A cell processing system, fluidics cartridge, and methods for using actuated surface-attached posts for processing cells are disclosed. Particularly, the cell processing system includes a fluidics cartridge and a control instrument. The fluidics cartridge includes a cell processing chamber that has a micropost array therein, a sample reservoir and a wash reservoir that supply the cell processing chamber, and a waste reservoir and an eluent reservoir at the output of the cell processing chamber. A micropost actuation mechanism and a cell counting mechanism are provided in close proximity to the cell processing chamber. A method is provided of using the cell processing system to collect, wash, and recover cells. Another method is provided of using the cell processing system to collect, wash, count, and recover cells at a predetermined cell density.

To provide a technique capable of forming stable droplets.

There is provided a microparticle sorting device including a microchip including a main flow path through which a liquid containing a microparticle flows, a sheath liquid flow path that communicates with the main flow path and through which a sheath liquid flows, and a sheath liquid introduction portion that introduces the sheath liquid, in which the sheath liquid flowing through the sheath liquid introduction portion is vibrated. Furthermore, there is also provided a microparticle sorting method including, in a microchip including at least a main flow path through which a liquid containing a microparticle flows, a sheath liquid flow path that communicates with the main flow path and through which a sheath liquid flows, and a sheath liquid introduction portion that introduces the sheath liquid, vibrating the sheath liquid flowing through the sheath liquid introduction portion.

A method for monitoring a reaction and a reaction system are provided. The reaction system includes at least one vessel for the reaction medium, which is in fluid communication with an injection tube; at least one vessel for a carrier fluid that is immiscible with the reaction medium, which is in fluid communication with a reaction tube; the injection tube being mounted so as to lead into the reaction tube such that individual drops of the reaction medium can be injected into the reaction tube and into the immiscible carrier fluid, so as to form a train of reaction chambers; at least one detector for monitoring a reaction; a means for classifying the reaction chambers; and at least one means for recirculating reaction chambers in front of at least one detector for monitoring a reaction.

In one example in accordance with the present disclosure, a cellular analytic system is described. The cellular analytic system includes an analytic device. The analytic device includes a chamber to receive a cell to be analyzed. At least one lysing element agitates the cell and at least one sensor detects a change in the cell based on an agitation of the cell. The cellular analytic system also includes a controller to determine a rupture threshold of the cell based on parameters of the agitation when a cell membrane ruptures.

Microfluidic device for isolating a microparticle from a heterogeneous sample includes a first microfluidic chamber containing a first chamber inlet; a plurality of first chamber outlets in fluid connection with the first chamber inlet; and a loop. The microfluidic device further contains a second microfluidic chamber containing a second chamber inlet and a plurality of second chamber outlets in fluid connection with the second chamber inlet. The second microfluidic chamber contains a loop. In some embodiments, the first and second microfluidic chambers include from about 1 loop to about 50 loops; or from about 2 loops to about 25 loops; or from about 5 loops to about 15 loops. A first chamber outlet or a plurality of first chamber outlets is in fluid connection with the second chamber inlet. A method for removing a microparticle from a heterogeneous sample, and a water purification system and method use the microfluidic device.

An example method, consistent with the present disclosure, includes receiving in a microfluidic channel, a fluid sample and a reagent, where the reagent includes a reporter nucleic acid labeled with a detectable ligand. The method further includes identifying a target nucleic acid in the fluid sample using a guide ribonucleic acid (gRNA) and a programmable nuclease immobilized in a side channel fluidically coupled to the microfluidic channel. Responsive to identifying the target nucleic acid in the fluid sample, the method includes causing cleavage of the detectable ligand, and detecting the detectable ligand from the cleaved reporter nucleic acid in the side channel.

An assay device includes a colorimetric testing assembly including a detection area, a fluid inlet, and a microfluidic network including a first path extending to the detection area and a second path extending to the detection area. When a fluid (e.g., a buffer fluid or a combined buffer and sample solution) is provided to the fluid inlet, a first portion of the fluid rehydrates a first dried reagent (e.g., a dried enzyme label) disposed along the first path to produce a first rehydrated reagent and a second portion of the fluid rehydrates a second dried reagent (e.g., a dried substrate) to produce a second rehydrated reagent. The first rehydrated reagent and the second rehydrated reagent are then sequentially delivered to the detection area by capillary-driven flow to perform the assay.

Disclosed is a method for assessing a characteristic of a drug, comprising treating a tissue sample with the drug; extracting from the tissue sample at least one feature of the tissue sample as treated; providing the at least one feature to an engine; obtaining a prediction from the engine; and associating the prediction with the drug.

An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.

An apparatus for microfiltration and a scalable method for manufacture of an inertial microfluidic device for such microfiltration apparatus are provided. The apparatus for microfiltration includes one or more inertial microfluidic devices, each including a plurality of spirals of a microfluidic channel. At least one of the inertial microfluidic devices is configured to utilize outer wall focusing for high volume fraction microfiltration of particles. The scalable method for manufacture of the inertial microfluidic device includes micromachining on a polycarbonate-based substrate a rectangular spiral microchannel having one or more input channels and a plurality of output channels configured to utilize high volume fraction outer wall focusing for microfiltration of particles.