An example device includes a microfluidic channel and a movable element retained in the microfluidic channel to move from a first position to a second position by fluid flow through the microfluidic channel. The device includes a sensor to take a sensor reading to determine fluid flow through the microfluidic channel. The device includes a microfluidic pump to return the movable element from the second position to the first position. The device includes a controller to actuate the microfluidic pump and to determine a flow rate of the fluid flow through the microfluidic channel based on the sensor reading.

The present invention relates to a platelet testing device using blockage phenomenon, comprising: a sample chamber containing blood sample; a microfluidic tube which is in fluid communication with the sample chamber and through which the blood sample flows; and a microbead packing arranged on a flow path of the blood sample of the microfluidic tube; wherein the microbead packing comprises: a packing pipe which constitutes a part of the flow path of the blood sample; and a plurality of microbeads contained in the packing pipe and arranged to be in close contact with each other so as to form voids between the microbeads, whereby function of the platelet is tested by blockage phenomenon of the voids due to the platelet in the blood sample which flows through the microfluidic tube from the sample chamber according to the present invention.

A reaction processor is provided with a reaction processing vessel in which a channel is formed, a liquid feeding system, a temperature control system for providing a high temperature region and a low temperature region to the channel, and a fluorescence detector for detecting the sample passing through a fluorescence detection region of the channel, and a CPU for controlling the liquid feeding system based on a signal that is detected. A target stop position X.sup.[L].sub.0(n+1) of the sample in the low temperature region in an (n+1)th cycle is corrected from a target stop position X.sup.[L].sub.0(n) of the sample in the low temperature region in the nth cycle based on the result of stopping control on the sample in the nth cycle.

A sensing device includes a sample loading chamber configured to receive a sample, a detection antibody drying or lyophilization chamber configured to receive a first portion of the sample, one or more substrate drying or lyophilization chambers configured to receive a second portion of the sample, and one or more reaction chambers connected to the detection antibody drying or lyophilization chamber and the one or more substrate drying or lyophilization chambers. The detection antibody drying or lyophilization chamber and one or more substrate drying or lyophilization chambers are placed in parallel between the sample loading chamber and the one or more reaction chambers.

A method for achieving microfluidic perfusion of a spheroid, the method being implemented in a microfluidic device that includes a cavity for hydrodynamically trapping the spheroid, the method including performing a first injection of a gel containing the spheroid into the microfluidic network, hydrodynamically trapping the spheroid in the trapping cavity of the microfluidic network, performing a second injection of a fluid that is non-miscible with the gel into the microfluidic network with a view to flushing away gel present in the network, except in the trapping cavity, cross-linking the gel present around the spheroid, in the trapping cavity, performing a third injection of a culture medium into the microfluidic network with a view to perfusing the spheroid petrified in its gelled environment, and located in the trapping cavity.

Devices and methods for analysing extracellular vesicle glycans are described. According to an embodiment, a microfluidic device comprises an inlet portion configured to receive a fluid sample; a mixing portion fluidically coupled to the inlet portion and configured to facilitate mixing between the fluid sample and magnetic nanoparticles functionalized to bind with extracellular vesicles and aggregate to vesicle glycans in the fluid sample; a magnetic separation portion fluidically coupled to the mixing portion and configured to separate clusters of magnetic nanoparticles from the fluid sample; and a magnetic sensor configured to measure magnetic properties of the fluid sample after it has passed through the magnetic separation portion. The magnetic nanoparticles may configured to aggregate in the presence of respective lectins when bound with extracellular vesicles carrying target glycans. In a specific embodiment, the magnetic particles comprise a magnetic polycore coated with polydopamine.

A method is for detecting a biomarker within a sample of blood. The method may include processing the sample of blood with a microfluidic blood plasma separator and a plasmonic array biosensor, and flowing the sample of blood over a sensing surface of the plasmonic array biosensor. The sensing surface of the plasmonic array biosensor may have an ssDNA aptamer against the biomarker. The method may further include binding the biomarker in the sample of blood to the ssDNA aptamer of the plasmonic array biosensor, and detecting the biomarker in the sample of blood based upon LSPR altering a reflected optical signal from the plasmonic array biosensor.

The present invention relates generally to an assay for detecting and differentiating single or multiple analytes, if present, in a fluid sample, including devices and methods of use of the same.

A drug screening platform simulating hyperthermic intraperitoneal chemotherapy including a dielectrophoresis system, a microfluidic chip and a heating system is disclosed. The dielectrophoresis system is used to provide a dielectrophoresis force. The microfluidic chip includes a cell culture array and observation module and a drug mixing module. The cell culture array and observation module are used to arrange the cells into a three-dimensional structure through the dielectrophoresis force to construct a three-dimensional tumor microenvironment. The drug mixing module is coupled to the cell culture array and observation module and used to automatically split and mix the inputted drugs and output the drug combinations into the cell culture array and observation module. The heating system is used for real-time temperature sensing and heating control of the drug combinations on the microfluidic chip to simulate high-temperature drug environment when performing hyperthermic intraperitoneal chemotherapy on the three-dimensional tumor microenvironment.

An apparatus includes a substrate, a first heating element, and a second heating element. The substrate includes a first portion, a second portion, and a third portion that is between the first portion and the second portion. The first portion is characterized by a first thermal conductivity, the second portion is characterized by a second thermal conductivity, and the third portion is characterized by a third thermal conductivity. The third thermal conductivity is less than the first thermal conductivity and the second thermal conductivity. The first heating element is coupled to the first portion of the substrate, and is configured to produce a first thermal output. The second heating element is coupled to the second portion of the substrate, and configured to produce a second thermal output. The second thermal output is different from the first thermal output.