The present invention relates to the field of biomedical technology and discloses a cartridge and a testing device. The cartridge comprises a sample lysis compartment, a first sample mixing compartment and a first PCR compartment; a first valve is disposed between the sample lysis compartment and the first sample mixing compartment, the first valve controls the flowing or blocking of the sample between the sample lysis compartment and the first sample mixing compartment; a fourth valve is disposed between the first PCR compartment and the first sample mixing compartment, the fourth valve controls the flow or blocking of the sample between the first sample mixing compartment and the first PCR compartment; a first reagent is provided in the first sample mixing compartment. In the compartment, nucleic acids in the sample mix with the first reagent and is then sent to the first PCR compartment for amplification.

Provided herein is a valve manifold comprising (a) an elastomer sheet attached to a plurality of magnetic pistons, wherein the magnetic pistons project from a first side of the elastomer sheet; (b) a foot component comprising a first surface and a plurality of shafts that orthogonally pass through the first surface; and (c) a body component comprising a second surface, a groove that laterally passes along the second surface, and a plurality of reservoir channels that orthogonally pass through the second surface, wherein the elastomer sheet is compressed between the foot component and the body component.

The present invention relates to a liquid handling and processing tool for analyzing a biological sample. Furthermore, the present invention relates to a fluid processing and detection module for processing a liquid sample and for detecting analyte in the sample.

Microfluidic systems, pumps, valves and applications of the same are provided. The microfluidic system may be a pump or a valve having a fluidic chip and an actuator controlling the opening and closing of the fluidic channel in the fluidic chip. The actuator may be disposed to tilt from the fluidic chip, forming a tilted-rotor peristaltic pump. Alternatively, the actuator may be a rolling ball actuator, and different fluidic chips may be used in different applications. For example, the fluidic chip may be a spiral pump chip having spiral channels, a rotary peristaltic pump chip having multiple output channels, or a multi-port valve chip having one port interconnected with multiple different ports. An analytical valve chip may switchably interconnect bioreactor and rinse/calibration input channels to sensor and waste output channels. The actuator of a random-access valve can move from one valve position to another without opening or closing intermediate ones.

A fluid handling device, comprising: a substrate including a first channel, a second channel and a partition wall formed between the first channel and the second channel; a film including a diaphragm, the film being disposed on the substrate so that the diaphragm faces the partition wall; and a sliding member slidable on the film while contacting with the film, the sliding member including a protrusion formed on an underside thereof, and the sliding member being disposed on the film with the underside facing the film, wherein: the sliding member is capable of switching between a first state and a second state by sliding on the film, wherein the protrusion is positioned so as not to face the partition wall with the diaphragm therebetween in the first state, and the protrusion is positioned so as to face the partition wall with the diaphragm therebetween in the second state.

There is provided a nucleic acid extraction method using a cartridge comprising: (a) a driving part of a nucleic acid extraction device is connected to a control rod module disposed in an inner space of the upper body of a piston and a rotation control module coupled to the lower body of the piston; (b) driving the rotation control module and the control rod module, sequentially sucking sample and reagents from the plurality of chambers separated from each other into an interior space, and discharging the mixture of the interior space into the chamber of the cartridge; and (c) driving the rotation control module and the control rod module to suck the reagent inside the master mix bead chamber of the cartridge into the interior space of the piston upper body and then discharge the mixed reagent to a nucleic acid amplification module.

A rotating seal-type liquid testing apparatus includes a lower cup component, an upper cup body, a top cover and a testing element. The lower cup component includes a lower cup body, a high liquid baffle, a low liquid baffle and a water-absorbing sealing plug. The low liquid baffle and the high liquid baffle divide a bottom of an inner cavity of the lower cup body into a reaction region and a cut-off region. An edge of a bottom surface of the inner cavity of the lower cup body is provided with a vent hole, and the vent hole is positioned in the cut-off region. The upper cup body is disposed in the lower cup body, a bottom surface of an inner cavity of the upper cup body is provided with a liquid outlet, and the liquid outlet is connected to the reaction region in the lower cup body.

A method is provided that includes introducing a fluid sample (19) into a fluid container (2, 502, 702) of a filtration assembly (20, 500, 720) and passing the fluid sample (19) through a porous filter (5, 705) by distally advancing a plunger (3, 610, 703) within the fluid container (2, 502, 702), thereby capturing, on or within the porous filter (5, 705) at least a portion of any particulate present in the fluid sample (19). Thereafter, a cavity (28, 628, 728) is created within the fluid container (2, 502, 702) between a distal end of the plunger and a distal end (49, 549, 749) of the fluid container (2, 502, 702) by proximally partially withdrawing the plunger (3, 610, 703) within the fluid container (2, 502, 702), while one or more vacuum-prevention openings (11, 711) are open. An extraction liquid (30) is prepared by introducing one or more extraction reagents (29) into the cavity (28, 628, 728) and bathing the porous filter (5, 705). The extraction liquid (30) is tested for the presence of a biological target. Other embodiments are also described.

A cartridge of the present invention is to be used in combination with a channel chip including a channel through which a liquid flows. The cartridge includes a reservoir for storing a liquid, in which a through hole configured to be connected to the channel is opened into a bottom part of the reservoir; a liquid discarding part for discarding the liquid in the reservoir; and a connection part which is disposed above the through hole, and connects the reservoir and the liquid discarding part to each other.

A liquid handling device includes: a cartridge including a first reservoir part in which a first reagent that is preservable in a non-frozen state is stored; and a channel chip including a second reservoir part in which a second reagent that should be preserved in a frozen state is stored, and a channel connected to the second reservoir part. The cartridge is attachable and detachable to and from the channel chip. The first reservoir part is connected to the channel when the cartridge is mounted in the channel chip.