The present invention relates to fluidic devices for compartmentalizing samples. In particular, the devices and related systems and methods allow for compartmentalization by using one or more first chambers connect by a first channel (e.g., where the cross-sectional dimension of the first channel is less than the cross-sectional dimension of at least one first chamber).

A separation container includes a separation tube receiving a sample therein, a first sedimentation part connected to an end portion of the tube, a particle in the sample being deposited by a centrifugal or agitating force, and a separating part provided in the tube and including at least one separating layer which selectively opens and closes the tube.

The invention provides a version of fluorescent in situ hybridization (FISH) in which all the steps are performed at physiological temperatures, i.e., body temperature, to detect and identify pathogenic bacteria in clinical samples. Methods of the invention use species-specific fluorescent probes to label clinically important infectious bacteria. A sample such as a urine sample is loaded into a cartridge, fluorescently labeled, and imaged with a microscope. Labelled bacteria are pulled down onto an imaging surface and a dye cushion is used to keep unbound probes off of the imaging surface. A microscopic image of the surface shows whether and in what quantities the infectious bacteria are present in the clinical sample.

The present disclosure provides a microfluidic device configured to deliver a controlled amount of a liquid to an analysis tool, wherein the microfluidic device comprises: a buffer tank configured to contain a liquid and/or a gas; at leas one level sensor configured to measure a liquid level in the buffer tank; a pneumatic system configured to create selectively a positive or a negative pressure in the buffer tank; at least one intake port to let in a liquid in the buffer tank; at least one delivery port to inject a controlled amount of liquid from the buffer tank onto the analysis tool; at least one drain port with a controlled drain valve, located on the lower part of the buffer tank to discharge the liquid from the buffer tank; a first check valve located upstream of the intake port and a second check valve between the buffer tank and the analysis tool.

The present invention relates to fluidic devices for preparing, processing, storing, preserving, and/or analyzing samples. In particular, the devices and related systems and methods allow for preservation or storage of samples (e.g., biospecimen samples) by using one or more of a bridge, a membrane, and/or a desiccant.

Described herein are three-dimensional (3-D) paper fluidic devices. The entire 3-D device is fabricated on a support layer formed from a single sheet of material and assembled by folding the support layer. The folded structure may be enclosed in an impermeable cover or package. Chemically sensitive particles may be disposed in the support layer for use in detecting analytes.

A device and a method for isolating a target from a biological sample are provided. The target is bound to solid phase substrate to form target bound solid phase substrate. The device includes a lower plate with an upper surface having a plurality of regions. The biological sample is receivable on a first of the regions. An upper plate has a lower surface directed to the upper surface of the lower plate. A force is positioned adjacent the upper plate and attracts the target bound solid phase substrate toward the lower surface of the upper plate. At least one of the upper plate and the lower plate is movable from a first position wherein the target bound solid phase substrate in the biological sample are drawn to the lower surface of the upper plate and a second position wherein the target bound solid phase substrate are isolated from the biological sample.

A system that includes a cartridge housing and a hollow transfer module, according to an embodiment is described herein. The cartridge housing further includes at least one sample inlet, a plurality of storage chambers, a plurality of reaction chambers, and a fluidic network. The fluidic network is designed to connect the at least one sample inlet, a portion of the plurality of storage chambers and the portion of the plurality of reaction chambers to a first plurality of ports located on an inner surface of the cartridge housing. The hollow transfer module includes a second plurality of ports along an outer surface of the transfer module that lead to a central chamber within the transfer module. The transfer module is designed to move laterally within the cartridge housing. The lateral movement of the transfer module aligns at least a portion of the first plurality of ports with at least a portion of the second plurality of ports.

Proposed are an all-in-one kit for on-site molecular diagnosis and a molecular diagnosis method using the same. The kit includes a first component including a sample pre-processing part, a nucleic acid adsorption part, and a nucleic acid amplification part, and a second component including a detection part. The sample pre-processing part includes a transfer member for transferring a lysis buffer where a nucleic acid is dissolved and functions to pre-processes a sample. The nucleic acid adsorption part is positioned on the sample pre-processing part and is configured to be slidable onto the nucleic acid amplification part. The nucleic acid amplification part is connected to the sample pre-processing part and functions to elute the nucleic acid from the nucleic acid adsorption part and to amplify the eluted nucleic acid. The detection part functions to transfer the nucleic acid amplified by the nucleic acid amplification part and to detect the nucleic acid.

A cassette assembly (1) comprises a cover (100), two cassette halves (200A, 200B) and a slider (300) comprising multiple test chambers (302). The cassette halves (200A, 200B) comprise waste tanks (230) in fluid connection with reservoirs (240), prefilled in one of the cassette halves (200A, 200B) with test agents. The particular design of the cassette halves (200A, 200B) enable forming predefined 5 volumes of liquid to achieve predefined concentrations of the test agents in the reservoirs (240) in one of the cassette halves (200A, 200B) and liquid in the reservoirs (240) in the other of the cassette halves (200A, 200B). Gradients of the test agents can then be established over the multiple test chambers (302).