A pipette tip according to certain embodiments includes a proximal end, a distal end, and a reagent chamber. The proximal end is dimensioned to fit on an end of a pipettor, and includes a proximal end opening. The distal end includes a distal end opening. The reagent chamber has a reagent composition disposed therein. The reagent chamber is defined at least in part by a proximal barrier and a distal barrier. The reagent composition is operable to pass through the distal end opening upon rupturing of the distal barrier.

Provided herein are compositions, methods, assays, and devices for diagnosing and treating a viral infection in a subject and/or detecting a viral nucleic acid in a sample. In one example, a sample is treated in a first chamber and via a closing or twisting operation, the sample is flowed into a second chamber, where any pathogenic nucleic acid is detected by oligonucleotides that are specific to the pathogen under test. Further, the oligonucleotides comprise are cleavage site for a restriction enzyme in the second chamber, which cleaves oligonucleotide-pathogenic nucleic acid hybrid resulting in the exposure of an enzyme that was being held by the oligonucleotide to its substrate, which generates a colorimetric and/or another visual readout (e.g., foam formation). Further, the methods, device, or kits provided herein can be used to detect SARS-CoV-2 in a saliva sample from a human subject as a rapid in home diagnostic test for COVID-19.

A lateral flow device for analysing a sample of liquid has a first lateral flow strip configured to respond to a first volume of sample and a second lateral flow strip configured to respond to a second volume of the sample, wherein the second volume is greater than the first volume. The lateral flow device comprises a first well configured to receive the first volume of the sample and a second well configured to receive the second volume of the sample. Each well has a flow restriction outlet located adjacent its lateral flow strip. The lateral flow device comprises a pre-actuation configuration and an actuated configuration. The first and second flow restriction outlets are configured such that, in use, in the pre-actuation configuration, a first surface tension of the first volume of the sample within the first well prevents the first volume of the sample passing through the first flow restriction outlet and a second surface tension of the second volume of the sample within the second well prevents the second volume of the sample passing through the second flow restriction outlet. In the actuated configuration, (a) the first flow restriction outlet is located sufficiently proximate the first lateral flow strip such that the first volume of the sample makes contact with the first lateral flow strip and is drawn along the first lateral flow strip thereby overcoming the first surface tension; and (b) the second flow restriction outlet is located sufficiently proximate the second lateral flow strip such that the second volume of the sample makes contact with the second lateral flow strip and is drawn along the second lateral flow strip thereby overcoming the second surface tension.

In a device for coupling a cartridge for a lab-on-a-chip analysis device, the cartridge has at least one pneumatic port and at least one reagent chamber. The device has a receiving region and a clamping unit. The receiving region is shaped to receive the cartridge. The clamping unit includes a pneumatic interface for pneumatically contacting the pneumatic port and a punch for insertion into the reagent chamber. The clamping unit is arranged adjacent to the receiving region and is designed to perform a first translatory motion toward the receiving region in order to bring the pneumatic interface into contact with the pneumatic port. Furthermore, the clamping unit is designed to perform a second translatory motion toward the receiving region following the first translatory motion in order to insert the punch into the reagent chamber.

A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).

Diagnostic devices for performing diagnostic tests are provided, as well as methods that utilize the diagnostic devices, methods for manufacturing the diagnostic devices, and test kits for performing the diagnostic tests. The diagnostic devices include a sample chamber with an opening for receiving a sample; a fluid chamber containing a fluid; and a test and readout chamber containing a lateral-flow assay (LFA) strip. The fluid chamber and/or the test and readout chamber is/are burstable and is/are configured to be in fluid connection with the sample chamber upon bursting. The fluid chamber may be flexible and configured to burst at a seal separating the sample chamber from the fluid chamber. The seal maybe configured to break when a bursting force is applied to the fluid chamber.

An anti-pollution test device and a method for rapid test of nucleic acid amplifications, and a use. The anti-pollution test device includes a test portion and a mixing reaction chamber, where the mixing reaction chamber includes: a reaction chamber body, a reaction chamber cover, a channel and a mixing reaction portion. The test portion includes a test inner core, a test kit, a transparent observation window, a flow guide device, and the flow guide device including: a flow guide tube and a flow guide component. One end of the flow guide component is connected to the flow guide tube, one end of the flow guide component is connected to the test inner core, to guide a mixed solution of a hybridization solution and a sample to the test inner core. The test inner core displays a final test result in an area corresponding to the transparent observation window.

A glycated hemoglobin measurement kit including a reagent container, a cleaning solution, and a container holder. The reagent container has a cylindrical shape opened at opposite sides and includes a barrier membrane that blocks an opening at one side and forms a reagent storage part for a reagent for glycated hemoglobin measurement. The cleaning solution container has a cylindrical shape opened at opposite sides and includes an auxiliary barrier membrane that blocks an opening at one side and forms a cleaning solution storage part for a cleaning solution that cleans a reaction solution in which the blood and the reagent are mixed and react with each other. The container holder includes an attachment/detachment part from which a measurement strip is detachable and a measurement hole through which one area of the measurement strip attached to the attachment/detachment part is exposed.

An extraction portion which is a piece of a micro sampling chip is safely cut, and the cut extraction portion can be accommodated in a predetermined container without impairing quantitativeness of a sample. A sample extraction tool comprises a through hole for cutting the extraction portion from the micro sampling chip by bending the micro sampling chip while inserting the extraction portion to the through hole, the sample extraction tool is configured to be attached to an upper portion of a container for accommodating the extraction portion in such a state that the through hole is disposed on an open upper surface of the container.

A specimen sample collection device is disclosed, the device including: a base having a neck extending therefrom, a film-puncture element disposed within the neck, and one or more fluid ports extending through the base within the neck; a cap engaged with the neck, the cap comprising a film attached about an inner cylinder of the cap along a periphery thereof; wherein the film defines a buffer-containing cavity within the cap, and wherein the buffer-containing cavity is configured to hold a preservation buffer for preserving a sample stored within a collection reservoir of the device.