Embodiments are directed to providing access without determining an identity of a requester. A fixture may receive a rule pertaining to access to a floor of a building. The fixture may receive a request to access the floor of the building. The fixture may grant access to the floor based on a determination that the rule indicates that access to the floor should be granted.

The disclosure provides methods, devices, and kits for conducting a quantitative analysis of a whole blood sample. Various modifications to the disclosed methods, devices, and kits are described.

This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.

A cartridge reader is configured to carry out a diagnostic test on a fluid sample contained within a fluidic cartridge. The cartridge comprises first, second and third collapsible blisters containing at least one reagent for use in the diagnostic test. The cartridge reader comprises an upper clamp, occupying a fixed position relative to the reader and a lower clamp, movable relative to the upper clamp, and wherein the upper clamp and the lower clamp are configured to receive and hold a fluidic cartridge therebetween. First, second and third blister actuators are mounted on the upper clamp, for aligning with first, second and third collapsible blisters of a fluidic cartridge inserted into the reader. The first, second and third blister actuators are movable relative to the upper clamp, between a first position in which the blister actuators are spaced apart from the collapsible blisters comprised on the fluidic cartridge received between the upper and lower clamps, and a second position in which the blister actuators depress the collapsible blisters, thereby collapsing the blisters and ejecting the reagents contained therein into a channel in the microfluidic cartridge.

This present invention relates generally to devices, systems, and methods for performing optical and electrochemical assays and, more particularly, to devices and systems having universal channel circuitry configured to perform optical and electrochemical assays, and methods of performing the optical and electrochemical assays using the universal channel circuitry. The universal channel circuitry is circuitry that has electronic switching capabilities such that any contact pin, and thus any sensor contact pad in a testing device, can be connected to one or more channels capable of taking on one or more measurement modes or configurations (e.g., an amperometric measurement mode or a current drive mode).

A sample extraction chip and a biological reaction device are disclosed according to the present disclosure. The sample extraction chip includes a chip body and a sample extraction module provided on the chip body, the sample extraction module includes a sample-loading lysis unit, a liquid release-control unit, an extraction unit, a liquid switch-control unit, a liquid collection unit and a sample collection unit, which are connected through flow channels in a sequence of extraction. The liquid release-control unit is configured to store and release liquid reagents, and the liquid switch-control unit is configured to switch between communication of the liquid collection unit and the extraction unit and communication of the sample collection unit and the extraction unit. The sample collection unit includes a front collection portion and a rear collection portion which are both in communication with the liquid switch-control unit.

A patterned thermoplastic elastomer (TPE) film for fabricating a liquid-filled blister, has a blister-sized cavity in fluid communication with a microfluidic channel via a gating region. The gating region is defined by a relief pattern that has at least one of the following: at least 5 separate compartments defined by respective recesses in the first side, each of the recesses bounded by walls that separate the compartments from each other, the recess, or the channel; at least 5 walls defined by the patterning of the first side, the walls separating a plurality of compartments from each other, the recess, or the channel, wherein the walls have a mean thickness that is less than a mean height, and each pair of walls has a mean separation greater than twice the mean thickness; an array of separate compartments bounded by walls defined by the patterning of the first side that collectively define a polygonal regular planar tiling with at least 50% of the surface area of the gating region being open spaces; and a focusing region in fluid communication with the cavity, and a seal region having at least one wall defined by patterning of the film, wherein the at least one wall separates the focusing region from the seal region, and a shape of the at least one wall tapers the focusing region towards the seal region.

The present disclosure relates to a fluid analysis device which comprises a sensing device for analyzing a fluid sample, the sensing device comprising a micro-fluidic component for propagating the fluid sample and a microchip configured for sensing the fluid sample in the micro-fluidic component; a sealed fluid compartment containing a further fluid, the compartment being fluid-tight connected to the sensing device and adapted for providing the further fluid to the micro-fluidic component when the sealed fluid compartment is opened; and an inlet for providing the fluid sample to the micro-fluidic component. Further, the present disclosure relates to a method for sensing a fluid sample using the fluid analysis device.

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

The present invention provides integrated sample preparation systems and stabilized enzyme mixtures. In particular, the present invention provides microfluidic cards configured for processing a sample and generating DNA libraries that are suitable for use in sequencing methods (e.g., next generation sequencing methods) or other suitable nucleic acid analysis methods. The present invention also provides stabilized enzyme mixtures containing an enzyme (e.g., an enzyme used in whole genome amplification), BSA, and a sugar. Such enzyme mixtures may be lyophilized and stored at room temperature without significant loss of enzyme activity for months.