Modular active surface devices for micro fluidic systems and methods of making same is disclosed. In one example, the modular active surface device includes an active surface layer mounted atop an active surface substrate, a mask mounted atop the active surface layer wherein the mask defines the area, height, and volume of the reaction chamber, and a substrate mounted atop the mask wherein the substrate provides the facing surface to the active surface layer. In other examples, both facing surfaces of the reaction chamber include active surface layers. Further, the modular active surface device can include other layers, such as, but not limited to, adhesive layers, stiffening layers for facilitating handling, and peel-off sealing layers. Further, a large-scale manufacturing method is provided of mass-producing the modular active surface devices. Further, a method is provided of using a plasma bonding process to bond the active surface layer to the active surface substrate.

An example system includes an array of retaining features in a microfluidic cavity, an array of thermally controlled releasing features, and a controller coupled to each releasing feature in the array of releasing feature. Each retaining feature in the array of retaining features is to position capsules at a predetermined location, the capsules having a thermally degradable shell enclosing a biological reagent therein. Each releasing feature in the array of releasing features corresponds to a retaining feature and is to selectively cause degradation of the shell of a capsule. Each releasing feature is to generate thermal energy to facilitate degradation of the shell. The controller is to selectively activate at least one releasing feature in the array of thermally controlled releasing features to release the biological reagent in the capsules positioned at the retaining feature corresponding to the activated releasing feature.

The present invention relates to a microfluidic device (1) for cultivating cells, in particular for generating brain organoids, comprising at least two fluid channels (2) positioned essentially opposite to each other and a main chamber (3) located between the fluid channels (2), wherein the main chamber (3) comprises at least one preferably sealable access opening, and each of the at least two fluid channels (2) is fluidly connected to the main chamber (3) at at least one point of contact (4), wherein a slotted structure (5) is provided at each point of contact (4) separating the main chamber (3) from the respective fluid channel (2), wherein the slotted structure (5) is permeable to a liquid.

A biomolecule analysis kit includes a reaction container configured to perform an enzymatic reaction, the reaction container including a base portion which has a container-shaped portion and a low-adsorption structural portion which is provided on at least the inner surface of the container-shaped portion, the low-adsorption structural portion having an adsorption rate lower than the base portion at which at least one of a sample which becomes a target of analysis in the enzymatic reaction and a reagent for the enzymatic reaction is adsorbed thereonto, wherein a signal resulting from the enzymatic reaction is configured to be detected when the enzymatic reaction is performed in the reaction container.

A chip for blood plasma separation includes: (i) a body part, in which a sealed space through which blood can flow is integrally formed and the channel part and a ridge are alternately and continuously formed; (ii) an inflow part, which is disposed at an upper region of the body part into which the blood inflows; (iii) an outlet for discharging blood cells located at one side surface of the body part; and (iv) an outlet for discharging blood plasma located at the other side surface of the body part, in which the ridge is formed discretely, a chip array for blood plasma separation including the chip for blood plasma separation, a device for blood plasma separation including the chip for blood plasma separation and/or the chip array for blood plasma separation, and a method for blood plasma separation using the device.

The present invention provides a solid phase extraction method using a micro device having a dam forming portion including a dam, the solid phase extraction method comprising the steps of: (i) injecting a solvent and a filler into the micro device, moving the solvent to the dam forming portion, the dam allowing the solvent to flow therethrough and preventing the filler from passing therethrough, and adsorbing a material to be separated onto the filler in the dam forming portion; and (ii) extracting, from the filler, the adsorbed material, wherein the micro device is rotated with respect to a central axis during one of steps (i) and (ii), and the rotation of the micro device is performed at an angular velocity defined by equation 1.

A liquid handling device has an accommodation part for accommodating a liquid, two or more flow paths each opening to a lower part of a side wall surface of the accommodation part, and a liquid movement suppression part that is disposed in the lower part of the side wall between the openings of two of the flow paths that are adjacent to each other and slows or stops the movement of the liquid along the corner formed by the lower surface of the accommodation part and the side wall surface.

Microfluidic systems and methods are described for capturing magnetic target entities bound to one or more magnetic beads. The systems include a well array device that includes a substrate with a surface that has a plurality of wells arranged in one or more arrays on the surface. A first array of wells is arranged adjacent to a first location on the surface. A second and subsequent arrays, if present, are arranged sequentially on the surface at second and subsequent locations. When a liquid sample is added onto the substrate and caused to flow, the liquid sample will flow across the first array first and then flow across the second and subsequent arrays in sequential order. The wells in the first array each have a size that permits entry of only one target entity into the well and each well in the first array has approximately the same size.

A particle manipulation system uses a spiral focusing channel to focus particles into a distribution near the centerline of the flow. The spiral focusing channel may have first portion and a second portion, wherein the first portion has a uniform cross section and curves in an arc of at least about 180 degrees, and the second portion has undulating sidewalls resulting in a varying cross section. The first portion may focus the particles substantially in a plane, and the second portion may focus the particles in a dimension orthogonal to the plane.

Techniques relate to forming a sorting device. A mesh is formed on top of a substrate. Metal assisted chemical etching is performed to remove substrate material of the substrate at locations of the mesh. Pillars are formed in the substrate by removal of the substrate material. The mesh is removed to leave the pillars in a nanopillar array. The pillars in the nanopillar array are designed with a spacing to sort particles of different sizes such that the particles at or above a predetermined dimension are sorted in a first direction and the particles below the predetermined dimension are sorted in a second direction.