Provided herein are microfluidic circuits that include at least one device for forming a quantity of drops of a solution in a carrier fluid and at least one storage zone for storing drops produced by the microfluidic device. Such microfluidic circuits are useful, for example, for the analysis of a solution containing a biological sample.

The disclosed apparatus, systems and methods relate to the collection of bodily fluids through the use of gravity and microfluidic properties by way of a collector. The collector can make use of microfluidic networks connected to collection sites on the skin of a subject to gather and shuttle blood into a reservoir by a combination of capillary action and gravitational forces. The collected fluid is moved through the microfluidic networks and into the reservoir by a variety of approaches.

Non-limiting embodiments of a modified devices that comprise at least one dye for determining whether results obtained from the conductance of at least one diagnostic assay are biased, as well as kits and methods of use related thereto.

In general the present invention concerns 1) single cell trapping of a viable cell in separate well from a plurality of wells in an array of wells, 2) single cell analysis for the selected cell and 3) single cell lifting of the yet viable cell from the well by an optical tweezer. Furthermore resent invention concerns a cell trap and lift device for B lymphocytes, the device comprising an array of wells in in polymer matrix comprising an off-stoichiometry thiol-ene polymer of the group consisting of off-stoichiometry thiol-ene (OSTE) and off-stoichiometry thiol-ene-epoxy (OSTE+) or a combination thereof that have been grafted with methacrylated polyethylene glycol (methoxy polyethylene glycol methacrylate or (M-PEG-M)) of a number average molecular weight of Mn 2000. It furthermore concerns using the B lymphocyte trap and lift device for trapping single B lymphocyte cells in wells of the device of present invention and lifting said cell from the cell trapping well by optical tweezers, preferably single beam tweezers.

Disclosed are a microfluidic chip (1000) and a manufacturing method therefor. The microfluidic chip comprises a substrate (100) and a detection area (2) located on the substrate (100), the substrate (100) is provided with a first liquid storage groove (11) and a second liquid storage groove (12), the first liquid storage groove (11) and the second liquid storage groove (12) are in liquid communication with the detection area (2), the first liquid storage groove (11) is provided with a first opening (51) for liquid to flow out, and the second liquid storage groove (12) is provided with a second opening (52) for liquid to flow out; and when the microfluidic chip (1000) is used for sample detection, along with rotation of the microfluidic chip (1000), a rear end of the liquid flowing out of the first liquid storage groove (11) reaches the detection area (2) earlier than a front end of the liquid flowing out of the second liquid storage groove (12).

Disclosed herein are solvent reservoir systems for steady flow delivery and simultaneous monitoring of solvent level and solvent density within the solvent reservoir systems and methods for monitoring the solvent reservoir systems and providing feedback to a user or adjusting the systems in response to the monitored characteristics.

A device is described that overlays a first fluid, such as blood or a cellular suspension onto a base material, such as a density gradient. In some embodiments, the fluid layering device includes a cylindrical reservoir, a fluid barrier, a coupling extension, a plunger, and an exhaust vent. The fluid layering device can be coupled through its coupling extension to an open end of a container, such as a conical centrifuge tube, including the density gradient. Once attached, the plunger may be lowered to a position above the surface of the density gradient. A first fluid may flow from the reservoir into the conical tube across the plunger, so that a suitable overlay is formed without substantially disturbing a surface of the density gradient.

The purpose of the present invention is to provide a liquid handling method that makes it possible to simply isolate a droplet, a fluid handling device used in this method, and a fluid handling system. This fluid handling method serves to flow a fluid including a solvent and a droplet through a fluid handling device comprising an inlet, a first flow path having one end connected to the inlet, a chamber connected to the other end of the first flow path, a second flow path having one end connected to the chamber, and an outlet connected to the other end of the second flow path. This fluid handling method includes an introduction step for introducing the fluid through the inlet and accommodating the droplet in the chamber, a rotation step for rotating the fluid handling device after the introduction step, and a removal step for removing the droplet from the inside of the chamber after the rotation step. In the introduction step, the fluid handling device is disposed such that the chamber widens from the position where the chamber and the second flow path are connected in the direction opposite from the direction of gravity.

Methods and systems are provided for point-of-care nucleic acid amplification and detection. One embodiment of the point-of-care molecular diagnostic system includes a cartridge and an instrument. The cartridge can accept a biological sample, such as a urine or blood sample. The cartridge, which can comprise one or more of a loading module, lysis module, purification module and amplification module, is inserted into the instrument which acts upon the cartridge to facilitate various sample processing steps that occur in order to perform a molecular diagnostic test.

An apparatus and method for collecting multi-tiered particles from various biological samples for use in downstream biological procedures. Two filters compatible with the device, and of different pore size, are connected via a conduit or are integrated into the conduit. A biological sample is driven through the conduit by applying pressure to one of the inlet ports. The flow of the biological sample is directed to the desired output port by positioning of the internal switch. A switch directs the fluid to flow within the device to elute trapped particles captured on the filters. The apparatus allows for the collection of unstimulated, intact particles without the need for multiple redraws of sample or filtrate. Collection and analysis of biological samples are valuable for correct diagnosis and therapy of a wide variety of different conditions.