An analytic device comprising a device housing, a dock to receive a camera enabled mobile electronic device, such as a smartphone and other smart devices, and a processing device to communicate with the mobile electronic device and to control a condition of the assay tube, such as temperature. In another example, the analytic device comprises a device housing and a circuit board. A processing device, a heating block defining a recess to support assay tube, and a resistive heater are surface mounted to the circuit board. A light source and a fan are also provided. A dock may be provided to support a mobile electronic device. The mobile electronic device communicates with the processing device to cause the application of reaction conditions to the assay tube, to perform a PCR procedure, for example. Methods are also disclosed.

The present disclosure provides a digital PCR system. The system includes a droplet formation assembly and a droplet orifice assembly. The droplet formation assembly includes a heat conducting plate and a cover plate, at least one inverted U-shaped step is placed on a side surface of the cover plate, the heat conducting plate, the cover plate and the inverted U-shaped step together form a droplet formation chamber having an opening at a bottom. The droplet orifice assembly is connected below the droplet formation assembly, and includes a plurality of droplet orifices, the droplet orifice is connected with the droplet formation chamber, and a vaporization component is placed in the droplet orifice, the vaporization component vaporizes a digital PCR solution in the droplet orifice and rapidly pushes the digital PCR solution into a droplet forming oil in the droplet formation chamber, to form a digital PCR droplet.

The invention provides a reciprocal-flow-type nucleic acid amplification method performing thermal cycling by reciprocating a sample liquid between a denaturation temperature zone and an elongation-annealing temperature zone with a connected microchannel including at least a curved channel corresponding to the denaturation temperature zone, a curved channel corresponding to the elongation-annealing temperature zone, a linear or curved intermediate channel that connects the aforementioned curved channels, and a connector to connect to a liquid delivery mechanism for enabling movement of the sample liquid. The method includes moving the sample liquid in the channel by the liquid delivery mechanism that is open to atmospheric pressure when liquid delivery is stopped, and measuring a fluorescence intensity for each thermal cycle at a predetermined point on the channel corresponding to the denaturation temperature zone and at a predetermined point on the channel corresponding to the elongation-annealing temperature zone to perform real-time PCR.

A microfluidic system for separating biological entities includes a cooling device including a thermoelectric heat pump, a first fan, and a first heat exchanger disposed between the first fan and the thermoelectric heat pump; a first housing structure having a first shell that encases the first fan and the first heat exchanger; a microfluidic device and one or more piezoelectric transducers attached thereto; and a second housing structure reversibly attached to the first housing structure and having a second shell that encloses therein the microfluidic device and the one or more piezoelectric transducers. When the first and second housing structures are coupled, a first air passage is formed between a side of the first heat exchanger and an end of the microfluidic device, a second air passage is formed between the first fan and the piezoelectric transducers, thereby allowing air to circulate between the first and second air passages.

Micro-biological colony counters and more particularly, systems and methods for feeding, identifying, counting, classifying, segregating and collecting organic samples such as but not limited to micro-organisms. The system can classify colonies in different classes such as bacteria and fungus present in an organic sample and also gives a digital count on the number of colonies present in each of the classes separately. The system reduces the time in counting and classification of microbes in each class separately, eliminates manual errors and requires less manual intervention. The system is reliable and can perform the counting and classification of microbes in each class separately even in absence of well-trained technician. The system can count surface colonies in petri-dish and can count colonies in different size or diameter of petri-dish.

The present disclosure relates to the field of liquid handling and dispensing systems in combination with additive manufacturing. In particular, it relates to temperature-controlled units, i.e. dispensing heads and source well holders, for receiving, holding and releasing liquid and semi-liquid material, liquid-handling and dispensing systems, apparatuses and methods for applying temperature-sensitive liquids. A temperature-controlled unit (1) comprises at least one Peltier element (3), each Peltier element having opposite first and second surfaces (4a, 4b). The unit (1) further comprises at least one cooling element (5). The at least one Peltier element (3) is arranged to have each respective first surface (4a) facing a reservoir block (2) of the unit (1). The at least one cooling element (5) is thermally connected to the Peltier element (3) and arranged to transfer heat generated by the at least one Peltier element (3) and dissipate the transferred heat away from the at least one Peltier element (3).

The invention(s) cover systems and methods for target detection in a multiplexed and rapid manner. Embodiments of the system can include: a base substrate; and an array of sample processing regions defined at a broad surface of the base substrate, wherein each of the array of sample processing regions includes: a set of microwell subarrays arranged in a gradient by volumetric capacity between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions. The system can support methods, with example implementation by an automated platform, for returning preliminary results from a subset of microwells of the samples processing regions, as well as results pertaining to specific and non-specific amplification, for multiple targets of a sample.

A generic point of care based portable device and method thereof as a platform technology for detecting pathogenic infection via nucleic acid based testing achieving sample-to-result integration, comprising the following interconnected stand-alone modules: a thermal unit for executing piece-wise isothermal reactions in a pre-programmable concomitant fashion without necessitating in-between operative intervention; a colorimetric detection unit seamlessly interfaced with smartphone-app based analytics for detecting the target analyte. The said platform technology is thus capable of detecting targeted pathogen-associated RNA by coupling additional complementary DNA probe hybridization combined with isothermal reaction purposed for reverse transcription of RNA followed by amplification of the resulting c-DNA as well as subsequent specific binding of the same in a single user-step in a concomitant fashion and its smartphone-enabled interpretation, in a generic modular format that renders operative suitability outside controlled laboratory environment in a user-friendly manner, with predictive accuracy favorably comparable with gold standard RT-PCR tests.

A sample temperature adjustment device includes a temperature control space for controlling a temperature of a sample by containing the sample inside, a heat insulating layer enclosing the temperature control space so that the temperature control space is thermally separated from outside air of the sample temperature adjustment device, a cooling part for cooling the temperature control space, a heat transfer plate covering at least a part of outer surfaces of the heat insulating layer, and a heater that heats the heat transfer plate to prevent dew condensation on a surface of the heat transfer plate.

A thermal cycler for DNA amplification and real-time detection comprises a sample block with a position for a sample tube containing fluorescent molecules; an LED light for excitation of the fluorescent molecules at a predetermined wavelength; a filter that passes light at the predetermined wavelength to allow excitation of the fluorescent molecules; and a housing for the sample block, the LED light, and the filter that allows visualization of the fluorescent signal. The device may utilize a smart phone with a camera or the naked eye to detect fluorescence.