Disclosed are methods, systems, and articles of manufacture for performing a process on biological samples. An analysis of biological samples in multiple regions of interest in a microfluidic device and a timeline correlated with the analysis may be identified. One or more region-of-interest types for the multiple regions of interest may be determined; and multiple characteristics may be determined for the biological samples based at least in part upon the one or more region-of-interest types. Associated data that respectively correspond to the multiple regions of interest in a user interface for at least a portion of the biological samples in the user interface based at least in part upon the multiple identifiers and the timeline. A count of the biological samples in a region of interest may be determined based at least in part upon a class or type of data using a convolutional neural network (CNN).

Disclosed are a biosensing test strip (100, 200, 300, 500, 600, 700, 800, 900, 1000, 1100) and a biosensing test method. The biosensing test strip (100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100) comprises: a reaction layer (120, 220, 720, 820) provided with a reaction flow channel (121, 221, 821, 920, 1020); a partition plate layer (130, 230) located above the reaction layer (120, 220, 720, 820) and covering the reaction flow channel (121, 221, 821, 920, 1020); an exhaust layer (140, 240, 540, 640) located above the partition plate layer (130, 230), with the exhaust layer (140, 240, 540, 640) being provided with an exhaust flow channel (141, 241, 550, 650); and a communication hole passing through the partition plate layer (130, 230) to enable the exhaust flow channel (141, 241, 550, 650) to be in communication with the reaction flow channel (121, 221, 821, 920, 1020).

An analytical toilet is disclosed with a bowl adapted to receive excreta, a conduit for transporting a liquid excreta sample from the bowl, and a liquid reagent source. The analytical toilet also includes a microfluidic chip that has a sensor configured to detect at least one property of the excreta sample. The microfluidic chip also has an excreta sample path in fluid communication with the conduit and the sensor and a reagent path in fluid communication with the liquid reagent source and the sensor. The length of and number of channels in the sample path and the reagent path are selected so as to control the respective fluid resistance of the excreta sample and the reagent to thereby optimize the mixing and flow rates of the excreta sample and reagent into the sensor. There is also disclosed analytical toilet with a microfluidic chip having reagent path that includes a first and a second channel. The second channel is longer than the first channel. A valve, which is controllable so as to cause the reagent to flow through either the first channel, the second channel or both channels. As such, the fluid resistance of the reagent is controlled, to thereby optimize the flow rate of the reagent into the sensor.

Provided is an apparatus for inducing and/or controlling flow of a fluid within a microchannel in a microfluidic device. The apparatus includes a fluid reservoir configured for holding a volume of fluid to be transported through said microfluidic channel and also configured for fluid connection to an inlet of said microfluidic channel. The apparatus also includes an evaporation reservoir configured for fluid connection to an outlet of said microfluidic channel. The evaporation reservoir includes at least one wetting, wicking or hydrophilic structure positioned at least partly within the reservoir. The wetting, wicking, or hydrophilic structure is capable of absorbing or conducting a fluid present in the microfluidic channel via wicking action or capillary force and maintaining a substantially constant volume of fluid in the evaporation reservoir. In use, evaporation of fluid at the outlet results in fluid being drawn from the fluid reservoir through the microfluidic channel to thereby create a flow of the fluid in the microfluidic channel.

There is provided a microfluidic chip for sensing an analyte in a sample by colorimetry. The microfluidic chip comprises: an inlet adapted to receive the sample; an incubation chamber having an incubation chamber inlet fluidly connected to the inlet downstream thereof, to incubate the analyte in the sample; a filter barrier fluidly connected to the incubation chamber, downstream of the incubation chamber inlet; a sensing chamber fluidly connected to the incubation chamber, downstream of the filter barrier, the sensing chamber having a plasmonic nanosurface, the plasmonic nanosurface including nanostructures protruding from the plasmonic nanosurface, the nanostructures having a size that is smaller than that of the diffraction limit of light, the nanostructures having a metallic layer that is plasmon-supported on top of a back reflector layer; and an outlet fluidly connected to the sensing chamber downstream thereof.

A fluid device includes a substrate and a gas-liquid separating filter, the substrate has a flow path through which a solution flows, a reservoir, in which the solution is accommodated, connected to the flow path, an injection hole configured to connect the reservoir to the outside, and an air introduction hole branched off from the injection hole and connected to the outside, and the gas-liquid separating filter is disposed in a path of the air introduction hole, allows passage of a gas flowing through the air introduction hole, and prevents passage of a liquid flowing through the air introduction hole.

The present disclosure relates to a consumable sample partition device and it assembly and use. The sample partition device can be used to test a sample for absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). The sample partition device partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.

Devices, systems, and methods for trapping and manipulating portions of tissue are described. In an embodiment, the devices include an array of traps, wherein traps of the array of traps are shaped to trap a tissue sample; and a well is in registry and fluidic communication with a trap of the array of traps.

The present disclosure relates to a microfluidic device for measuring one or more parameters in a fluid sample, which includes a sample microfluidic channel disposed on a solid substrate, a reagent microfluidic channel disposed on a solid substrate, a mixing microfluidic channel disposed on a solid substrate, and an optical reading window located downstream of the mixing microfluidic channel, through which a response indicative of the parameter(s) change can be measured optically. The present disclosure also relates to an apparatus for measuring one or more parameters in a fluid sample which includes the microfluidic device as well as a method for measuring one or more parameters in a fluid sample through the device or the apparatus.

In an embodiment, the present disclosure pertains to a microfluidic platform composed of a droplet generator having an entry point for donor particles and target particles, a first droplet incubation chamber in fluid communication with the droplet generator, a droplet detection functionality to allow for analysis of the inner content of droplets, and a droplet sorting functionality to allow for the separation of droplets based on the analysis of the inner content of droplets. In another embodiment, the present disclosure pertains to a method for cell-to-cell DNA, RNA, or other genetic material transfer through use of a water-in-oil emulsion microdroplet-based microfluidic platform for automation and high throughput identification or screening of genetic transfer outcomes utilizing the microfluidic platforms as disclosed herein.