A sample-to-answer microfluidic system and method including a microfluidic instrument with a detection system for detecting target assay products, data display, vertically oriented receiving member for receiving a microfluidic cartridge and an actuator assembly including a vertically oriented actuator. The system also includes a microfluidic cartridge with reagent pouches including a flow through reagent pouch, reagent wells in fluidic connection with one another. Methods of using the sample-to-answer microfluidic system are also provided.

A fluidic component intended to be associated with a heating module and in which there is formed a fluidic circuit including a fluidic channel intended for the circulation of a fluid, the fluidic channel including at least one passage for the fluid and a widened portion forming a location produced at the periphery of the passage, the component including a fluidic valve mechanism including at least an actuating element capable of expanding, trapped by at least one body made of a meltable compound in the location.