channel (701) comprises a curvilinear channel. The mixer is particularly suitable for producing nanoparticles, and the mixing efficiency can be improved. A microfluidic hybrid chip cartridge prepared by the mixer is also provided.

The present invention relates to fluidic devices for preparing, processing, storing, preserving, and/or analyzing samples. In particular, the devices and related systems and methods allow for preparing and/or analyzing samples (e.g., biospecimen samples) by using one or more of capture regions and/or automated analysis.