A fluidic device (10) is described. The fluidic device (10) comprises the first part (110) and the second part (120). The first part (110) comprises a first inlet (111) and a first outlet (112), mutually spaced apart. The second part (120) comprises a first chamber (121) arranged to contain a predetermined first amount A1 of a first fluid F1 therein and a first wall portion (122) arranged to contain, at least in part, the first fluid F1 in the first chamber (121). The fluidic device (10) is arrangeable in a first configuration, wherein the first part (110) is fluidically isolated from the first chamber (121). The fluidic device (10) is arrangeable in a second configuration, wherein the first inlet (111) and the first outlet (112) are fluidically coupled via the first chamber (121), whereby increasing a first pressure P1 in the first chamber (121) via the first inlet (111) urges at least a part of the predetermined first amount A1 of the first fluid F1 through the first outlet (112).

The objective of the present invention is to provide a particle detection device and a particle detection method that can individually and continuously detect a wide range of particles. The objective is achieved by a particle detection device including: a particle separation channel through which particles are separated according to particle sizes in a perpendicular direction to the flow of fluid; and two or more particle recovery channels that are connected to and branched from the particle separation channel, in which each of the particle recovery channels includes a particle detection unit that includes an aperture and an electric detector.

Embodiments of the invention provide a microfluidic chip having microfluidic structures formed on a surface. The structures form an input channel, an output channel, auxiliary channels, and a hydraulic resistor structure connecting the input channel to the output channel via the auxiliary channels. The resistor structure includes N flow resistor portions (N≥2), which are connected to the auxiliary channels. The chip further includes at least N−1 actuatable valves, which are arranged in respective ones of the auxiliary channels. The actuation state of the valves can determine the effective hydraulic resistance of the resistor structure. The valves can be electrogates, each including a liquid-pinning trench arranged in a respective one of the auxiliary channels that define a flow path for a liquid introduced therein, so as to form an opening that extends across said flow path. Each electrogate can further include an electrode extending across the flow path.

The present invention provides compositions, systems, kits, and methods for analyzing the interaction of T-cells and neoantigen presenting cells (and other cells) via discrete entity (e.g., droplet) microfluids. In certain embodiments, a microfluidic device is used to merge a discrete entity containing a T-cell, and a discrete entity containing a neoantigen presenting cell, at a merger region via a trapping element in order to generate a combined discrete entity. In particular embodiments, at least one thousand of such combined discrete entities are formed in about one second. In some embodiments, whether the receptor on the T-cell sufficiently binds the neoantigen to activate the T-Cell is detected (e.g., via detection of cytokine or granzyme B release). In certain embodiments, provided herein are methods for identifying polyfunctional T-cells or NK-cells, as well as methods of screening for such cells that would be cytotoxic if injected into a subject.

The invention relates to a spraying device, a cleaning machine for cleaning medical, pharmaceutical and/or laboratory utensils, a retrofitting device for retrofitting a hydraulic spraying device and a method for cleaning medical, pharmaceutical and/or laboratory utensils. At least one washing arm (3, 3a) is rotatably arranged on an axle (4) and can be supplied with cleaning fluid from a cleaning fluid supply line (5) in the axle (4). The washing arm (3, 3a) is provided with outlet openings (6) from which jets of cleaning fluid can be directed onto the utensils (8). The spraying device (2) has a drive device (9) for actively driving the washing arm (3, 3a). The drive device (9) comprises a turbine (11) which is in operative connection with the washing arm (3, 3a) in such a way that a rotation of the turbine (11) leads to a rotation of the washing arm (3, 3a). The drive device (9) also has a drive fluid line (10, 10a) separate from the cleaning fluid supply line (5). The turbine (11) can be driven by a drive fluid flowing out of the separate drive fluid line (10, 10a).

The present disclosure is related to a method of producing a microfluidic sorting apparatus. The method includes providing an injection-molded substrate comprising a network of channels; bonding an insulating film to an upper surface of the substrate to cover the network of channels; and depositing a conductive film on the insulating film. The substrate can be separated from the conductive film.

A microfluidic device comprises upper and lower spaced apart substrates defining a fluid chamber therebetween; an aperture for introducing fluid into the fluid chamber; a plurality of independently addressable array elements, each array element defining a respective region of the fluid chamber; and control means for addressing the array elements. The control means are configured to: determine that a working fluid has been introduced into a first region of the fluid chamber; and provide an output to a user to indicate that the working fluid is present in the first region. Once the working fluid is in the first region, the fluid applicator used to dispense the fluid can be removed without any risk of accidentally withdrawing dispensed working fluid from the microfluidic device. In the case of manual loading of the working fluid the output may inform a user that it is safe to remove the applicator, or in the case of automatic or robotic loading the output signal may be provided to the system controlling the automatic or robotic loading of fluid so that the system can remove the fluid applicator.

A liquid amount control device includes a position detector, a pump, a driver and a controller. The pump is connected with a first tip and configured to suck up the liquid out of the test tube. The driver is connected with the pump and configured to drive the pump to move. The controller is configured to: (1) control the driver to drive a first suction end of the first tip to enter a detection region of the position detector to obtain an end height position of the first suction end; (2) control the driver to drive the first suction end of the first tip to enter the liquid by a first entering depth according to the end height position; and (3) control the pump to suck up the liquid in the test tube through the first tip.

A passive, hydrodynamic technique implemented using a microfluidic device to perform co-encapsulation of samples in droplets and sorting of said droplets is described herein. The hydrodynamic technique utilizes laminar flows and high shear liquid-liquid interfaces at a microfluidic junction to encapsulate samples in the droplets. A sorting mechanism is implemented to separate sample droplets from empty droplets. This technique can achieve a one-one-one encapsulation efficiency of about 80% and can significantly improve the droplet sequencing and related applications in single cell genomics and proteomics.

A flow stabilized chip includes a chip mainbody, a buffering chamber and two fluid delivery ports. The chip mainbody has a pipe-connection surface. The buffering chamber is disposed in the chip mainbody. The two fluid delivery ports are disposed on the pipe connection surface and connected to the buffering chamber. The chip mainbody includes, in order from the pipe-connection surface to a bottom of the chip mainbody, a first base plate, a first elastic membrane, a second base plate, a second elastic membrane and a third base plate. The first base plate includes a first opening. The second base plate includes a second opening. The third base plate includes a third opening. The first elastic membrane, the second base plate and the second elastic membrane are stacked in sequence to form the buffering chamber.