The present invention provides a microfluidic system, method and kit for performing assays. The system may comprise a microfluidic device and a detector, wherein the assay yields results that may be read by a detector and analyzed by the system. The assay may comprise one or more chemical or biological reaction against, or performed on, a sample or multiple samples. The sample(s) may become larger and/or smaller during the performance of the assay. The sample(s) may be present within a vehicle, or on a carrier within a vehicle, in the microfluidic device, and wherein the vehicle may become larger and/or smaller during the performance of the assay. The assay may be a cascading assay comprising a series of multiple assays, wherein each assay may be the same or different, and wherein each assay in the series of multiple assays may further comprise one or more process or step.

Disclosed is a liquid discharging nozzle, including a needle stem having a hollow chamber and an outlet end located at one end of the needle stem, an angle between a normal line of an end surface of the outlet end of the liquid discharging nozzle and an extension direction of the needle stem is equal to or smaller than 90°. Further disclosed are a motion controlling mechanism, a microdroplet generating device and method, a liquid driving mechanism and method, a microdroplet generating method, and a surface processing method of a liquid discharging nozzle.

There is provided a droplet sorting device including a detector configured to detect a state of droplets ejected from an orifice that generates a fluid stream and of satellite droplets existing between the droplets, and a controller configured to control a frequency of a driving voltage supplied to a vibration element that applies vibration to the orifice on the basis of positions where the satellite droplets exist.

The present invention provides a microfluidic device which comprises a drive module and a microfluidic platform. The drive module further comprises a rotary unit and a vibration unit for driving the microfluidic platform, and the microfluidic platform further comprises multiple microfluidic elements for performing tests. The present invention also provides a method for operating a microfluidic device. The method comprises steps using the rotary unit and steps using the vibration unit to distribute sample in a microfluidic structure.

Disclosed in the present application is a sample adding needle for preparing microdroplets, comprising a liquid storage portion and a liquid discharge portion, which are integrally injection molded and penetrate one another; the liquid storage portion is a truncated cone the radial dimension of which gradually decreases in the direction facing the liquid discharge portion, and the liquid discharge portion is a truncated cone the radial dimension of which gradually decreases in the direction away from the liquid storage portion; the taper of the liquid storage portion is C1, the taper of the liquid discharge portion is C2, and C1≤C2; the wall thickness of the liquid storage portion is D1, the wall thickness of the liquid discharge portion is D2, and D1>D2. For the sample adding needle for preparing microdroplets as described in the present application, when preparing microdroplets by using the sample adding needle, the sample adding needle performs periodic reciprocating motion at varying speeds in an oily liquid such that a sample solution is subject to periodic shear force from the oily liquid at a liquid discharge opening, thereby enabling the sample solution within the sample adding needle to enter the oily liquid, thus achieving the production of microdroplets having uniform size and controllable volume.

This invention provides an assay device for testing an analyte in samples, comprising a chamber with an opening for collecting samples, a test element, and a sound indication structure. The indication structure creates sound by vibrating after the structure deforms and would move back to the original position. The assay device is easy to operate for non-professionals.

A droplet generating apparatus, system, and method are disclosed. Based on a relative vibration between a micro-pipe and a container containing a second liquid, a first liquid flowing out from an outlet end of the micro-pipe can be detached from the micro-pipe by a fluid shear force of the second liquid to form droplets in the second liquid. Multitudinous quantitative and uniform droplets can be generated precisely and accurately in the present disclosure.

A unitary, molded fluid reservoir body to which a fluid ejection head substrate is attached. The unitary, molded fluid reservoir body includes one or more discrete fluid chambers therein. Each of the one or more fluid chambers have an open top, side walls, and sloped bottom walls attached to the side walls, wherein each of the one or more fluid chambers terminates in a fluid supply via, and wherein the sloped bottom walls have an angle ranging from about 6 to about 12 degrees relative to a plane orthogonal to the sidewalls. An ejection head support face is disposed opposite the open top for attachment of a single fluid ejection device to the ejection head support face for ejecting fluid provided from the one or more chambers through the one or more fluid supply vias.

Provided is a microfluidic system comprising a microfluidic chip including a substrate having a vibrating section that, when irradiated by light, causes at least a portion of a substrate to vibrate, and at least one microfluidic structure arranged adjacent to the vibrating section such that vibration of the at least a portion of the substrate causes a vibration stimulus within the at least one microfluidic structure, the vibration stimulus causing a change in position of at least one particle when present in the at least one microfluidic structure.

In example implementations, an apparatus is provided. The apparatus includes a channel, an energy source, and a transfection chamber. The channel includes an indentation to hold a cell. The energy source is to apply a shockwave to the cell in the channel to porate the cell. The transfection chamber is to store a reagent to be inserted into the cell after the cell is porated.