Fluidic devices, systems, and methods for analyzing an analyte are described. In an embodiment, the fluidic devices include a housing defining a lysis chamber shaped to receive a biological sample; a lysis buffer storage chamber disposed within the housing and carrying a lysis buffer configured to lyse cells of the biological sample; a cap configured to cooperatively couple to the housing; a compressor configured to compress the lysis buffer storage chamber and expel the lysis buffer from the lysis buffer storage chamber and into the lysis chamber when the cap is uncoupled from the housing; and a porous membrane in selective fluidic communication with the lysis chamber.

The invention provides a microfluidic system comprising a cartridge coupled to a motor and adapted to move a fluid sample to a plurality of locations on the cartridge.

A system for processing a sample includes a unitary body. The unitary body including a snap lid, the snap lid having a capillary. The unitary body including a lysing container. The unitary body including a test element and a sliding actuator.

A microfluidic arrangement for manipulating fluids is provided. The microfluidic arrangement comprises a substrate, a first fluid and a second fluid, which is immiscible with the first fluid. The first fluid is arranged to be at least partially covered by the second fluid. The first fluid is arranged in a desired shape on an unpatterned surface of the substrate. The first fluid is retained in said shape by a fluid interface between the first and second fluids. A microfluidic arrangement comprising an array of drops is also provided. The microfluidic arrangement comprises a substrate, a first fluid and a second fluid, which is immiscible with the first fluid. The first fluid is arranged to be at least partially covered by the second fluid. The first fluid is arranged to be covered at least partially by the second fluid. The first fluid is arranged in a given array of drops on an unpatterned surface of the substrate. Each drop cross section area having a (height:width) aspect ratio of (1:2) or less. A method of fabricating a microfluidic arrangement for manipulating fluids is also provided. The method comprises arranging a first fluid on an unpatterned surface of a substrate in a desired shape. The method also comprises arranging a second fluid, which is immiscible with the first fluid, to cover the first fluid at least partially. The first fluid is retained in said shape by a fluid interface between the first and second fluids. The method also comprises drying the first fluid to form a residue in said shape on the substrate.

A system and method for receiving and delivering a fluid, the system comprising: a body configured to interface with an opening of a reservoir and defining: a protrusion defining a set position of the body relative to the reservoir; a wall extending from the protrusion; a receiving surface coupled to the wall and sloping from an apex to a nadir along a first direction, the receiving surface comprising a vent; and an outlet positioned closer to the nadir than the apex of the receiving surface and displaced from the vent, the outlet comprising an extension from the body, the extension configured to contact an interior wall of the reservoir, wherein the body comprises: a bubble-mitigating operation mode in which the receiving surface receives and transmits the fluid along the receiving surface, and a fluid-transmitting operation mode in which the body directs the fluid along the interior wall of the reservoir.

A system and method for receiving and delivering a fluid, the system comprising: a body configured to interface with an opening of a reservoir and defining: a protrusion defining a set position of the body relative to the reservoir; a wall extending from the protrusion; a receiving surface coupled to the wall and sloping from an apex to a nadir along a first direction, the receiving surface comprising a vent; and an outlet positioned closer to the nadir than the apex of the receiving surface and displaced from the vent, the outlet comprising an extension from the body, the extension configured to contact an interior wall of the reservoir, wherein the body comprises: a bubble-mitigating operation mode in which the receiving surface receives and transmits the fluid along the receiving surface, and a fluid-transmitting operation mode in which the body directs the fluid along the interior wall of the reservoir.

The invention provides devices that improve tests for detecting specific cellular, viral, and molecular targets in clinical, industrial, or environmental samples. The invention permits efficient detection of individual microscopic targets at low magnification for highly sensitive testing. The invention does not require washing steps and thus allows sensitive and specific detection while simplifying manual operation and lowering costs and complexity in automated operation. In short, the invention provides devices that can deliver rapid, accurate, and quantitative, easy-to-use, and cost-effective tests.

Systems, devices, compositions and methods for positioning and/or processing a target are provided. The invention includes systems, devices, methods and related compositions useful, for example, for the separation, isolation, purification, identification, detection and quantification of materials. Also provided are systems and methods for isolation, and/or detection and/or quantification of a target or analyte in a sample. Some systems, devices, compositions and methods comprise oil and aqueous phases stabilized in close proximity to each other. Some systems, devices and methods use a magnetic force to draw a target or carrier-bound through multiple layers. In some embodiments, systems and devices comprise reagents for detection of a target or analyte.

The present disclosure provides novel gravitational flow assays for detecting an antigen in a biological sample using DNA-capture sequences. Specifically, gravitational flow assays and methods of detecting viruses, including coronaviruses, are provided.

One aspect of this invention relates to a vertical-via rotary valve including a valve body having a housing; one or more fluidic channels, each fluidic channel having a vertical channel portion defined in the valve body and being adjacent to the housing, wherein a fluid flow through the vertical channel portion is controllable by deforming a sidewall of the vertical channel portion; and an actuator received in the housing and rotatably engaged with the one or more fluidic channels to operably control the fluid flow through the vertical channel portion of each fluid channel.