The invention provides sample cartridges for processing samples. The sample cartridges comprise at least one fluidic channel. Each fluidic channel comprises a sample chamber, a lysis chamber, a binding chamber, a pre-amplification region, and an amplification region. The sample cartridges also comprise a waste line that is in fluidic connectivity with each fluidic channel. The sample cartridges can interface with a plurality of plungers that are capable of occluding at least one fluidic channel, waste line, and/or optional assay line to limit the transport of fluids into, out of, and/or along at least one fluidic channel by plunging. The invention also provides multi-channel sample cartridges, which are sample cartridges that comprise at least two fluidic channels. In addition, the sample cartridges can house fluids on the cartridge, off the cartridge, or some on the cartridge and some fluids off the cartridge.

The present invention provides self-contained systems for performing an assay for determining a chemical state, the system including a stationary cartridge for performing the assay therein, at least one reagent adapted to react with a sample; and at least one reporter functionality adapted to report a reaction of the at least one reagent with said sample to report a result of the assay, wherein the at least one reagent, the sample and the at least one reporter functionality are contained within the cartridge.

A point-of-care diagnostic system that includes a cartridge and a reader. The cartridge can contain a patient sample, such as a blood sample. The cartridge is inserted into the reader and the patient sample is analyzed. The reader contains various analysis systems, such as an electrophoresis detection system that uses electrophoresis testing to identify and quantify various components of the blood sample. The reader can process data from the various patient sample analysis to provide interpretative results indicative of a disorder, condition, disease and/or infection of the patient.

Function fabrication in a microfluidic device manufactured with a custom 3D printer. The functions may include, for example, transporting or routing fluid, fluid mixing through flow and/or diffusion, blocking fluid (valve), pumping fluid, providing chemical reaction regions, providing analyte capture regions, and providing analyte separation regions. The fluid may be a liquid or a gas.

A device for precise dispensing of liquids utilizing pressure-operated valves to control fluid travel. Use of a flexible bulb within a fluid vessel provides manual actuation of the valves through application of pressure to the exterior of the fluid vessel.

A flow cell having at least one storage zone connected to a duct for conducting fluid out of, into or/and through the storage zone. The duct includes a duct section which is delimited by a substrate and a film joined to the substrate and in which the duct is sealed and can be opened at a predetermined breaking point by deflecting the film. The film covers a recess in the substrate which forms the duct section. A sealing wall that seals the duct and is integrally joined to the substrate is placed in the recess. The predetermined breaking point is formed by a breakable joining region between the film and an edge portion of the sealing wall facing the film. The dimensions of a peripheral area of the sealing wall is formed in the edge portion and runs parallel to the film determine the surface area of the joining region.

Storage and dispensing systems are provided. In various embodiments, systems of the present disclosure provide safety feature to prevent or limit the risk of a child or minor opening the system. Embodiments of the present disclosure further comprise novel cap and closure arrangements including, for example, features that are operable to automatically load or fill a pipette of the system.

A device enables a user to detect biomarkers, and includes an element that defines a multiplicity of microfluidic channels that communicate between an inlet duct and an outlet duct, the inlet duct communicating with an inlet port into which a user can introduce a drop of body fluid; the outlet duct communicating with an outlet port. A resilient bladder is connected to the outlet port to provide suction. Each microfluidic channel defines a reaction chamber containing a biomarker-sensitive reagent which provides a color or a change of color in the presence of a biomarker, there being a multiplicity of different biomarker-sensitive reagents, one such biomarker-sensitive reagent being provided in each of the multiplicity of different microfluidic channels. At least part of the element is transparent so the color within the reaction chamber can be seen. The device includes a cover with magnifying lenses above the reaction chambers. The device may be used in conjunction with a smart phone.

The present invention provides devices and methods for generating a pulsatile fluid flow in a microchannel by means of external actuation of a thin flexible film. With the devices described herein, cycles of positive and negative actuation can be used to infuse or withdrawal fluid in a microchannel. Fluid can be recirculated over one or more microfluidic feature, such as a chemical or molecular receptor, biosensor, electrode, cell or biological material, chromatography feature, mixer, etc., in a way that would represent an advantage over the single-pass flow techniques common to most microfluidic devices. The devices and methods are particularly useful in vitro diagnostics (IVD), analytical chemistry, chromatography, and mixing applications in a variety of fields.

Techniques regarding one or more structures that can facilitate automated, multi-stage processing of one or more nanofluidic chips are provided. For example, one or more embodiments described herein can comprise a system, which can comprise a roller positioned adjacent to a microfluidic card comprising a plurality of fluid reservoirs in fluid communication with a plurality of nanofluidic chips. An arrangement of the plurality of nanofluidic chips on the microfluidic card can defines a processing sequence driven by a translocation of the roller across the microfluidic card.