A detection chip, a method for manufacturing a detection chip, a method for operating a detection chip, and a reaction system are disclosed. The detection chip includes a first substrate, a micro-cavity definition layer, and a heating electrode. The micro-cavity definition layer defines a plurality of micro-reaction chambers. The heating electrode is configured to release heat after being energized. The heating electrode includes a first electrode portion and at least one second electrode portion. Orthographic projections of the plurality of micro-reaction chambers on the first substrate are within an orthographic projection of the first electrode portion on the first substrate, the orthographic projections of the plurality of micro-reaction chambers on the first substrate do not overlap with an orthographic projection of the second electrode portion on the first substrate, and a resistance value of the first electrode portion is greater than a resistance value of the second electrode portion.

The present invention relates to a system which permits securing an electronic label on a test tube. In particular, it refers to an Internet of Things (IoT) based platform for real-time remote sensing and monitoring of specimen transportation and banking. The technology is based on an interconnected smart collecting tubes and transportation box monitored with a remote digital interface, allowing real-time sample monitoring of key parameters such as sample identification, temperature, volume, geolocalization, sealing and bio-banking.

Provided are devices, methods, and systems for temperature control of individual containers in a thermocycler for polynucleotide synthesis. Provided herein are devices, methods, and systems comprising a circuit patch having a heating element that is placed over a reaction container on a lid of the reaction container or directly over the reaction container Provided herein are devices, methods, and systems comprising a single-piece sensor assembly for a thermistor plate assembly comprising a sensor holder having a sensor pad that is in contact with the container holder.

Improved sub-assemblies and methods of control for use in a diagnostic assay system adapted to receive an assay cartridge are provided herein. Such sub-assemblies include: a brushless DC motor, a door opening/closing mechanism and cartridge loading mechanism, a syringe and valve drive mechanism assembly, a sonication horn, a thermal control device and optical detection/excitation device. Such systems can further include a communications unit configured to wirelessly communicate with a mobile device of a user so as to receive a user input relating to functionality of the system with respect to an assay cartridge received therein and relaying a diagnostic result relating to the assay cartridge to the mobile device.

Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.

A method of conducting a biological assay, comprises obtaining data corelative to a temperature of a reagent, mixing the reagent with a sample to provide a mixture, receiving from the mixture a signal indicative of an amount of an analyte in the sample, and correcting the amount based on the obtained data and on a type of the reagent.

The invention refers to a method of detecting nucleic acid sequences in biological samples from medical, agricultural and biotechnological sources, and a corresponding device, that can be used in health care, in particular in laboratorial diagnosis, to detect genetic sequences, with the objective of identifying viruses and diseases arising from genetic malformations, bringing novelties of using saliva samples, making extraction of RNA from the genetic material from the sample, through a small device with low complexity and innovative design, with the advantages of portable, rapid results, with on-line connectivity to a test results center, dispensing frequent visits to the doctor, hastening the start of treatment, allowing access for groups of people and eliminating the need for a highly qualified operator.

A method to monitor and control the temperature of a sample holder of a laboratory instrument during execution of a temperature profile on the sample holder is presented. The laboratory instrument comprises a sample holder with high temperature uniformity and at least three identical temperature sensors. The measured actual temperatures of the sample holder are processed in order to determine if the execution of the temperature profile should be continued or aborted. Furthermore, temperature sensors which measure actual temperatures that do not fulfil certain requirements are excluded from further monitoring and controlling the temperature of a sample holder.

A power-compensated fusion furnace includes a power control system having one switching device per heating element, power measurement circuitry, a master temperature sensor, and a controller. Each switching device is electrically connected to a respective heating element. The controller, in conjunction with the switching devices, is able to individually control the electrical energy flowing to each heating element, thereby controlling the duty cycle of each heating element. The duty cycles are corrected for one or more of: variations in the electrical resistance of each heating element and position-dependent variations in furnace cavity temperature.

A solid reagent containment unit is formed by a support; a frame body fixed to the support and delimiting internally, together with the support, an analysis volume; a reagent-adhesion structure within the analysis volume; and at least one reagent cavity, which extends within the reagent-adhesion structure. The reagent-adhesion structure is of an adhesion material embossable at temperatures lower by 6-8° C. than its own melting point and has a melting point such as not to interfere with the analysis. The reagent cavity forms a retention wall, laterally surrounding the reagent cavity, and houses dried reagents. The adhesion material is chosen among wax, such as paraffin, a polymer, such as polycaprolactone, a solid fat, such as cocoa butter, and a gel, such as hydrogel or organogel.