A point-of-care testing (POCT) system comprising an analyzer, a measurement cartridge, and a calibration cartridge for calibrating the measurement cartridge is described. The measurement cartridge comprises at least one electrochemical sensor for measuring the one or more properties of the blood sample, and the calibration cartridge comprises a similar at least one electrochemical sensor and at least one sealed blister containing calibration fluid for calibrating the measurement cartridge. Examples of properties of the blood sample may be pH, blood gases, electrolytes, and metabolites like glucose and creatinine. The measurement cartridge may also comprise an optical chamber for measuring for example, bilirubin and hemoglobin species, for which the analyzer comprises stored calibration algorithms. The optical chamber may be disposed in any location in the measurement cartridge, for receiving a portion of the blood sample.

A fluid handling device according to an embodiment of the present invention is a fluid handling device that includes a substrate and a film bonded to the substrate and that is configured to process a fluid, the fluid handling device including: a channel; a well connected to the channel; a rotary membrane valve disposed between the channel and the well; and a rotary membrane pump connected to the channel. For example, in the fluid handling device according to the embodiment of the present invention, a filter is disposed in the well or between the well and the rotary membrane valve for separating a blood cell component.

The present invention relates to a microfluidic device 100 for image multiplexing. The microfluidic device 100 comprises a base structure 110 comprising an optical window 140 and a fluid well insert 120 coupled to the base structure 110. The fluid well insert 120 is configured to retain a microscope slide 130 for mounting of a biological sample 150 within the microfluidic device 100. The fluid well insert 120 is also configured to provide a fluid to said biological sample 150. A fluid well insert lid 160 coupled to the fluid well insert 120 is also provided.

The disclosed method of manufacturing may include positioning a membrane on top of a channeled layer where the channeled layer includes a shim portion that is dimensioned to limit the amount of compression appliable to the membrane. The membrane may be positioned at a juncture in the channeled layer. The method may next include positioning a transmission housing layer membrane and the channeled layer. The method may also include fastening the channeled layer, the membrane, and the transmission housing layer together. The channeled layer, the membrane, and the transmission housing layer may be held together with at least one fastening member. Various other methods, systems, and computer-readable media are also disclosed.

Microfluidic devices in which electrokinetic mechanisms move droplets of a liquid or particles in a liquid are described. The devices include at least one electrode that is optically transparent and/or flexible.

The invention relates to a microfluidic device and to a method for use thereof for the separation, purification and concentration of components of fluid media. The invention relates in particular to a microfluidic device and a method for processing blood samples. A microfluidic device is provided, comprising: a structured component (1), which is formed as a flat body; a microfluidic channel system (2), which is configured in the structured component (1); at least one component (3) applied to a surface of the structured component (1); at least one porous functional element (5); and at least one fluidic interface (4.1, 4.2, 4.3), which is arranged at the structured component (1), for supplying media into the microfluidic channel system (2).

Provided herein are instruments and cartridges for processing samples. The cartridges include fluidic circuits in which fluid movement can be regulated by diaphragm valves. In certain cartridges, deformable material providing a diaphragm contacts an interface in the instrument that actuates the diaphragm directly, without intervening actuation layer. Certain cartridges have a plurality of fluidic circuits and fluid distribution channels or pneumatic distribution channels configured to deliver fluids or pneumatic pressure to any of the fluidic circuits, selectively. Certain cartridges have compartments containing on-board reagents. Compartments can be closed by a film attached to a body the cartridge through a heat seal.

An analysis device and analysis system for testing a biological sample using a receivable cartridge having at least one actuator for actuating a valve of the cartridge. A pressurized gas supply is provided for supplying a pressurized working medium, and a connection element pneumatically connects the pressurized gas supply to the cartridge for supplying the cartridge with the pressurized working medium, so that the at least one actuator is pneumatically operated by the pressurized gas supply.

Disclosed is a liquid-sealed cartridge in which a liquid is transferred by a centrifugal force generated when the liquid-sealed cartridge is rotated around a rotation shaft, including: a liquid storage portion configured to store the liquid therein; a seal having an outer peripheral portion connected to the liquid storage portion, the seal being configured to seal the liquid storage portion; a flow path connected to the liquid storage portion via the seal, through which the liquid in the liquid storage portion is transferred by the centrifugal force in a direction away from the rotation shaft, wherein, when the seal receives a pressing force, the seal is inclined in a pressing direction, with one portion of the outer peripheral portion thereof remaining connected with the liquid storage portion, and the other portion of the outer peripheral portion being separated from the liquid storage portion.

A storage vial (100, 200) may include a vial body (110, 210) having a first end (111, 211) and a second end (112, 212) and defining an internal volume (113, 213) configured to contain a biological material (B) therein, a first valve (120, 220) positioned at the first end of the vial body, a second valve (130, 230) positioned at the second end of the vial body, a first conduit connector (160, 260) positioned at the first end of the vial body, and a second conduit connector (170, 270) positioned at the second end of the vial body. The resulting construction may allow for closed system direct transfer of biological material from the storage vial to another vessel using aseptic techniques.