Methods and devices for evaluating coagulation are described, including methods and devices for detecting an anticoagulant agent or a coagulation abnormality. In various embodiments, the methods and devices of the invention measure coagulation of a sample in response to a gradient of one or more coagulation factors. These responses can be evaluated to accurately profile coagulation impairments of the sample, including the presence of anticoagulant medication. In various embodiments, the invention provides point-of-care or bedside testing with a convenient, microfluidic device that can be used by minimally trained personnel.

The invention relates in a first aspect to an improved fluidic device for determining a property of a microbe. The device comprising a bottom layer comprising a plurality of light-transmissive wells; and a top layer comprising an injection opening and a fluid distribution system. Each of said plurality of distribution channels has: essentially the same channel length between the inlet end and the outlet end; and essentially the same channel volume. The invention relates in a second aspect to a use of the device for determining a susceptibility of a microbe, preferably a bacterium, to an antimicrobial drug.

A lateral-flow assay device includes a substrate having a sample addition zone and a wash addition zone downstream thereof along a fluid flow path through which a sample flows. The fluid flow path is configured to receive a wash fluid in the wash addition zone. A hydrophilic surface is arranged in the wash addition zone. Flow constriction(s) are spaced apart from the fluid flow path and arranged to define, with the hydrophilic surface, a reservoir configured to retain the wash fluid by formation of a meniscus between the hydrophilic surface and the flow constriction(s). The fluid flow path draws the wash fluid from the reservoir by capillary pressure. Apparatus for analyzing a fluidic sample and methods of displacing a fluidic sample in a fluid flow path of an assay device are also described.

Disclosed herein are systems and methods for detecting ocular analytes in vitreous humor or aqueous humor. Specifically exemplified are systems having a sample acquisition device that is inline with an analyte detection device. The system embodiments allow for the easy procurement and testing of samples. In a typical embodiment, the analyte detection device includes a sample staging chamber and a test chamber that comprises reagents that specifically interact with the analyte. The test chamber may include a sample pad, a conjugate pad having at least one conjugate reagent specific to the analyte loaded thereon, an assay platform having a substrate with at least one test region having a test reagent immobilized thereon, the test reagent being specific to the analyte; and an optional absorbent pad.

The present disclosure relates to methods, devices, assays and systems for rapid detection of food-borne pathogens, including Vibrios.

A sample analysis substrate includes a substrate; a first holding chamber; a reaction chamber; a first flow path having a first opening and a second opening respectively connected with the first holding chamber and reaction chamber; a main chamber; a second flow path having a third opening and a fourth opening respectively connected with the reaction chamber and the main chamber; and a magnet accommodation chamber capable of accommodating a magnet. The first opening is located closer to a rotation shaft than the second opening. The second opening is located closer to the rotation shaft than the third opening. The magnet accommodation chamber is located at a position at which, in the case where the magnet is accommodated in the magnet accommodation chamber, the magnet captures magnetic particles in the main chamber. The sample analysis substrate is rotatable to transfer a liquid.

A microfluidic device in which microfluidic channels are embedded in a culture medium chamber and have open sides. The microfluidic device is patterned with a fluid moved along a hydrophilic surface due to capillary force, and the fluid may be rapidly and uniformly patterned along an inner corner path and a microfluidic channel. In the microfluidic device, the microfluidic channel is connected to facilitate fluid flow with a culture medium through open sides thereof and openings, and thus may provide a cell culture environment in which high gas saturation is maintained. In addition, several microfluidic devices formed on one common substrate are described. Such microfluidic devices may be manufactured of a hydrophilic engineering plastic by injection molding.

Microfluidic systems and methods are described for capturing magnetic target entities bound to one or more magnetic beads. The systems include a well array device that includes a substrate with a surface that has a plurality of wells arranged in one or more arrays on the surface. A first array of wells is arranged adjacent to a first location on the surface. A second and subsequent arrays, if present, are arranged sequentially on the surface at second and subsequent locations. When a liquid sample is added onto the substrate and caused to flow, the liquid sample will flow across the first array first and then flow across the second and subsequent arrays in sequential order. The wells in the first array each have a size that permits entry of only one target entity into the well and each well in the first array has approximately the same size.

The present disclosure relates to a cell extraction device comprising: a plurality of cell extraction modules arranged in a single layer array, the single layer array having a cell-receiving side wherein an opening of at least one of the plurality of cell extraction modules on the cell-receiving surface is configured to receive and retain a single target cell from a fluid sample, and a fluid-evacuating side wherein an opening of the or each of the plurality of cell extraction modules on the fluid-evacuating side is configured to allow fluid from the fluid sample to be evacuated from the cell extraction device, wherein at least one of the plurality of cell extraction modules comprises a micropore capillary configured at a first end that is open on the cell-receiving side to receive a target cell, and configured at a second end that is open on the fluid-evacuating side to allow fluid to pass through.

The present invention relates to the use of surfaces that exhibit different surface energies wherein the difference in surface energies is configured to disrupt capillary laminar flow of a fluid travelling between the two surfaces. The invention further relates to the use of such surfaces in assay methods including a device utilising same.